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Literature summary extracted from

  • Krappmann, S.; Pries, R.; Gellissen, G.; Hiller, M.; Braus, G.H.
    HARO7 encodes chorismate mutase of the methylotrophic yeast Hansenula polymorpha and is derepressed upon methanol utilization (2000), J. Bacteriol., 182, 4188-4197.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
5.4.99.5 tryptophan heterotrophic positive effector Ogataea angusta
5.4.99.5 tryptophan heterotrophic positive effector Saccharomyces cerevisiae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.99.5 expression in a Saccharomyces cerevisiae aro7DELTA strain Ogataea angusta
5.4.99.5 overexpression in Hansenula polymorpha Ogataea angusta

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.4.99.5 tyrosine heterotrophic feedback inhibitor Ogataea angusta
5.4.99.5 tyrosine heterotrophic feedback inhibitor Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.99.5 additional information
-
 additional information sigmoid substrate saturation curve with S0.5: 16.7 mM for chorismate at 37°C and S0.5: 12 mM for chorismate at 37°C in presence of 0.1 mM tyrosine Ogataea angusta
5.4.99.5 1.6
-
 chorismate 37°C, in presence of 0.01 mM tryptophan Ogataea angusta

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.4.99.5 32000
-
 32000, SDS-PAGE Ogataea angusta
5.4.99.5 70000
-
 PAGE Ogataea angusta

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.99.5 Chorismate Ogataea angusta biosynthesis of aromatic amino acids Prephenate
-
 ?
5.4.99.5 Chorismate Saccharomyces cerevisiae biosynthesis of aromatic amino acids Prephenate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.4.99.5 Ogataea angusta Q9P4D8
-
-
5.4.99.5 Saccharomyces cerevisiae P32178
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.99.5
-
 Ogataea angusta

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.4.99.5 168
-
 Superdex 200pg pool Ogataea angusta

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.99.5 Chorismate
-
 Ogataea angusta Prephenate
-
 ?
5.4.99.5 Chorismate
-
 Saccharomyces cerevisiae Prephenate
-
 ?
5.4.99.5 Chorismate biosynthesis of aromatic amino acids Ogataea angusta Prephenate
-
 ?
5.4.99.5 Chorismate biosynthesis of aromatic amino acids Saccharomyces cerevisiae Prephenate
-
 ?

Subunits

EC Number Subunits Comment Organism
5.4.99.5 dimer 32000, SDS-PAGE Ogataea angusta

Synonyms

EC Number Synonyms Comment Organism
5.4.99.5 chorismate mutase
-
 Ogataea angusta
5.4.99.5 chorismate mutase
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.4.99.5 38
-
 decrease in turnover at temperatures higher than 38°C Saccharomyces cerevisiae
5.4.99.5 48
-
 maximum enzymatic activity Ogataea angusta

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.4.99.5 23.8
-
 chorismate 37°C, in presence of 0.1 mM tyrosine Ogataea angusta
5.4.99.5 89.3
-
 chorismate 37°C, in presence of 0.1 mM tyrosine Ogataea angusta
5.4.99.5 367
-
 chorismate 37°C Ogataea angusta