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Literature summary extracted from

  • Grimek, T.L.; Escalante-Semerena, J.C.
    The acnD genes of Shewenella oneidensis and Vibrio cholerae encode a new Fe/S-dependent 2-methylcitrate dehydratase enzyme that requires prpF function in vivo (2004), J. Bacteriol., 186, 454-462.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.79 propionate induces enzyme expression, enzyme is required for growth on propionate Vibrio cholerae serotype O1
4.2.1.79 propionate induces enzyme expression, enzyme is required for growth on propionate Shewanella oneidensis
4.2.1.79 PrpF seems to be an accessory protein required to prevent oxidative damage of the Fe/S center of active AcnD enzyme, or is involved in synthesis or repair of the Fe/S cluster present in AcnD Vibrio cholerae serotype O1
4.2.1.79 PrpF seems to be an accessory protein required to prevent oxidative damage of the Fe/S center of active AcnD enzyme, or is involved in synthesis or repair of the Fe/S cluster present in AcnD Shewanella oneidensis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.79 expression of His-tagged AcnD and PrpF in Escherichia coli BL21(DE3), functional complementation of an enzyme-deficient mutant of Salmonella enterica only by expression of both AcnD and PrpF Shewanella oneidensis
4.2.1.79 structural genetic organization of prp operon Vibrio cholerae serotype O1
4.2.1.117
-
 Shewanella oneidensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.117 EDTA complete loss of activity Shewanella oneidensis
4.2.1.117 ferricyanide complete loss of activity Shewanella oneidensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.79 Fe2+ AcnD enzyme possesses 1 unstable iron-sulfur center per monomer, absolutely required for activity, can be reconstituted by Fe2+ under reducing conditions Vibrio cholerae serotype O1
4.2.1.79 Fe2+ AcnD enzyme possesses 1 unstable iron-sulfur center per monomer, absolutely required for activity, can be reconstituted by Fe2+ under reducing conditions Shewanella oneidensis
4.2.1.117 Iron enzyme requires an intact Fe/S cluster for activity Shewanella oneidensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.117 94000
-
 x * 94000, SDS-PAGE and calculated Shewanella oneidensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.79 2-Hydroxybutane-1,2,3-tricarboxylate Vibrio cholerae serotype O1
-
 (Z)-But-2-ene-1,2,3-tricarboxylate + H2O
-
 ?
4.2.1.79 2-Hydroxybutane-1,2,3-tricarboxylate Shewanella oneidensis
-
 (Z)-But-2-ene-1,2,3-tricarboxylate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.79 Shewanella oneidensis
-
 gene acnD and prpF are both needed for full enzyme activity, they exist in place of or in addition to prpD
-
4.2.1.79 Vibrio cholerae serotype O1
-
 gene acnD and prpF are both needed for full enzyme activity, they exist in place of or in addition to prpD
-
4.2.1.117 Shewanella oneidensis
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
4.2.1.117 in presence of air, activity is lost over time Shewanella oneidensis

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.79 recombinant His-tagged AcnD and PrpF from Escherichia coli BL21(DE3) Shewanella oneidensis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.79 additional information
-
 substrate specificity Shewanella oneidensis
4.2.1.117 2.9
-
 pH 8.0, 37°C, substrate citrate Shewanella oneidensis
4.2.1.117 5
-
 pH 8.0, 37°C, substrate cis-aconitate Shewanella oneidensis
4.2.1.117 7.8
-
 pH 8.0, 37°C, substrate 2-methylcitrate Shewanella oneidensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.79 2-Hydroxybutane-1,2,3-tricarboxylate
-
 Vibrio cholerae serotype O1 (Z)-But-2-ene-1,2,3-tricarboxylate + H2O
-
 ?
4.2.1.79 2-Hydroxybutane-1,2,3-tricarboxylate
-
 Shewanella oneidensis (Z)-But-2-ene-1,2,3-tricarboxylate + H2O
-
 ?
4.2.1.79 2-Hydroxybutane-1,2,3-tricarboxylate i.e. 2-methylcitrate, best substrate Vibrio cholerae serotype O1 (Z)-But-2-ene-1,2,3-tricarboxylate + H2O i.e. 2-methyl-cis-aconitate ?
4.2.1.79 2-Hydroxybutane-1,2,3-tricarboxylate i.e. 2-methylcitrate, best substrate Shewanella oneidensis (Z)-But-2-ene-1,2,3-tricarboxylate + H2O i.e. 2-methyl-cis-aconitate ?
4.2.1.79 citrate
-
 Vibrio cholerae serotype O1 cis-aconitate + H2O
-
 ?
4.2.1.79 citrate
-
 Shewanella oneidensis cis-aconitate + H2O
-
 ?
4.2.1.79 additional information 2-methylisocitrate and isocitrate are no substrates for AcnD Vibrio cholerae serotype O1 ?
-
 ?
4.2.1.79 additional information 2-methylisocitrate and isocitrate are no substrates for AcnD Shewanella oneidensis ?
-
 ?
4.2.1.117 2-methylcitrate
-
 Shewanella oneidensis 2-methyl-cis-aconitate + H2O
-
 ?
4.2.1.117 cis-aconitate + H2O
-
 Shewanella oneidensis citrate
-
 ?
4.2.1.117 citrate
-
 Shewanella oneidensis cis-aconitate + H2O
-
 r
4.2.1.117 additional information no substrate: 2-methylisocitrate, isocitrate. Enzyme only catalyzes the first half of the aconitase-like reaction Shewanella oneidensis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.117 ? x * 94000, SDS-PAGE and calculated Shewanella oneidensis

Synonyms

EC Number Synonyms Comment Organism
4.2.1.79 PrpD
-
 Vibrio cholerae serotype O1
4.2.1.79 PrpD
-
 Shewanella oneidensis
4.2.1.117 AcnD
-
 Shewanella oneidensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.79 37
-
 assay at Shewanella oneidensis

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.117 4Fe-4S-center enzyme requires an intact Fe/S cluster for activity Shewanella oneidensis