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Literature summary extracted from

  • Brock, M.; Darley, D.; Textor, S.; Buckel, W.
    2-Methylisocitrate lyases from the bacterium Escherichia coli and the filamentous fungus Aspergillus nidulans: characterization and comparison of both enzymes (2001), Eur. J. Biochem., 268, 3577-3586.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.1.3.30 enzyme becomes inactivated when incubated with oxygen, partial reactivation with dithiothreitol possible Aspergillus nidulans

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.30 3-Bromopyruvate complete inactivation at equimolar concentrations Aspergillus nidulans
4.1.3.30 Ca2+ 40% inactivation at 2 mM; inhibition at 2 mM Aspergillus nidulans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.30 19
-
 (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate pH 7 Aspergillus nidulans
4.1.3.30 31
-
 (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate pH 7 Aspergillus nidulans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.30 Mg2+ dependent, 2 mM, Km: 58 mM at pH 7 Aspergillus nidulans
4.1.3.30 Mg2+ dependentm Km: 35 mM at pH 7 Aspergillus nidulans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.30 32000
-
 4 * 32000, SDS-PAGE Aspergillus nidulans
4.1.3.30 66000
-
 4 * 66000, SDS-PAGE Aspergillus nidulans
4.1.3.30 130000
-
 gel filtration Aspergillus nidulans
4.1.3.30 244000
-
 gel filtration Aspergillus nidulans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.3.30 additional information Aspergillus nidulans enzyme in methylcitrate cycle ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.30 Aspergillus nidulans
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
4.1.3.30 enzyme becomes inactivated when incubated with oxygen, partial reactivation with dithiothreitol possible Aspergillus nidulans

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.30
-
 Aspergillus nidulans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.3.30 21.4
-
-
 Aspergillus nidulans
4.1.3.30 91
-
-
 Aspergillus nidulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.30 (2S,3R)-3-Hydroxybutane-1,2,3-tricarboxylate syn: methylisocitrate, enzyme acts only on threo-diastereomer, but not on the erythro-diastereomer Aspergillus nidulans Pyruvate + succinate
-
 ?
4.1.3.30 additional information no substrate: isocitrate Aspergillus nidulans ?
-
 ?
4.1.3.30 additional information enzyme in methylcitrate cycle Aspergillus nidulans ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.3.30 tetramer 4 * 66000, SDS-PAGE Aspergillus nidulans
4.1.3.30 tetramer 4 * 32000, SDS-PAGE Aspergillus nidulans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.3.30
-
-
 stable for several days Aspergillus nidulans
4.1.3.30 40
-
 stable for 60 min Aspergillus nidulans
4.1.3.30 50
-
 half-life: 15 min Aspergillus nidulans
4.1.3.30 50
-
 complete inactivation after 15 min Aspergillus nidulans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.3.30 12.3
-
 (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate pH 7 Aspergillus nidulans
4.1.3.30 100
-
 (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate pH 7 Aspergillus nidulans