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Literature summary extracted from

  • Laustsen, P.G.; Vang, S.; Kristensen, T.
    Mutational analysis of the active site of human insulin-regulated aminopeptidase (2001), Eur. J. Biochem., 268, 98-104.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.3 expression in HEK293 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.4.11.3 A429G the ratio of maximal velocity to Km-value is 75% of the value for the wild-type enzyme Homo sapiens
3.4.11.3 E431A strong decrease in aminopeptidase activity towards Leu-4-nitroanilide Homo sapiens
3.4.11.3 E431D strong decrease in aminopeptidase activity towards Leu-4-nitroanilide Homo sapiens
3.4.11.3 E465D strong decrease in aminopeptidase activity towards Leu-4-nitroanilide Homo sapiens
3.4.11.3 E465Q strong decrease in aminopeptidase activity towards Leu-4-nitroanilide Homo sapiens
3.4.11.3 E487D strong decrease in aminopeptidase activity towards Leu-4-nitroanilide Homo sapiens
3.4.11.3 E487Q inactive mutant enzyme Homo sapiens
3.4.11.3 G428A the ratio of maximal velocity to Km-value is 90% of the value for the wild-type enzyme Homo sapiens
3.4.11.3 H464Y strong decrease in aminopeptidase activity towards Leu-4-nitroanilide Homo sapiens
3.4.11.3 H468Y strong decrease in aminopeptidase activity towards Leu-4-nitroanilide Homo sapiens
3.4.11.3 M430E strong decrease in aminopeptidase activity towards Leu-4-nitroanilide Homo sapiens
3.4.11.3 M430I the ratio of maximal velocity to Km-value is 6.2% of the value for the wild-type enzyme Homo sapiens
3.4.11.3 M430K the ratio of maximal velocity to Km-value is 3.3% of the value for the wild-type enzyme Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.3 0.243
-
 Leu-4-nitroanilide pH 7.4, 37°C, mutant enzyme A429G Homo sapiens
3.4.11.3 0.331
-
 Cys-4-nitroanilide pH 7.4, 37°C, wild-type enzyme Homo sapiens
3.4.11.3 0.4567
-
 Leu-4-nitroanilide pH 7.4, 37°C, mutant enzyme M430I Homo sapiens
3.4.11.3 0.488
-
 Leu-4-nitroanilide pH 7.4, 37°C, wild-type enzyme Homo sapiens
3.4.11.3 0.529
-
 Arg-4-nitroanilide pH 7.4, 37°C, wild-type enzyme Homo sapiens
3.4.11.3 0.593
-
 Leu-4-nitroanilide pH 7.4, 37°C, mutant enzyme G428A Homo sapiens
3.4.11.3 0.9148
-
 Leu-4-nitroanilide pH 7.4, 37°C, mutant enzyme M430K Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.3 membrane
-
 Homo sapiens 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.3 Zinc contains the characteristic Zn2+-coordination sequence element, HEXXH-(18-64X)-E Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.3 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.3 Arg-4-nitroanilide + H2O
-
 Homo sapiens Arg + 4-nitroaniline
-
 ?
3.4.11.3 Cys-4-nitroanilide + H2O
-
 Homo sapiens Cys + 4-nitroaniline
-
 ?
3.4.11.3 Leu-4-nitroanilide + H2O
-
 Homo sapiens Leu + 4-nitroaniline
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.11.3 insulin-regulated aminopeptidase
-
 Homo sapiens
3.4.11.3 IRAP
-
 Homo sapiens
3.4.11.3 oxytocinase
-
 Homo sapiens