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Literature summary extracted from

  • Derbise, A.; Song, Y.P.; Parikh, S.; Fischetti, V.A.; Pancholi, V.
    Role of the C-terminal lysine residues of streptococcal surface enolase in Glu- and Lys-plasminogen-binding activities of group A streptococci (2004), Infect. Immun., 72, 94-105.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.11 expression in Escherichia coli strain XL1-Blue and BL-21 with N-terminal His-tag Streptococcus pyogenes

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.11 DELTA434-435 mutant with decreased Glu- and Lys-plasminogen-binding activities Streptococcus pyogenes
4.2.1.11 K434L/K435L mutant with decreased Glu- and Lys-plasminogen-binding activities Streptococcus pyogenes
4.2.1.11 K435L mutant with decreased Glu- and Lys-plasminogen-binding activities Streptococcus pyogenes
4.2.1.11 additional information replacement of the C-terminal lysine residues by leucine reduces Glu- and Lys-plasminogen-binding properties Streptococcus pyogenes

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.11 cell surface
-
 Streptococcus pyogenes 9986
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.11 2-phospho-D-glycerate Streptococcus pyogenes
-
 phosphoenolpyruvate + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.11 Streptococcus pyogenes
-
 type M6 strain D471
-
4.2.1.11 Streptococcus pyogenes
-
 M6 strain D471
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.11
-
 Streptococcus pyogenes

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.11 additional information
-
 his-tagged recombinant protein has the same activity than the wild type Streptococcus pyogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.11 2-phospho-D-glycerate
-
 Streptococcus pyogenes phosphoenolpyruvate
-
 r
4.2.1.11 2-phospho-D-glycerate
-
 Streptococcus pyogenes phosphoenolpyruvate + H2O
-
 r

Synonyms

EC Number Synonyms Comment Organism
4.2.1.11 SEN
-
 Streptococcus pyogenes
4.2.1.11 streptococcal surface enolase
-
 Streptococcus pyogenes
4.2.1.11 streptococcal surface enolase SEN Streptococcus pyogenes