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Literature summary extracted from

  • Schmid, M.; Vorburger, T.; Pos, K.M.; Dimroth, P.
    Role of conserved residues within helices IV and VIII of the oxaloacetate decarboxylase beta subunit in the energy coupling mechanism of the Na+ pump (2002), Eur. J. Biochem., 269, 2997-3004.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.112 expressed in Escherichia coli Klebsiella pneumoniae

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.112 N373D beta subunit, no protection against tryptic digestion by Na+ Klebsiella pneumoniae
4.1.1.112 N373L beta subunit, very low activity, no protection against tryptic digestion by Na+ Klebsiella pneumoniae
4.1.1.112 R389A beta subunit, significantly increased pH-optimum Klebsiella pneumoniae
4.1.1.112 R389D beta subunit, very low activity Klebsiella pneumoniae
4.1.1.112 S382A beta subunit, no activity below 20 mM Na+, active with 400 mM NaCl, no protection against tryptic digestion by Na+, no Na+ transport Klebsiella pneumoniae
4.1.1.112 S382D beta subunit, very low activity Klebsiella pneumoniae
4.1.1.112 S382T beta subunit, very low activity Klebsiella pneumoniae

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.112 NaCl almost complete inhibition at 0.4 M Klebsiella pneumoniae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.1.112 membrane beta and gamma subunits are integral membrane proteins, alpha subunit is attached to the gamma subunit Klebsiella pneumoniae 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.112 Na+ protects against tryptic digestion Klebsiella pneumoniae
4.1.1.112 Zn2+ binds to the gamma subunit Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.112 Oxaloacetate Klebsiella pneumoniae two Na+ are transported into the periplasm and one H+ is transported into the cytoplasm during the reaction Pyruvate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.112 Klebsiella pneumoniae
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.112 recombinant enzyme from Escherichia coli Klebsiella pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.112 Oxaloacetate
-
 Klebsiella pneumoniae Pyruvate + CO2
-
 ?
4.1.1.112 Oxaloacetate two Na+ are transported into the periplasm and one H+ is transported into the cytoplasm during the reaction Klebsiella pneumoniae Pyruvate + CO2
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.112 trimer alpha, beta, gamma Klebsiella pneumoniae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.112 6.25 6.75 wild type enzyme Klebsiella pneumoniae
4.1.1.112 7
-
 pH optimum at about pH 7 for all mutant enzymes except R389A Klebsiella pneumoniae
4.1.1.112 9.2
-
 R389A mutant Klebsiella pneumoniae

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.112 biotin binds to the C-terminal domain of the alpha-subunit Klebsiella pneumoniae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1.1.112 additional information
-
 additional information Ki-values given for inhibition with NaCl for all mutant enzymes at 3-4 different pH Klebsiella pneumoniae
4.1.1.112 198
-
 NaCl wild type enzyme, pH 6.9 Klebsiella pneumoniae