Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Li, S.; Norioka, S.; Sakiyama, F.
    Bacteriolytic activity and specificity of Achromobacter beta-lytic protease (1998), J. Biochem., 124, 332-339.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.32 blp gene Achromobacter lyticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.32 1,10-phenanthroline 90% inhibition Achromobacter lyticus
3.4.24.32 2-mercaptoethanol 66% inhibition Achromobacter lyticus
3.4.24.32 additional information only 2% inhibition with EDTA, no inhibition with diisopropyl fluorophosphate, phenylmethylsulfonyl fluoride, iodoacetate, phosphoramidon, N-chloroacetyl-N-hydroxy-leucyl-Ala-Gly-NH2, chymostatin, elastinal, leupeptin, and pepstatin Achromobacter lyticus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.32 Zn2+ one zinc atom detected in the molecule by ion-spray mass spectrometry Achromobacter lyticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.32 19280
-
 acetonitrile-inactivated enzyme, ion spray mass spectrometry Achromobacter lyticus
3.4.24.32 19280
-
 calculated from the amino acid sequence deduced from the nucleotide sequence of the cloned blp gene Achromobacter lyticus
3.4.24.32 19350
-
 active enzyme, ion spray mass spectrometry Achromobacter lyticus

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.32 Achromobacter lyticus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.32 DYMGWMDF-NH2 + H2O
-
 Achromobacter lyticus DYMG + WMDF-NH2
-
 ?
3.4.24.32 GNLWATGHFM-NH2 + H2O
-
 Achromobacter lyticus GNLWATG + HFM-NH2
-
 ?
3.4.24.32 KFIGLM-NH2 + H2O
-
 Achromobacter lyticus KFIG + Leu-Met-NH2
-
 ?
3.4.24.32 L-pyroglutamyl-HWSYGLRPG-NH2 + H2O
-
 Achromobacter lyticus L-pyroglutamyl-HWSYG + LRPG-NH2
-
 ?
3.4.24.32 L-pyroglutamyl-LM-NH2 + H2O
-
 Achromobacter lyticus ?
-
 ?
3.4.24.32 L-pyroglutamyl-QRLGNQWAVGHLM-NH2 + H2O
-
 Achromobacter lyticus L-pyroglutamyl-QRLG + NQWAVGHLM-NH2
-
 ?
3.4.24.32 L-pyroglutamyl-VPQWAVGHFM-NH2 + H2O
-
 Achromobacter lyticus L-pyroglutamyl-VPQWAVG + HFM-NH2
-
 ?
3.4.24.32 RPPGFSPFR + H2O
-
 Achromobacter lyticus RPPG + FSPFR
-
 ?
3.4.24.32 WAGGDASGE + H2O
-
 Achromobacter lyticus WAGG + DASGE
-
 ?
3.4.24.32 WAGGNASGE + H2O
-
 Achromobacter lyticus WAGG + NASGE
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.32 Achromopeptidase component
-
 Achromobacter lyticus
3.4.24.32 beta-lytic metalloproteinase
-
 Achromobacter lyticus
3.4.24.32 beta-Lytic protease
-
 Achromobacter lyticus
3.4.24.32 blp
-
 Achromobacter lyticus
3.4.24.32 EC 3.4.24.4 formerly included in Achromobacter lyticus
3.4.24.32 EC 3.4.99.13 formerly Achromobacter lyticus
3.4.24.32 Myxobacter AL-1 proteinase I formerly EC 3.4.99.29 Achromobacter lyticus
3.4.24.32 Myxobacter beta-lytic proteinase
-
 Achromobacter lyticus
3.4.24.32 Myxobacter495 beta-lytic proteinase
-
 Achromobacter lyticus
3.4.24.32 Myxobacterium sorangium beta-lytic proteinase
-
 Achromobacter lyticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.32 9
-
 peptide substrates Achromobacter lyticus
3.4.24.32 10
-
 bacterial substrates Achromobacter lyticus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.24.32 7 10.5 exhibits higher bacteriolytic activity at pH 10.0 than at pH 7.0-9.0 Achromobacter lyticus