BRENDA - Enzyme Database

Purification and characterization of a cold-adapted isocitrate lyase and expression analysis of the cold-inducible isocitrate lyase gene from the psychrophilic bacterium Colwellia psychrerythraea

Watanabe, S.; Yamaoka, N.; Fukunaga, N.; Takada, Y.; Extremophiles 6, 397-405 (2002)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.633
-
DL-isocitrate
15°C, pH 6.8, Km increases with elevating temperature
Colwellia maris
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.1
Co2+
can replace Mg2+
Colwellia maris
4.1.3.1
Mg2+
10 mM, dependent
Colwellia maris
4.1.3.1
Mn2+
can partly replace Mg2+
Colwellia maris
4.1.3.1
Ni2+
can partly replace Mg2+
Colwellia maris
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
64000
-
4 * 64000, SDS-PAGE
Colwellia maris
4.1.3.1
240000
-
gel-filtration
Colwellia maris
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Colwellia maris
-
succinate + glyoxylate
-
Colwellia maris
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.3.1
Colwellia maris
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.1.3.1
-
Colwellia maris
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.3.1
4.52
-
-
Colwellia maris
Storage Stability
EC Number
Storage Stability
Organism
4.1.3.1
-35°C, more than 80% of the activity remains after more than 1 year
Colwellia maris
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
651215
Colwellia maris
succinate + glyoxylate
-
651215
Colwellia maris
?
Subunits
EC Number
Subunits
Commentary
Organism
4.1.3.1
tetramer
4 * 64000, SDS-PAGE
Colwellia maris
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.1.3.1
25
-
-
Colwellia maris
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.1.3.1
30
-
complete inactivation after 2 min
Colwellia maris
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.633
-
DL-isocitrate
15°C, pH 6.8, Km increases with elevating temperature
Colwellia maris
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.1
Co2+
can replace Mg2+
Colwellia maris
4.1.3.1
Mg2+
10 mM, dependent
Colwellia maris
4.1.3.1
Mn2+
can partly replace Mg2+
Colwellia maris
4.1.3.1
Ni2+
can partly replace Mg2+
Colwellia maris
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
64000
-
4 * 64000, SDS-PAGE
Colwellia maris
4.1.3.1
240000
-
gel-filtration
Colwellia maris
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Colwellia maris
-
succinate + glyoxylate
-
Colwellia maris
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.1
-
Colwellia maris
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.3.1
4.52
-
-
Colwellia maris
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
4.1.3.1
-35°C, more than 80% of the activity remains after more than 1 year
Colwellia maris
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
651215
Colwellia maris
succinate + glyoxylate
-
651215
Colwellia maris
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.1.3.1
tetramer
4 * 64000, SDS-PAGE
Colwellia maris
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.1.3.1
25
-
-
Colwellia maris
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.1.3.1
30
-
complete inactivation after 2 min
Colwellia maris