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Literature summary extracted from

  • Ueda, S.; Sano, K.; Gao, D.; Tomihari, N.; Yamane, T.; Endo, I.
    Purification and properties of D-(-)-3-hydroxybutyrate oligomer hydrolase of Paracoccus denitrificans (2002), FEMS Microbiol. Lett., 206, 179-184.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.22 diisopropylfluorophosphate 98% inhibition at 1 mM Paracoccus denitrificans
3.1.1.22 iodoacetamide 11% inhibition at 1 mM Paracoccus denitrificans
3.1.1.22 NEM 13% inhibition at 1 mM Paracoccus denitrificans
3.1.1.22 PMSF 92% inhibition at 1 mM Paracoccus denitrificans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.22 0.7
-
 (R)-3-((R)-3-hydroxybutanoyloxy)butanoate pH 8.0, 30°C Paracoccus denitrificans
3.1.1.22 1
-
 (R)-3-((R)-3-hydroxybutanoyloxy)2butanoate pH 8.0, 30°C Paracoccus denitrificans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.22 30000
-
 gel filtration Paracoccus denitrificans
3.1.1.22 31000
-
 1 * 31000, SDS-PAGE Paracoccus denitrificans

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.22 Paracoccus denitrificans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.22 2350fold, to homogeneity Paracoccus denitrificans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.22 culture condition:ethanol-grown cell up to the mid-exponential growth phase, used for purification purpose Paracoccus denitrificans
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.22 additional information
-
 substrate specificity Paracoccus denitrificans
3.1.1.22 14.1
-
 purified enzyme Paracoccus denitrificans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.22 (R)-3-((R)-3-hydroxybutanoyloxy)2butanoate + H2O
-
 Paracoccus denitrificans (R)-3-hydroxybutanoate
-
 ?
3.1.1.22 (R)-3-((R)-3-hydroxybutanoyloxy)butanoate + H2O preferred substrate Paracoccus denitrificans (R)-3-hydroxybutanoate
-
 ?
3.1.1.22 4-nitrophenyl acetate + H2O 75% of the activity with 4-nitrophenyl butyrate Paracoccus denitrificans 4-nitrophenol + acetate
-
 ?
3.1.1.22 4-nitrophenyl butyrate + H2O best 4-nitrophenyl ester substrate Paracoccus denitrificans 4-nitrophenol + butyrate
-
 ?
3.1.1.22 4-nitrophenyl hexanoate + H2O 43% of the activity with 4-nitrophenyl butyrate Paracoccus denitrificans 4-nitrophenol + hexanoate
-
 ?
3.1.1.22 4-nitrophenyl pentanoate + H2O 60% of the activity with 4-nitrophenyl butyrate Paracoccus denitrificans 4-nitrophenol + pentanoate
-
 ?
3.1.1.22 4-nitrophenyl propionate + H2O 81% of the activity with 4-nitrophenyl butyrate Paracoccus denitrificans 4-nitrophenol + propionate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.22 monomer 1 * 31000, SDS-PAGE Paracoccus denitrificans

Synonyms

EC Number Synonyms Comment Organism
3.1.1.22 3HB oligomer hydrolase
-
 Paracoccus denitrificans
3.1.1.22 D-(-)-3-hydroxybutyrate oligomer hydrolase
-
 Paracoccus denitrificans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.22 40
-
-
 Paracoccus denitrificans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.22 30 45 15 min, 80% activity Paracoccus denitrificans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.22 8
-
-
 Paracoccus denitrificans

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.22 Paracoccus denitrificans isoelectric focusing
-
 5.2