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Literature summary extracted from

  • Rua, J.; Soler, J.; Busto, F.; de Arriaga, D.
    Specific and reversible inactivation of Phycomyces blakesleeanus isocitrate lyase by ascorbate-iron: role of two redox-active cysteines (2002), Fungal Genet. Biol., 35, 223-234.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.1 ascorbate enzyme is inhibited by an ascorbate plus Fe2+ system under aerobic conditions,16% residual activity at 2 mM ascorbate/0.02 mM Fe2+. Inactivation requires hydrogen peroxide, and can be prevented by catalase, EDTA, Mg2+, isocitrate, 20 mM glutathione, 20 mM dithiothreitol or 40 mM L-cysteine Phycomyces blakesleeanus
4.1.3.1 Fe2+ enzyme is inhibited by an ascorbate plus Fe2+ system under aerobic conditions,16% residual activity at 2 mM ascorbate/0.02 mM Fe2+. Inactivation requires hydrogen peroxide, and can be prevented by catalase, EDTA, Mg2+, isocitrate, 20 mM glutathione, 20 mM dithiothreitol or 40 mM L-cysteine Phycomyces blakesleeanus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.3.1 isocitrate Phycomyces blakesleeanus
-
 succinate + glyoxylate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.1 Phycomyces blakesleeanus
-
-
-

Storage Stability

EC Number Storage Stability Organism
4.1.3.1 removal of dithiothreitol during storage leads to spontaneous and slow inactivation Phycomyces blakesleeanus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.1 isocitrate
-
 Phycomyces blakesleeanus succinate + glyoxylate
-
 ?