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Literature summary extracted from

  • Gunther, R.; Thust, S.; Hofmann, H.J.; Bordusa, F.
    Trypsin-specific acyl-4-guanidinophenyl esters for alpha-chymotrypsin-catalysed reactions computational predictions, hydrolyses, and peptide bond formation (2000), Eur. J. Biochem., 267, 3496-3501.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.1 0.44
-
 benzoyl-4-guanidinophenyl ester 25°C, pH 7.6 Bos taurus
3.4.21.1 1.49
-
 benzoyl-D-Leu-4-guanidinophenyl ester 25°C, pH 7.6 Bos taurus
3.4.21.1 2.58
-
 benzoyl-D-Phe-4-guanidinophenyl ester 25°C, pH 7.6 Bos taurus
3.4.21.1 2.81
-
 benzoyl-Gly-4-guanidinophenyl ester 25°C, pH 7.6 Bos taurus
3.4.21.1 2.88
-
 benzoyl-L-Ala-4-guanidinophenyl ester 25°C, pH 7.6 Bos taurus
3.4.21.1 4.94
-
 benzoyl-D-Ala-4-guanidinophenyl ester 25°C, pH 7.6 Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.1 Bos taurus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.1 benzoyl-4-guanidinophenyl ester + H2O
-
 Bos taurus benzoyl + 4-guanidinophenol
-
 ?
3.4.21.1 benzoyl-D-Ala-4-guanidinophenyl ester + H2O 5% of activity with N-benzoyl-L-Ala-4-guanidinophenyl ester Bos taurus benzoyl-D-Ala + 4-guanidinophenol
-
 ?
3.4.21.1 benzoyl-D-Leu-4-guanidinophenyl ester + H2O
-
 Bos taurus benzoyl-D-Leu + 4-guanidinophenol
-
 ?
3.4.21.1 benzoyl-D-Phe-4-guanidinophenyl ester + H2O
-
 Bos taurus benzoyl-D-Phe + 4-guanidinophenol
-
 ?
3.4.21.1 benzoyl-Gly-4-guanidinophenyl ester + H2O
-
 Bos taurus benzoyl-Gly + 4-guanidinophenol
-
 ?
3.4.21.1 benzoyl-L-Ala-4-guanidinophenyl ester + H2O
-
 Bos taurus benzoyl-L-Ala + 4-guanidinophenol
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.1 additional information
-
 benzoyl-D-Phe-4-guanidinophenyl ester 25°C, pH 7.6 Bos taurus
3.4.21.1 0.13
-
 benzoyl-4-guanidinophenyl ester 25°C, pH 7.6 Bos taurus
3.4.21.1 0.56
-
 benzoyl-D-Ala-4-guanidinophenyl ester 25°C, pH 7.6 Bos taurus
3.4.21.1 1.18
-
 benzoyl-Gly-4-guanidinophenyl ester 25°C, pH 7.6 Bos taurus
3.4.21.1 3 6 benzoyl-D-Leu-4-guanidinophenyl ester 25°C, pH 7.6 Bos taurus
3.4.21.1 3.04
-
 benzoyl-D-Leu-4-guanidinophenyl ester 25°C, pH 7.6 Bos taurus
3.4.21.1 5.54
-
 benzoyl-D-Phe-4-guanidinophenyl ester 25°C, pH 7.6 Bos taurus
3.4.21.1 6.08
-
 benzoyl-Gly-4-guanidinophenyl ester 25°C, pH 7.6 Bos taurus
3.4.21.1 6.08
-
 benzoyl-D-Ala-4-guanidinophenyl ester 25°C, pH 7.6 Bos taurus
3.4.21.1 10.4
-
 benzoyl-L-Ala-4-guanidinophenyl ester 25°C, pH 7.6 Bos taurus