EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.1.66 | overexpression of the wild-type and mutant enzymes in Escherichia coli XL1-Blue | Fusarium lateritium |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.2.1.66 | D275E | site-directed mutagenesis, 0-29% reduced activity compared to the wild-type enzyme | Fusarium lateritium |
4.2.1.66 | F170L | site-directed mutagenesis, inactive mutant compared to the wild-type enzyme | Fusarium lateritium |
4.2.1.66 | K136R | site-directed mutagenesis, 52% reduced activity compared to the wild-type enzyme | Fusarium lateritium |
4.2.1.66 | M302S | site-directed mutagenesis, 22% reduced activity compared to the wild-type enzyme | Fusarium lateritium |
4.2.1.66 | S13A | site-directed mutagenesis, 19-47% reduced activity compared to the wild-type enzyme | Fusarium lateritium |
4.2.1.66 | T12P | site-directed mutagenesis, inactive mutant | Fusarium lateritium |
4.2.1.66 | T12Q | site-directed mutagenesis, 10-48% reduced activity compared to the wild-type enzyme | Fusarium lateritium |
4.2.1.66 | V281A | site-directed mutagenesis, 8% reduced activity compared to the wild-type enzyme | Fusarium lateritium |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.66 | Fusarium lateritium | P32963 | - |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
4.2.1.66 | formamide = cyanide + H2O | Phe170 is an active site residue | Fusarium lateritium |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.2.1.66 | additional information | - |
cyanide hydratase and nitrilase activity | Fusarium lateritium |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.66 | acetonitrile + H2O | - |
Fusarium lateritium | ? | - |
? | |
4.2.1.66 | benzonitrile + H2O | best substrate for nitrilase activity | Fusarium lateritium | ? | - |
? | |
4.2.1.66 | HCN + H2O | - |
Fusarium lateritium | formamide | - |
? | |
4.2.1.66 | additional information | the enzyme also shows low nitrilase activity, which is sufficient for the fungus to grow on acetonitrile, propionitrile, or benzonitrile as sole nitrogen source | Fusarium lateritium | ? | - |
? | |
4.2.1.66 | propionitrile + H2O | - |
Fusarium lateritium | ? | - |
? |