EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.4.11 | AtPLC2, full-length and deletion variants, expression in Escherichia coli BL21(DE3)pLys | Arabidopsis thaliana |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.1.4.11 | additional information | analysis of N-terminal deletion mutants of AtPLC2 | Arabidopsis thaliana |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.1.4.11 | plasma membrane | predominantly localized in | Arabidopsis thaliana | 5886 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.4.11 | Ca2+ | AtPLC2 requires low micromolar levels of Ca2+, maximal phosphatidylinositol 4,5-bisphosphate hydrolysis at 0.001 mM free Ca2+, hydrolysis of phosphatidylinositol requires higher Ca2+ concentrations | Arabidopsis thaliana |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.4.11 | 66142 | - |
x * 95000, recombinant GST-AtPLC2, SDS-PAGE, x * 66142, AtPLC2, calculated from the amino acid sequence | Arabidopsis thaliana |
3.1.4.11 | 95000 | - |
x * 95000, recombinant GST-AtPLC2, SDS-PAGE, x * 66142, AtPLC2, calculated from the amino acid sequence | Arabidopsis thaliana |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.4.11 | additional information | Arabidopsis thaliana | AtPLC2 is constitutively expressed | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.4.11 | Arabidopsis thaliana | Q39033 | 4 weeks old, AtPLC2 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.4.11 | recombinant AtPLC2 | Arabidopsis thaliana |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.4.11 | flower | AtPLC2 expression | Arabidopsis thaliana | - |
3.1.4.11 | leaf | AtPLC2 expression | Arabidopsis thaliana | - |
3.1.4.11 | shoot | AtPLC2 expression | Arabidopsis thaliana | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.4.11 | additional information | - |
60-350times higher specific activity with phosphatidylinositol 4,5-bisphosphate than with phosphatidylinositol as substrate | Arabidopsis thaliana |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.4.11 | 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O | AtPLC2, preferred substrate, N-terminal EF-hand-like domain is required for catalytic activity but not for lipid binding | Arabidopsis thaliana | 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol | - |
? | |
3.1.4.11 | additional information | AtPLC2 is constitutively expressed | Arabidopsis thaliana | ? | - |
? | |
3.1.4.11 | phosphatidylinositol + H2O | 60-350times lower specific activity than with phosphatidylinositol 4,5-bisphosphate | Arabidopsis thaliana | myo-inositol 1-phosphate + diacylglycerol | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.4.11 | ? | x * 95000, recombinant GST-AtPLC2, SDS-PAGE, x * 66142, AtPLC2, calculated from the amino acid sequence | Arabidopsis thaliana |