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Literature summary extracted from
- Studies of UDP-galactopyranose mutase from Escherichia coli: An unusual role of reduced FAD in its catalysis (2000), J. Am. Chem. Soc., 122, 9065-9070.
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.99.9 | 5-Deazariboflavin | specific activity 29fold higher than of control without illumination | Escherichia coli |  |
| 5.4.99.9 | Sodium dithionite | 20 mM | Escherichia coli | |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.4.99.9 | expression in Escherichia coli with a His-tag | Escherichia coli |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.9 | 0.022 | - |
UDP-D-galactofuranose | 37°C, pH 7.5, in presence of 20 mM sodium dithionite | Escherichia coli | |
| 5.4.99.9 | 0.194 | - |
UDP-D-galactofuranose | 37°C, pH 7.5 | Escherichia coli | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.9 | 44000 | - |
alpha2, 2 * 44000, calculated from translated peptide sequence | Escherichia coli |
| 5.4.99.9 | 72000 | - |
gel filtration | Escherichia coli |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.99.9 | Escherichia coli | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.4.99.9 | - |
Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.9 | UDP-D-galactopyranose | - |
Escherichia coli | UDP-D-galactofuranose | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.4.99.9 | homodimer | alpha2, 2 * 44000, calculated from translated peptide sequence | Escherichia coli |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.9 | 1.5 | - |
UDP-D-galactofuranose | 37°C, pH 7.5 | Escherichia coli | |
| 5.4.99.9 | 27 | - |
UDP-D-galactofuranose | 37°C, pH 7.5, in presence of 20 mM sodium dithionite | Escherichia coli | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.99.9 | FAD | 0.52 mol bound FAD per monomer | Escherichia coli | |
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Release 2023.1 (January 2023)
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