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Literature summary extracted from

  • Ishihara, K.; Miura, T.; Kuramitsu, H.K.; Okuda, K.
    Characterization of the Treponema denticola prtP gene encoding a prolyl-phenylalanine-specific protease (dentilisin) (1996), Infect. Immun., 64, 5178-5186.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.B21 72 KDa and 43 KDa protein Treponema denticola

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.B21 4-mercuribenzenesulfonic acid 98% inhibition at 0.01 mM Treponema denticola
3.4.21.B21 diisopropyl fluorophosphates 97% inhibition at 1 mM Treponema denticola
3.4.21.B21 EDTA 41% inhibition at 5 mM Treponema denticola
3.4.21.B21 Soybean trypsin inhibitor 49% inhibition at 0.01 mM Treponema denticola
3.4.21.B21 ZnCl2 46% inhibition at 1 mM Treponema denticola

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.B21 38000
-
 three bands at 72000 Da, 43000 Da and 38000 Da after boiling, SDS-PAGE Treponema denticola
3.4.21.B21 43000
-
 three bands at 72000 Da, 43000 Da and 38000 Da after boiling, SDS-PAGE Treponema denticola
3.4.21.B21 72000
-
 three bands at 72000 Da, 43000 Da and 38000 Da after boiling, SDS-PAGE Treponema denticola
3.4.21.B21 98000
-
 two bands at 100000 Da and 98000 Da before boiling, SDS-PAGE Treponema denticola
3.4.21.B21 100000
-
 two bands at 100000 Da and 98000 Da before boiling, SDS-PAGE Treponema denticola

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.B21 Treponema denticola
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.B21 61.7
-
-
 Treponema denticola

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.B21 alpha1-antitrypsin + H2O
-
 Treponema denticola ?
-
 ?
3.4.21.B21 Fibronectin + H2O
-
 Treponema denticola ?
-
 ?
3.4.21.B21 Gelatin + H2O
-
 Treponema denticola ?
-
 ?
3.4.21.B21 immunogobulin G heavy chain + H2O weak substrate Treponema denticola ?
-
 ?
3.4.21.B21 additional information specificity for peptide bonds containing phenylalanine and proline at the P1 and P2 positions Treponema denticola ?
-
 ?
3.4.21.B21 N-succinyl-AAPL-4-nitroanilide + H2O synthetic substrate Treponema denticola ?
-
 ?
3.4.21.B21 N-succinyl-AAPN-4-nitroanilide + H2O synthetic substrate Treponema denticola ?
-
 ?
3.4.21.B21 Type IV collagen + H2O weak substrate Treponema denticola ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.B21 polymer x * 72000 + x * 38000 + x * 43000, 72000 Da active protease associates with smaller proteins, SDS-PAGE Treponema denticola

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.B21 8
-
-
 Treponema denticola

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.21.B21 Treponema denticola 72000 Da and 38000 Da protein, calculated from nucleic acid sequence
-
 5.42