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Literature summary extracted from

  • Billecke, S.; Draganov, D.; Counsell, R.; Stetson, P.; Watson, C.; Hsu, C.; La Du, B.N.
    Human serum paraoxonase (PON1) isozymes Q and R hydrolyze lactones and cyclic carbonate esters (2000), Drug Metab. Dispos., 28, 1335-1342.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.2 dilauroyl phosphatidylcholine stimulates activity of enzyme variants Q192 and R192 Homo sapiens
3.1.1.25 dilauroyl phosphatidylcholine stimulates activity of enzyme variants Q192 and R192 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.2 C284A mutant with 44fold reduced paraoxonase activity Homo sapiens
3.1.1.25 C284A no lactonase activity with either homogentisic acid lactone or undecanoic-delta-lactone Homo sapiens
3.1.8.1 C284A mutant with 20fold reduced paraoxonase activity Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.2 Mg2+ activity is strongly reduced when Ca2+ is removed from the purified enzyme and replaced by Zn2+ Homo sapiens
3.1.1.2 Zn2+ activity is strongly reduced when Ca2+ is removed from the purified enzyme and replaced by Zn2+ Homo sapiens
3.1.1.25 Mg2+ activity is strongly reduced when Ca2+ is removed from the purified enzyme and replaced by Mg2+ Homo sapiens
3.1.1.25 Zn2+ activity is strongly reduced when Ca2+ is removed from the purified enzyme and replaced by Zn2+ Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.2 0.27
-
 phenyl acetate enzyme variant R192 Homo sapiens
3.1.1.2 0.7
-
 phenyl acetate enzyme variant Q192 Homo sapiens
3.1.1.25 0.013
-
 dihydrocoumarin 25°C, enzyme variant R192 Homo sapiens
3.1.1.25 0.013
-
 2-coumaranone 25°C, enzyme variant R192 Homo sapiens
3.1.1.25 0.02
-
 2-coumaranone 25°C, enzyme variant Q192 Homo sapiens
3.1.1.25 0.022
-
 dihydrocoumarin 25°C, enzyme variant Q192 Homo sapiens
3.1.1.25 0.45
-
 Homogentisic acid lactone 25°C, enzyme variant R192 Homo sapiens
3.1.1.25 0.52
-
 Homogentisic acid lactone 25°C, enzyme variant Q192 Homo sapiens
3.1.1.25 2.8
-
 gamma-thiobutyrolactone 25°C, enzyme variant Q192 Homo sapiens
3.1.1.25 3.5
-
 gamma-thiobutyrolactone 25°C, enzyme variant R192 Homo sapiens
3.1.1.25 5.3
-
 gamma-butyrolactone 25°C, enzyme variant R192 Homo sapiens
3.1.1.25 15
-
 gamma-butyrolactone 25°C, enzyme variant Q192 Homo sapiens
3.1.8.1 0.27
-
 paraoxon enzyme variant R192 Homo sapiens
3.1.8.1 0.5
-
 paraoxon enzyme variant Q192 Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.2 Ca2+ required for activity Homo sapiens
3.1.1.25 Ca2+ required for activity Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.2 Homo sapiens P27169 variant Q192 and R192
-
3.1.1.25 Homo sapiens P27169 variant Q192 and R192
-
3.1.8.1 Homo sapiens P27169 variant Q192 and R192
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.2
-
 Homo sapiens
3.1.1.25
-
 Homo sapiens
3.1.8.1
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.2 blood serum
-
 Homo sapiens
-
3.1.1.25 blood serum
-
 Homo sapiens
-
3.1.8.1 blood serum
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.2 phenyl acetate + H2O enzyme protein also shows activities of EC 3.1.8.1 and EC 3.1.1.25 Homo sapiens phenol + acetate
-
 ?
3.1.1.25 (1R,5S)-oxabicyclooctenone + H2O enzyme variant Q192: 0.82% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 0.75% of the activity with phenyl acetate (cf. EC 3.1.1.2) Homo sapiens ?
-
 ?
3.1.1.25 (1S,5R)-oxabicyclooctenone + H2O enzyme variant Q192: 1.67% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 2.92% of the activity with phenyl acetate (cf. EC 3.1.1.2) Homo sapiens ?
-
 ?
3.1.1.25 (R)-alpha-hydroxy-gamma-butyrolactone + H2O enzyme variant Q192: 0.88% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 3.95% of the activity with phenyl acetate (cf. EC 3.1.1.2) Homo sapiens (R)-2-hydroxybutyric acid
-
 ?
3.1.1.25 (R)-dihydro-5-(hydroxymethyl)-2(3H)-furanone + H2O enzyme variant Q192: 1.23% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 3.29% of the activity with phenyl acetate (cf. EC 3.1.1.2) Homo sapiens ?
-
 ?
3.1.1.25 (S)-alpha-hydroxy-gamma-butyrolactone + H2O enzyme variant Q192: 8.14% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 19.6% of the activity with phenyl acetate (cf. EC 3.1.1.2) Homo sapiens (S)-2-hydroxybutyric acid
-
 ?
