BRENDA - Enzyme Database

Characterization of the pulmonary N-ethylmaleimide-insensitive phosphatidate phosphohydrolase

Nanjundan, M.; Possmayer, F.; Exp. Lung Res. 26, 361-381 (2000)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
Ca2+
10 mM, 60% inhibition of PAP-2
Rattus norvegicus
3.1.3.4
ceramide 1-phosphate
-
Rattus norvegicus
3.1.3.4
chlorpromazine
55% inhibition at equimolar concentration of substrate, PAP-2
Rattus norvegicus
3.1.3.4
diacylglycerol
-
Rattus norvegicus
3.1.3.4
F-
PAP-2
Rattus norvegicus
3.1.3.4
lysophosphatidic acid
-
Rattus norvegicus
3.1.3.4
Mg2+
15 mM MgCl2, 10-20% inhibition of PAP-2
Rattus norvegicus
3.1.3.4
monoacylglycerol
-
Rattus norvegicus
3.1.3.4
NEM
2 mM, complete inhibition of PAP-1. NEM, up to 8 mM has little effect on PAP-2
Rattus norvegicus
3.1.3.4
spermidine trihydrochloride
7% inhibition at equimolar concentration of substrate, PAP-2
Rattus norvegicus
3.1.3.4
spermine
55% inhibition at equimolar concentration of substrate, PAP-2
Rattus norvegicus
3.1.3.4
sphingomyelin
72% inhibition at equimolar concentration with pure phosphatidic acid. No effect with equimolar concentrations of phosphatidic acid mixed with phosphatidylcholine
Rattus norvegicus
3.1.3.4
sphingosine
93% inhibition at equimolar concentration with pure phosphatidic acid. 29% inhibition with equimolar concentrations of phosphatidic acid mixed with phosphatidylcholine
Rattus norvegicus
3.1.3.4
sphingosine 1-phosphate
-
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
plasma membrane
-
Rattus norvegicus
5886
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.4
Mg2+
PAP-1 is dependent on
Rattus norvegicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.3.4
additional information
Rattus norvegicus
key enzyme involved in glycerolipid synthesis where it converts phosphatidic acid to diacylglycerol. PAP-1 is involved in phospholipid biosynthesis
?
-
Rattus norvegicus
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.4
Rattus norvegicus
-
2 different phosphatidate phosphatidases: PAP-1 and PAP-2
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.4
alveolar cell type II
PAP-2
Rattus norvegicus
-
3.1.3.4
lung
PAP-2
Rattus norvegicus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
lysophosphatidic acid + H2O
-
651209
Rattus norvegicus
?
-
651209
Rattus norvegicus
?
3.1.3.4
additional information
key enzyme involved in glycerolipid synthesis where it converts phosphatidic acid to diacylglycerol. PAP-1 is involved in phospholipid biosynthesis
651209
Rattus norvegicus
?
-
651209
Rattus norvegicus
?
3.1.3.4
phosphatidic acid + H2O
phosphatidic acid pure or in mixed micelles with phosphatidylcholine
651209
Rattus norvegicus
1,2-diacyl-sn-glycerol + phosphate
-
651209
Rattus norvegicus
?
3.1.3.4
sphingosine 1-phosphate + H2O
poor substrate
651209
Rattus norvegicus
?
-
651209
Rattus norvegicus
?
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.3.4
55
-
activity of PAP-2 is not seriously affected after 20 min
Rattus norvegicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.4
6
-
phosphatidic acid mixed with phosphatidylcholine, PAP-2
Rattus norvegicus
3.1.3.4
6.5
-
pure phosphatidic acid, PAP-2
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
Ca2+
10 mM, 60% inhibition of PAP-2
Rattus norvegicus
3.1.3.4
ceramide 1-phosphate
-
Rattus norvegicus
3.1.3.4
chlorpromazine
55% inhibition at equimolar concentration of substrate, PAP-2
Rattus norvegicus
3.1.3.4
diacylglycerol
-
Rattus norvegicus
3.1.3.4
F-
PAP-2
Rattus norvegicus
3.1.3.4
lysophosphatidic acid
-
Rattus norvegicus
3.1.3.4
Mg2+
15 mM MgCl2, 10-20% inhibition of PAP-2
Rattus norvegicus
3.1.3.4
monoacylglycerol
-
Rattus norvegicus
3.1.3.4
NEM
2 mM, complete inhibition of PAP-1. NEM, up to 8 mM has little effect on PAP-2
Rattus norvegicus
3.1.3.4
spermidine trihydrochloride
7% inhibition at equimolar concentration of substrate, PAP-2
Rattus norvegicus
3.1.3.4
spermine
55% inhibition at equimolar concentration of substrate, PAP-2
Rattus norvegicus
3.1.3.4
sphingomyelin
72% inhibition at equimolar concentration with pure phosphatidic acid. No effect with equimolar concentrations of phosphatidic acid mixed with phosphatidylcholine
Rattus norvegicus
3.1.3.4
sphingosine
93% inhibition at equimolar concentration with pure phosphatidic acid. 29% inhibition with equimolar concentrations of phosphatidic acid mixed with phosphatidylcholine
Rattus norvegicus
3.1.3.4
sphingosine 1-phosphate
-
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
plasma membrane
-
Rattus norvegicus
5886
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.4
Mg2+
PAP-1 is dependent on
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.3.4
additional information
Rattus norvegicus
key enzyme involved in glycerolipid synthesis where it converts phosphatidic acid to diacylglycerol. PAP-1 is involved in phospholipid biosynthesis
?
-
Rattus norvegicus
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.4
alveolar cell type II
PAP-2
Rattus norvegicus
-
3.1.3.4
lung
PAP-2
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
lysophosphatidic acid + H2O
-
651209
Rattus norvegicus
?
-
651209
Rattus norvegicus
?
3.1.3.4
additional information
key enzyme involved in glycerolipid synthesis where it converts phosphatidic acid to diacylglycerol. PAP-1 is involved in phospholipid biosynthesis
651209
Rattus norvegicus
?
-
651209
Rattus norvegicus
?
3.1.3.4
phosphatidic acid + H2O
phosphatidic acid pure or in mixed micelles with phosphatidylcholine
651209
Rattus norvegicus
1,2-diacyl-sn-glycerol + phosphate
-
651209
Rattus norvegicus
?
3.1.3.4
sphingosine 1-phosphate + H2O
poor substrate
651209
Rattus norvegicus
?
-
651209
Rattus norvegicus
?
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.1.3.4
55
-
activity of PAP-2 is not seriously affected after 20 min
Rattus norvegicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.4
6
-
phosphatidic acid mixed with phosphatidylcholine, PAP-2
Rattus norvegicus
3.1.3.4
6.5
-
pure phosphatidic acid, PAP-2
Rattus norvegicus