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Literature summary extracted from

  • Elleby, B.; Sjoeblom, B.; Lindskog, S.
    Changing the efficiency and specificity of the esterase activity of human carbonic anhydrase II by site-specific mutagenesis (1999), Eur. J. Biochem., 262, 516-521.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.1 A65L site-specific mutagenesis, enhancement of activity with all substrates, about 5fold increase in activity with 4-nitrophenyl acetate Homo sapiens
4.2.1.1 A65L/T200G site-specific mutagenesis, 5fold increase in activity with 4-nitrophenylacetate Homo sapiens
4.2.1.1 A65L/T200R site-specific mutagenesis, 4fold increase in activity with 4-nitrophenylacetate Homo sapiens
4.2.1.1 L198A site-specific mutagenesis, 5fold decrease in activity with 4-nitrophenylacetate Homo sapiens
4.2.1.1 T200A site-specific mutagenesis, enhancement of activity with all substrates, about 2fold increase in activity with 4-nitrophenyl acetate Homo sapiens
4.2.1.1 T200D site-specific mutagenesis, 93fold decrease in activity with 4-nitrophenylacetate Homo sapiens
4.2.1.1 T200G site-specific mutagenesis, 3fold increase in activity with 4-nitrophenyl acetate Homo sapiens
4.2.1.1 T200H site-specific mutagenesis, enhancement of activity with all substrates, 2fold increase in activity with 4-nitrophenyl acetate Homo sapiens
4.2.1.1 T200I site-specific mutagenesis, enhancement of activity with all substrates, 1.5fold increase in activity with 4-nitrophenyl acetate Homo sapiens
4.2.1.1 T200K site-specific mutagenesis, enhancement of activity with all substrates, 1.3fold increase in activity with 4-nitrophenyl acetate Homo sapiens
4.2.1.1 T200N site-specific mutagenesis, enhancement of activity with all substrates Homo sapiens
4.2.1.1 T200R site-specific mutagenesis, enhancement of activity with all substrates, 7fold increase in activity with 4-nitrophenyl acetate Homo sapiens
4.2.1.1 T200S site-specific mutagenesis, 3fold increase in activity with 4-nitrophenyl acetate, decrease in activity with all other substrates Homo sapiens
4.2.1.1 T200V site-specific mutagenesis, decrease in activity with all substrates Homo sapiens
4.2.1.1 V121A site-specific mutagenesis, 4fold decrease in activity with 4-nitrophenylacetate Homo sapiens
4.2.1.1 V143A site-specific mutagenesis, 2fold decrease in activity with 4-nitrophenylacetate Homo sapiens
4.2.1.1 V143G site-specific mutagenesis, 6fold decrease in activity with 4-nitrophenylacetate Homo sapiens
4.2.1.1 V207A site-specific mutagenesis, 1.5fold decrease in activity with 4-nitrophenylacetate Homo sapiens
4.2.1.1 Y7F site-specific mutagenesis, enhancement of activity with all substrates Homo sapiens
4.2.1.1 Y7F/T200G site-specific mutagenesis, 1.5fold increase in activity with 4-nitrophenylacetate Homo sapiens
4.2.1.1 Y7F/T200R site-specific mutagenesis, 7.5fold increase in activity with 4-nitrophenylacetate Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.1 2-Nitrophenol product inhibition Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.1 H2CO3 Homo sapiens
-
 CO2 + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.1 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.1 2-nitrophenyl acetate + H2O
-
 Homo sapiens 2-nitrophenol + acetate
-
 ?
4.2.1.1 3-nitrophenyl acetate + H2O
-
 Homo sapiens 3-nitrophenol + acetate
-
 ?
4.2.1.1 4-nitrophenyl acetate + H2O
-
 Homo sapiens 4-nitrophenol + acetate
-
 ?
4.2.1.1 4-nitrophenyl propionate + H2O
-
 Homo sapiens 4-nitrophenol + propionate
-
 ?
4.2.1.1 H2CO3
-
 Homo sapiens CO2 + H2O
-
 r

Synonyms

EC Number Synonyms Comment Organism
4.2.1.1 human carbonic anhydrase II
-
 Homo sapiens