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Literature summary extracted from

  • Mora, P.; Rubio, V.; Cervera, J.
    Mechanism of oligomerization of Escherichia coli carbamoyl phosphate synthetase and modulation by the allosteric effectors. A site-directed mutagenesis study (2002), FEBS Lett., 511, 6-10.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.5.5 ornithine
-
 Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.5.5 expression in Escherichia coli Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
6.3.5.5 H975L mutation abolishes oligomer formation even high enzyme concentrations or in the presence of ornithine Escherichia coli
6.3.5.5 H975L/N987V mutation abolishes oligomer formation even high enzyme concentrations or in the presence of ornithine Escherichia coli
6.3.5.5 L421E mutation prevents tetramer but not dimer formation Escherichia coli
6.3.5.5 L421E/H975L/N987V mutation abolishes oligomer formation even high enzyme concentrations or in the presence of ornithine Escherichia coli
6.3.5.5 L990A mutation abolishes oligomer formation even high enzyme concentrations or in the presence of ornithine Escherichia coli
6.3.5.5 N987V mutation decreases the oligomerisation Escherichia coli
6.3.5.5 N992A mutation abolishes oligomer formation even high enzyme concentrations or in the presence of ornithine Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.5.5 UMP
-
 Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.5 Escherichia coli
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.5.5 additional information
-
 constant specific activity of wild-type enzyme irrespective of the enzyme concentration or of the elution position of the enzyme in the gel filtration system under conditions at which different oligomeric forms predominate Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.5 2 ATP + L-Gln + HCO3-
-
 Escherichia coli 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.5.5 dimer at low concentrations in presence of UMP Escherichia coli
6.3.5.5 More in absence of effectors the enzyme behaves as a dissociating system possibly reflecting the dimer-tetramer equilibrium Escherichia coli
6.3.5.5 tetramer at high concentrations in presence of ornithine Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.3.5.5 0.004
-
 UMP pH 8.0, 24°C Escherichia coli