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Literature summary extracted from

  • Snellman, E.A.; Sullivan, E.R.; Colwell, R.R.
    Purification and properties of the extracellular lipase, LipA, of Acinetobacter sp. RAG-1 (2002), Eur. J. Biochem., 269, 5771-5779.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.3 Ca2+
-
 Acinetobacter sp.
3.1.1.3 Co2+
-
 Acinetobacter sp.
3.1.1.3 Fe3+
-
 Acinetobacter sp.
3.1.1.3 Mg2+ slight activation Acinetobacter sp.
3.1.1.3 Mn2+ slight activation Acinetobacter sp.
3.1.1.3 Rb+
-
 Acinetobacter sp.

General Stability

EC Number General Stability Organism
3.1.1.3 Ca2+ stabilizes the enzyme, no loss of activity after 30 h at 30°C in presence of Ca2+, 70% loss after 30 h at 30°C of activity in absence of Ca2+ Acinetobacter sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.3 Cu2+ strong inhibition Acinetobacter sp.
3.1.1.3 EDTA strong, about 90% inhibition at 1 mM Acinetobacter sp.
3.1.1.3 Hg2+ strong inhibition Acinetobacter sp.
3.1.1.3 additional information no inhibition by 2-mercaptoethanol, dithiothreitol, and Zn2+ Acinetobacter sp.
3.1.1.3 PMSF slight inhibition Acinetobacter sp.
3.1.1.3 Pyridine rapid deactivation Acinetobacter sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.3 extracellular LipA, secreted to the medium Acinetobacter sp.
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 33000
-
 x * 33000, SDS-PAGE Acinetobacter sp.

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.1.1.3 acetonitrile loss of about 25% activity at 30% v/v after 1 h at 30°C Acinetobacter sp.
3.1.1.3 dimethylformamide loss of about 10% activity at 30% v/v after 1 h at 30°C Acinetobacter sp.
3.1.1.3 additional information the enzyme is only very slightly affected by isopropanol and acetone Acinetobacter sp.
3.1.1.3 Pyridine rapid complete deactivation Acinetobacter sp.
3.1.1.3 tetrahydrofuran loss of about 90% activity at 30% v/v after 1 h at 30°C Acinetobacter sp.

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Acinetobacter sp.
-
 LipA
-
3.1.1.3 Acinetobacter sp. RAG-1
-
 LipA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 from culture medium, 10.7fold Acinetobacter sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 412
-
 purified enzyme Acinetobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 4-nitrophenyl acetate + H2O low activity Acinetobacter sp. 4-nitrophenol + acetate
-
 ?
3.1.1.3 4-nitrophenyl acetate + H2O low activity Acinetobacter sp. RAG-1 4-nitrophenol + acetate
-
 ?
3.1.1.3 4-nitrophenyl butyrate + H2O
-
 Acinetobacter sp. 4-nitrophenol + butyrate
-
 ?
3.1.1.3 4-nitrophenyl butyrate + H2O
-
 Acinetobacter sp. RAG-1 4-nitrophenol + butyrate
-
 ?
3.1.1.3 4-nitrophenyl decanoate + H2O
-
 Acinetobacter sp. 4-nitrophenol + decanoate
-
 ?
3.1.1.3 4-nitrophenyl hexanoate + H2O best substrate Acinetobacter sp. 4-nitrophenol + hexanoate
-
 ?
3.1.1.3 4-nitrophenyl hexanoate + H2O best substrate Acinetobacter sp. RAG-1 4-nitrophenol + hexanoate
-
 ?
3.1.1.3 4-nitrophenyl laurate + H2O
-
 Acinetobacter sp. 4-nitrophenol + laurate
-
 ?
3.1.1.3 4-nitrophenyl myristate + H2O
-
 Acinetobacter sp. 4-nitrophenol + myristate
-
 ?
3.1.1.3 4-nitrophenyl octanoate + H2O best substrate Acinetobacter sp. 4-nitrophenol + octanoate
-
 ?
3.1.1.3 4-nitrophenyl octanoate + H2O best substrate Acinetobacter sp. RAG-1 4-nitrophenol + octanoate
-
 ?
3.1.1.3 4-nitrophenyl palmitate + H2O
-
 Acinetobacter sp. 4-nitrophenol + palmitate
-
 ?
3.1.1.3 4-nitrophenyl stearate + H2O
-
 Acinetobacter sp. 4-nitrophenol + stearate
-
 ?
3.1.1.3 additional information substrate specificity, enzyme prefers substrates of medium- and long-chain fatty acids Acinetobacter sp. ?
-
 ?
3.1.1.3 additional information substrate specificity, enzyme prefers substrates of medium- and long-chain fatty acids Acinetobacter sp. RAG-1 ?
-
 ?
3.1.1.3 tributyrin + H2O
-
 Acinetobacter sp. dibutyrin + butyrate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 ? x * 33000, SDS-PAGE Acinetobacter sp.

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 LipA
-
 Acinetobacter sp.
3.1.1.3 lipase
-
 Acinetobacter sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 55
-
-
 Acinetobacter sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.3 30 70 33% of maximal activity at 30°C, about 50% of maximal activity at 70°C Acinetobacter sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 70
-
 active up to Acinetobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 9
-
-
 Acinetobacter sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.3 8 10 high activity Acinetobacter sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.3 Acinetobacter sp. isoelectric focusing
-
 5.9