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Literature summary extracted from

  • Yamanishi, M.; Yunoki, M.; Tobimatsu, T.; Sato, H.; Matsui, J.; Dokiya, A.; Iuchi, Y.; Oe, K.; Suto, K.; Shibata, N.; Morimoto, Y.; Yasuoka, N.; Toraya, T.
    The crystal structure of coenzyme B12-dependent glycerol dehydratase in complex with cobalamin and propane-1,2-diol (2002), Eur. J. Biochem., 269, 4484-4494.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.30 adenosylcobalamin bound at the interface between the alpha and beta subunits Klebsiella pneumoniae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.1.30 complexed with cyanocobalamin an propane-1,2-diol, sandwich-drop vapor diffusion method Klebsiella pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.30 0.32
-
 Propane-1,2-diol 37°C, recombinant enzyme, purification method 1 and 2 Klebsiella pneumoniae
4.2.1.30 0.36
-
 Propane-1,2-diol 37°C Klebsiella pneumoniae
4.2.1.30 1.1
-
 glycerol 37°C, recombinant enzyme, purification method 2 Klebsiella pneumoniae
4.2.1.30 1.2
-
 glycerol 37°C, recombinant enzyme, purification method 1 Klebsiella pneumoniae
4.2.1.30 1.5
-
 glycerol 37°C Klebsiella pneumoniae
4.2.1.30 8
-
 adenosylcobalamin 37°C, recombinant enzyme, purification method 1 and 2 Klebsiella pneumoniae
4.2.1.30 20
-
 adenosylcobalamin 37°C Klebsiella pneumoniae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.30 K+ essential, bound inside the(beta/alpha)8 barrel above the corrin ring of cobalamin Klebsiella pneumoniae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.30 16000
-
 gamma-subunit, SDS-PAGE Klebsiella pneumoniae
4.2.1.30 22000
-
 beta-subunit, SDS-PAGE Klebsiella pneumoniae
4.2.1.30 61000
-
 alpha-subunit, SDS-PAGE Klebsiella pneumoniae
4.2.1.30 200000
-
 native PAGE, 2 bands: 200000 Da and 220000 Da Klebsiella pneumoniae
4.2.1.30 220000
-
 native PAGE, 2 bands: 200000 Da and 220000 Da Klebsiella pneumoniae

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.30 Klebsiella pneumoniae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.30 homogeneity, recombinant enzyme, 2 different purification methods Klebsiella pneumoniae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.30 65.4 120 dependent on purification method Klebsiella pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.30 glycerol
-
 Klebsiella pneumoniae 3-hydroxypropanal + H2O
-
 ?
4.2.1.30 propane-1,2-diol (R)-enantiomer is used 2.5 times faster than the (S)-isomer Klebsiella pneumoniae ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.30 hexamer alpha2beta2gamma2, native PAGE and SDS-PAGE Klebsiella pneumoniae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.30 392
-
 Propane-1,2-diol 37°C, recombinant enzyme, purification method 2 Klebsiella pneumoniae
4.2.1.30 860
-
 glycerol 37°C, recombinant enzyme, purification method 2 Klebsiella pneumoniae