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Literature summary extracted from
- Activation of recombinant bovine matrix metalloproteinase-20 and its hydrolysis of two amelogenin oligopeptides (1999), Eur. J. Oral Sci., 107, 352-359.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.24.B6 | overexpression of His-atgged pro-enzyme in Escherichia coli BL21(DE3) | Bos taurus |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.24.B6 | extracellular | in enamel matrix of teeth | Bos taurus | - |
- |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.24.B6 | amylogenin + H2O | Bos taurus | involved in tooth formation | ? | - |
? |  |
| 3.4.24.B6 | proteins + H2O | Bos taurus | - |
peptides | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.24.B6 | Bos taurus | O18767 | - |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.4.24.B6 | proteolytic modification | sequential autolysis, activation of recombinant proform by urea | Bos taurus |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.24.B6 | recombinant His-tagged pro-enzyme from Escherichia coli | Bos taurus |
Renatured (Commentary)
| EC Number | Renatured (Comment) | Organism |
|---|---|---|
| 3.4.24.B6 | renaturation from inclusion bodies after overexpression in Escherichia coli | Bos taurus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.24.B6 | ameloblast | secretion | Bos taurus | - |
| 3.4.24.B6 | tooth | - |
Bos taurus | - |
| 3.4.24.B6 | tooth enamel | - |
Bos taurus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.24.B6 | amylogenin + H2O | - |
Bos taurus | ? | - |
? | |
| 3.4.24.B6 | amylogenin + H2O | involved in tooth formation | Bos taurus | ? | - |
? | |
| 3.4.24.B6 | casein + H2O | - |
Bos taurus | ? | - |
? | |
| 3.4.24.B6 | leucine-rich amylogenin peptide + H2O | corresponds to residues 181-188 of amylogenin, recombinant enzyme, cleavage site is P186-A187 | Bos taurus | leucine-rich amylogenin residues 181-186 + leucine-rich amylogenin residues 187-188 | - |
? | |
| 3.4.24.B6 | proteins + H2O | - |
Bos taurus | peptides | - |
? | |
| 3.4.24.B6 | tyrosine-rich amylogenin peptide + H2O | corresponds to residues 36-49 of amylogenin, recombinant enzyme, cleavage site is W45-L46 | Bos taurus | tyrosine-rich amylogenin residues 36-45 + tyrosine-rich amylogenin residues 46-49 | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.24.B6 | enamel metalloproteinase | - |
Bos taurus |
| 3.4.24.B6 | enamelysin | - |
Bos taurus |
| 3.4.24.B6 | M10.019 | Merops-ID | Bos taurus |
| 3.4.24.B6 | MMP-20 | - |
Bos taurus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.B6 | 37 | - |
assay at, zymography with casein at room temperature | Bos taurus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.24.B6 | 7.5 | - |
assay at | Bos taurus |
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Release 2023.1 (January 2023)
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