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Literature summary extracted from

  • Vankemmelbeke, M.N.; Holen, I.; Wilson, A.G.; Ilic, M.Z.; Handley, C.J.; Kelner, G.S.; Clark, M.; Liu, C.; Maki, R.A.; Burnett, D.; Buttle, D.J.
    Expression and activity of ADAMTS-5 in synovium (2001), Eur. J. Biochem., 268, 1259-1268.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.B12 expression of FLAG-tagged enzyme in Spodoptera frugiperda Sf9 cells via baculovirus infection, secretion of recombinant protein to the medium Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.B12 heparin
-
 Bos taurus
3.4.24.B12 heparin
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.24.B12 extracellular
-
 Homo sapiens
-
-
3.4.24.B12 extracellular
-
 Bos taurus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.B12 60000
-
 x * 60000, SDS-PAGE Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.B12 aggrecan + H2O Homo sapiens enzyme may play a role in cartilage breakdown ?
-
 ?
3.4.24.B12 aggrecan + H2O Bos taurus enzyme may play a role in cartilage breakdown ?
-
 ?
3.4.24.B12 proteins + H2O Homo sapiens
-
 peptides
-
 ?
3.4.24.B12 proteins + H2O Bos taurus
-
 peptides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.B12 Bos taurus Q9TT92
-
-
3.4.24.B12 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.24.B12 proteolytic modification cleavage by furin Homo sapiens
3.4.24.B12 proteolytic modification cleavage by furin Bos taurus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.B12 recombinant FLAG-tagged from insect Sf9 cells Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.B12 cartilage
-
 Homo sapiens
-
3.4.24.B12 synovial membrane
-
 Bos taurus
-
3.4.24.B12 synovium
-
 Bos taurus
-
3.4.24.B12 synovium arthritic Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.B12 aggrecan + H2O substrate from cartilage, extensive digestion, N-terminal cleavage sites between glutamate and small uncharged aliphatic amino acids in nonglycosylated regions of the aggregan core protein: the interglobular domain E373-A, and between chondroitin sulfate attachment sites 1 and 2 E1480-G Homo sapiens ?
-
 ?
3.4.24.B12 aggrecan + H2O substrate from cartilage, N-terminal cleavage sites between glutamate and small uncharged aliphatic amino acids in nonglycosylated regions of the aggregan core protein: the interglobular domain E373-A, and within the chondroitinsulfate 2 attachment region E1666-G, E1771-A, and E1871-L Bos taurus ?
-
 ?
3.4.24.B12 aggrecan + H2O enzyme may play a role in cartilage breakdown Homo sapiens ?
-
 ?
3.4.24.B12 aggrecan + H2O enzyme may play a role in cartilage breakdown Bos taurus ?
-
 ?
3.4.24.B12 additional information interaction with sulfated glucosaminglycan may be very important for the localization and activity of the enzyme Bos taurus ?
-
 ?
3.4.24.B12 additional information interaction with sulfated glucosaminglycan may be very important for the localization and activity of the enzymew Homo sapiens ?
-
 ?
3.4.24.B12 proteins + H2O
-
 Homo sapiens peptides
-
 ?
3.4.24.B12 proteins + H2O
-
 Bos taurus peptides
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.24.B12 ? x * 60000, SDS-PAGE Bos taurus

Synonyms

EC Number Synonyms Comment Organism
3.4.24.B12 ADAMTS-5
-
 Homo sapiens
3.4.24.B12 ADAMTS-5
-
 Bos taurus
3.4.24.B12 aggrecanase-2
-
 Homo sapiens
3.4.24.B12 aggrecanase-2
-
 Bos taurus
3.4.24.B12 M12.225 Merops-ID Homo sapiens
3.4.24.B12 M12.225 Merops-ID Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.B12 37
-
 assay at Homo sapiens
3.4.24.B12 37
-
 assay at Bos taurus