EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.24.B11 | expression of His-tagged enzyme in insect cells via baculovirus infection | Homo sapiens |
3.4.24.B12 | correction of point mutations G577S and Q579R by overlap extension PCR, subcloning from FLAG-tagged construct in the baculovirus, cloning and expression of His-FLAG-tagged wild-type enzyme and mutants in insect cells via baculovirus infection | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.B11 | catechin gallate esters | from green tea Camellia sinensis, strong inhibition, independent of Zn2+, reversible | Homo sapiens | |
3.4.24.B12 | catechin gallate esters | from green tea Camellia sinensis, strong inhibition, independent of Zn2+, reversible | Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.4.24.B11 | extracellular | - |
Homo sapiens | - |
- |
3.4.24.B12 | extracellular | - |
Homo sapiens | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.B11 | Zn2+ | metalloprotease | Homo sapiens | |
3.4.24.B12 | Zn2+ | metalloprotease | Homo sapiens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.24.B11 | 89000 | - |
x * 89000, recombinant mature enzyme, SDS-PAGE | Homo sapiens |
3.4.24.B12 | 64000 | - |
x * 64000, C-terminally processed recombinant mature enzyme, SDS-PAGE | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.24.B11 | proteins + H2O | Homo sapiens | - |
peptides | - |
? | |
3.4.24.B12 | proteins + H2O | Homo sapiens | - |
peptides | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.24.B11 | Homo sapiens | Q9UHI8 | - |
- |
3.4.24.B12 | Homo sapiens | Q9UNA0 | - |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.4.24.B11 | proteolytic modification | recombinant enzyme is also processed in insect cells | Homo sapiens |
3.4.24.B12 | proteolytic modification | recombinant enzyme is also processed in insect cells | Homo sapiens |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.24.B11 | partially, recombinant His-tagged enzyme from insect cells, 200fold | Homo sapiens |
3.4.24.B12 | partially, recombinant His-tagged enzyme from insect cells, 185fold | Homo sapiens |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.4.24.B11 | proteolytic degradation of proteins | strict specificity for cleavage between glutamic acid and uncharged aliphatic amino acids in the core proteins of large aggregating proteoglycans | Homo sapiens | |
3.4.24.B12 | proteolytic degradation of proteins | strict specificity for cleavage between glutamic acid and uncharged aliphatic amino acids in the core proteins of large aggregating proteoglycans | Homo sapiens |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.4.24.B11 | 140 | - |
partially purified recombinant enzyme | Homo sapiens |
3.4.24.B12 | 1200 | - |
partially purified recombinant enzyme | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.24.B11 | aggrecan + H2O | several cleavage sites are determined | Homo sapiens | 11 aggrecan fragment | product determination, overview | ? | |
3.4.24.B11 | proteins + H2O | - |
Homo sapiens | peptides | - |
? | |
3.4.24.B11 | proteins + H2O | strict specificity for cleavage between glutamic acid and uncharged aliphatic amino acids in the core proteins of large aggregating proteoglycans | Homo sapiens | peptides | - |
? | |
3.4.24.B12 | aggrecan + H2O | several cleavage sites are determined | Homo sapiens | 7 aggregan fragment | product determination, overview | ? | |
3.4.24.B12 | proteins + H2O | - |
Homo sapiens | peptides | - |
? | |
3.4.24.B12 | proteins + H2O | strict specificity for cleavage between glutamic acid and uncharged aliphatic amino acids in the core proteins of large aggregating proteoglycans | Homo sapiens | peptides | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.24.B11 | ? | x * 89000, recombinant mature enzyme, SDS-PAGE | Homo sapiens |
3.4.24.B12 | ? | x * 64000, C-terminally processed recombinant mature enzyme, SDS-PAGE | Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.24.B11 | ADAMTS1 | - |
Homo sapiens |
3.4.24.B11 | M12.222 | Merops-ID | Homo sapiens |
3.4.24.B12 | ADAMTS5 | - |
Homo sapiens |
3.4.24.B12 | M12.225 | Merops-ID | Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.24.B11 | 37 | - |
assay at | Homo sapiens |
3.4.24.B12 | 37 | - |
assay at | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.24.B11 | 7.5 | - |
assay at | Homo sapiens |
3.4.24.B12 | 7.5 | - |
assay at | Homo sapiens |