Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Lai, X.; Shao, H.; Hao, F.; Huang, L.
    Biochemical characterization of an ATP-dependent DNA ligase from the hyperthermophilic crenarchaeon Sulfolobus shibatae (2002), Extremophiles, 6, 469-477.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.5.1.1 expression in Escherichia coli Saccharolobus shibatae

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.5.1.1 KCl 100 mM, 85% inhibition, not inhibited below 50 mM Saccharolobus shibatae
6.5.1.1 NaCl 100 mM, 85% inhibition, not inhibited below 50 mM Saccharolobus shibatae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.5.1.1 0.034
-
 ATP 65°C, pH 6.5, recombinant DNA-ligase, in the presence of 1mM ATP and 5 mM Mn2+ Saccharolobus shibatae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.5.1.1 Ca2+ divalent cation required for activity, most effectiv cation together with Mg2+ for native DNA ligase, maximal activity at 5 mM Saccharolobus shibatae
6.5.1.1 Mg2+ divalent cation required for activity, most effectiv cation together with Ca2+ for native DNA ligase, maximal activity at 5 mM Saccharolobus shibatae
6.5.1.1 Mn2+ divalent cation required for activity, most effectiv cation for recombinant DNA ligase, 10times higher efficiency than with Mg2+, maximal activity at 2-5 mM Saccharolobus shibatae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.5.1.1 62000
-
 gel filtration Saccharolobus shibatae
6.5.1.1 67647
-
 1 * 67647, deduced from nucleotide sequence Saccharolobus shibatae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m Saccharolobus shibatae
-
 AMP + diphosphate + (deoxyribonucleotide)m+n
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.1 Saccharolobus shibatae Q9P9K9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.5.1.1 purification of recombinant DNA ligase, partially purification of native DNA ligase Saccharolobus shibatae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.1 ATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m
-
 Saccharolobus shibatae AMP + diphosphate + (deoxyribonucleotide)m+n
-
 ?
6.5.1.1 dATP + (deoxyribonucleotide)n + (deoxyribonucleotide)m very low activity Saccharolobus shibatae dAMP + diphosphate + (deoxyribonucleotide)m+n
-
 ?

Subunits

EC Number Subunits Comment Organism
6.5.1.1 monomer 1 * 67647, deduced from nucleotide sequence Saccharolobus shibatae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.5.1.1 60 80
-
 Saccharolobus shibatae

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
6.5.1.1 45 90
-
 Saccharolobus shibatae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.5.1.1 80
-
 15% loss of activity after 5 h Saccharolobus shibatae
6.5.1.1 85
-
 29% loss of activity after 5 h Saccharolobus shibatae
6.5.1.1 90
-
 50% loss of activity after approx. 10 min Saccharolobus shibatae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5.1.1 6 7
-
 Saccharolobus shibatae

pI Value

EC Number Organism Comment pI Value Maximum pI Value
6.5.1.1 Saccharolobus shibatae deduced from nucleotide sequence
-
 5.86