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Literature summary extracted from

  • Gouda, M.; Yokogawa, T.; Asahara, H.; Nishikawa, K.
    Leucyl-tRNA synthetase from the extreme thermophile Aquifex aeolicus has a heterodimeric quaternary structure (2002), FEBS Lett., 518, 139-143.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.4 2 genes leuS and leuS', overexpression in Escherichia coli BL21(DE3) Aquifex aeolicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.4 0.0013
-
 tRNALeu from Escherichia coli recombinant enzyme complex, 55°C Aquifex aeolicus
6.1.1.4 0.0014
-
 tRNALeu from Aquifex aeolicus recombinant enzyme complex, 55°C Aquifex aeolicus
6.1.1.4 0.0083
-
 L-leucine recombinant enzyme complex, 65°C Aquifex aeolicus
6.1.1.4 0.228
-
 ATP recombinant enzyme complex, 65°C Aquifex aeolicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.4 35000
-
 heterodimer in quartenary structure, 2 * 35000, leuS gene product, + 2 * 65000, leuS gene product, SDS-PAGE Aquifex aeolicus
6.1.1.4 65000
-
 heterodimer in quartenary structure, 2 * 35000, leuS gene product, + 2 * 65000, leuS gene product, SDS-PAGE Aquifex aeolicus
6.1.1.4 100000
-
 complexed recombinant leuS' and leuS gene product, gel filtration Aquifex aeolicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.4 ATP + L-leucine + tRNALeu Aquifex aeolicus
-
 AMP + diphosphate + L-leucyl-tRNALeu
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.4 Aquifex aeolicus
-
 class I enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.4 recombinant enzymes from Escherichia coli, leuS' gene product to homogeneity Aquifex aeolicus

Renatured (Commentary)

EC Number Renatured (Comment) Organism
6.1.1.4 renaturation and refolding of recombinant leuS gene product expressed in Escherichia coli Aquifex aeolicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.4 ATP + L-leucine + tRNALeu
-
 Aquifex aeolicus AMP + diphosphate + L-leucyl-tRNALeu
-
 ?
6.1.1.4 ATP + L-leucine + tRNALeu enzyme activity only appears when both gene products, of leuS and leuS', coexist Aquifex aeolicus AMP + diphosphate + L-leucyl-tRNALeu
-
 ?
6.1.1.4 ATP + L-leucine + tRNALeu from Aquifex aeolicus
-
 Aquifex aeolicus AMP + diphosphate + L-leucyl-tRNALeu
-
 ?
6.1.1.4 ATP + L-leucine + tRNALeu from Escherichia coli
-
 Aquifex aeolicus AMP + diphosphate + L-leucyl-tRNALeu
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.4 tetramer heterodimer in quartenary structure, 2 * 35000, leuS' gene product, + 2 * 65000, leuS gene product, SDS-PAGE Aquifex aeolicus

Synonyms

EC Number Synonyms Comment Organism
6.1.1.4 Leucine translase
-
 Aquifex aeolicus
6.1.1.4 Leucine--tRNA ligase
-
 Aquifex aeolicus
6.1.1.4 Leucyl-transfer ribonucleate synthetase
-
 Aquifex aeolicus
6.1.1.4 Leucyl-transfer ribonucleic acid synthetase
-
 Aquifex aeolicus
6.1.1.4 Leucyl-transfer RNA synthetase
-
 Aquifex aeolicus
6.1.1.4 Leucyl-tRNA synthetase
-
 Aquifex aeolicus
6.1.1.4 LeuRS
-
 Aquifex aeolicus
6.1.1.4 Synthetase, leucyl-transfer ribonucleate
-
 Aquifex aeolicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.4 0.003
-
 tRNALeu from Escherichia coli recombinant enzyme complex, 55°C Aquifex aeolicus
6.1.1.4 0.006
-
 tRNALeu from Aquifex aeolicus recombinant enzyme complex, 55°C Aquifex aeolicus
6.1.1.4 0.094
-
 ATP recombinant enzyme complex, 65°C Aquifex aeolicus
6.1.1.4 0.134
-
 L-leucine recombinant enzyme complex, 65°C Aquifex aeolicus

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.4 ATP
-
 Aquifex aeolicus