Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Nosal, F.; Masson, A.; Legrand, R.; Blanot, D.; Schoot, B.; van Heijenoort, J.; Parquet, C.
    Site-directed mutagenesis and chemical modification of the two cysteine residues of the UDP-N-acetylmuramoyl:L-alanine ligase of Escherichia coli (1998), FEBS Lett., 426, 309-313.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.8 expressed in Escherichia coli JM83 and PC2453 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
6.3.2.8 C230A activity completely lost at 42°C Escherichia coli
6.3.2.8 C426A Km increased 3fold for ATP and 10fold for UDP-MurNAc and L-alanine, with respect to the wild-type Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.8 0.01
-
 UDP-N-acetylmuramate wild-type enzyme Escherichia coli
6.3.2.8 0.04
-
 L-alanine wild-type enzyme Escherichia coli
6.3.2.8 0.3
-
 L-alanine C230A mutant Escherichia coli
6.3.2.8 0.45
-
 ATP wild-type enzyme Escherichia coli
6.3.2.8 1.1
-
 UDP-N-acetylmuramate C230A mutant Escherichia coli
6.3.2.8 1.4
-
 ATP C230A mutant Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.2.8 53640
-
 mass spectrometry Escherichia coli
6.3.2.8 53750
-
 trifluoroacetic adduct by mass spectrometry Escherichia coli
6.3.2.8 53880
-
 trifluoroacetic adduct by mass spectrometry Escherichia coli
6.3.2.8 53990
-
 trifluoroacetic adduct by mass spectrometry Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.8 Escherichia coli
-
-
-

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.3.2.8 37
-
 28% loss of activity with previous remove of dithiothreitol Escherichia coli