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Literature summary extracted from
- New class of IMP cyclohydrolases in Methanococcus jannaschii (2002), J. Bacteriol., 184, 1471-1473.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.5.4.10 | expression in Escherichia coli | Methanocaldococcus jannaschii |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.5.4.10 | additional information | not inhibitory: EDTA at 2 mM, iodoacetamide at 20mM, XMP, at 0.05 mM, IMP, at 0.02 mM | Methanocaldococcus jannaschii |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.5.4.10 | 0.082 | - |
5-Formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide | - |
Methanocaldococcus jannaschii |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.4.10 | 21500 | - |
x * 21500, SDS-PAGE | Methanocaldococcus jannaschii |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.4.10 | Methanocaldococcus jannaschii | Q58043 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.4.10 | - |
Methanocaldococcus jannaschii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.4.10 | 5-formamido-1-(5-phosphoribosyl)imidazole-4-carboxamide | - |
Methanocaldococcus jannaschii | IMP + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.4.10 | ? | x * 21500, SDS-PAGE | Methanocaldococcus jannaschii |
| 3.5.4.10 | More | amino acid sequence analysis | Methanocaldococcus jannaschii |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.5.4.10 | 80 | - |
1 h, in air, no loss of activity | Methanocaldococcus jannaschii |
| 3.5.4.10 | 90 | - |
30 min, in air, 84% residual activity | Methanocaldococcus jannaschii |
| 3.5.4.10 | 100 | - |
30 min, in air, 30% residual activity | Methanocaldococcus jannaschii |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.5.4.10 | 6 | 8.5 | broad | Methanocaldococcus jannaschii |
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Release 2023.1 (January 2023)
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