3.1.1.25 (S)-beta-hydroxy-gamma-butyrolactone + H2O enzyme variant Q192: 0.6% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 0.76% of the activity with phenyl acetate (cf. EC 3.1.1.2) Homo sapiens (S)-3-hydroxybutyric acid
-
 ?
3.1.1.25 (S)-dihydro-5-(hydroxymethyl)-2(3H)-furanone + H2O enzyme variant Q192: 0.78% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 1.12% of the activity with phenyl acetate (cf. EC 3.1.1.2) Homo sapiens ?
-
 ?
3.1.1.25 2-coumaranone + H2O enzyme variant Q192: 18.3% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 13.5% of the activity with phenyl acetate (cf. EC 3.1.1.2) Homo sapiens (2-hydroxyphenyl)acetic acid
-
 ?
3.1.1.25 4-(1-propenyloxymethyl)-1,3-dioxolan-2-one + H2O enzyme variant Q192: 2.23% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 2.52% of the activity with phenyl acetate (cf. EC 3.1.1.2) Homo sapiens ?
-
 ?
3.1.1.25 alpha-angelicalactone + H2O enzyme variant Q192: 19.9% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 14.8% of the activity with phenyl acetate (cf. EC 3.1.1.2) Homo sapiens ?
-
 ?
3.1.1.25 alpha-bromo-gamma-butyrolactone + H2O enzyme variant Q192: 47.2% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 40.8% of the activity with phenyl acetate (cf. EC 3.1.1.2) Homo sapiens 2-bromobutyric acid
-
 ?
3.1.1.25 beta-hydroxybutyrolactone + H2O enzyme variant Q192: 3.83% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 7.53% of the activity with phenyl acetate (cf. EC 3.1.1.2) Homo sapiens ?
-
 ?
3.1.1.25 delta-decanolactone + H2O enzyme variant Q192: 9.65% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 12.8% of the activity with phenyl acetate (cf. EC 3.1.1.2) Homo sapiens 5-hydroxydecanoic acid
-
 ?
3.1.1.25 delta-valerolactone + H2O enzyme variant Q192: 75.4% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 71.0% of the activity with phenyl acetate (cf. EC 3.1.1.2) Homo sapiens 5-hydroxypentanoic acid
-
 ?
3.1.1.25 dihydrocoumarin + H2O enzyme variant Q192: 14.3% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 17% of the activity with phenyl acetate (cf. EC 3.1.1.2) Homo sapiens 3-(2-hydroxyphenyl)propanoic acid
-
 ?
3.1.1.25 epsilon-caprolactone + H2O enzyme variant Q192: 14.8% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 25.7% of the activity with phenyl acetate (cf. EC 3.1.1.2) Homo sapiens 6-hydroxyhexanoic acid
-
 ?
3.1.1.25 gamma-butyrolactone + H2O enzyme variant Q192: 2.46% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 9.05% of the activity with phenyl acetate (cf. EC 3.1.1.2) Homo sapiens 4-hydroxybutyric acid
-
 ?
3.1.1.25 gamma-decanolactone + H2O enzyme variant Q192: 12.4% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 19.0% of the activity with phenyl acetate (cf. EC 3.1.1.2) Homo sapiens 4-hydroxydecanoic acid
-
 ?
3.1.1.25 gamma-thiobutyrolactone + H2O
-
 Homo sapiens ?
-
 ?
3.1.1.25 gamma-valerolactone + H2O enzyme variant Q192: 7.28% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 6.97% of the activity with phenyl acetate (cf. EC 3.1.1.2) Homo sapiens 4-hydroxypentanoic acid
-
 ?
3.1.1.25 homogentisic acid lactone + H2O enzyme variant Q192: 44% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 49.7% of the activity with phenyl acetate (cf. EC 3.1.1.2) Homo sapiens homogentisic acid
-
 ?
3.1.1.25 lovastatin + H2O activity with enzyme variant Q192 and R192 Homo sapiens ?
-
 ?
3.1.1.25 mevastatin + H2O activity with enzyme variant Q192 and R192 Homo sapiens ?
-
 ?
3.1.1.25 additional information enzyme protein also shows activities of paraoxonase (EC 3.1.8.1) and arylesterase (EC 3.1.1.2) Homo sapiens ?
-
 ?
3.1.1.25 propylene carbonate + H2O enzyme variant Q192: 5.02% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 8.8% of the activity with phenyl acetate (cf. EC 3.1.1.2) Homo sapiens ?
-
 ?
3.1.1.25 simvastatin + H2O activity with enzyme variant Q192 and R192 Homo sapiens ?
-
 ?
3.1.1.25 spironolactone + H2O activity with enzyme variant Q192 and R192 Homo sapiens ?
-
 ?
3.1.1.25 undecano-gamma-lactone + H2O enzyme variant Q192: 11.8% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 12.7% of the activity with phenyl acetate (cf. EC 3.1.1.2) Homo sapiens 4-hydroxyundecanoic acid
-
 ?
3.1.8.1 paraoxon + H2O enzyme variant Q192: 1.3% of the activity with phenyl acetate (see EC 3.1.1.2), enzyme variant R192: 1% of the activity with phenyl acetate (see EC 3.1.1.2). Enzyme protein also shows activities of EC 3.1.1.25 and EC 3.1.1.2 Homo sapiens 4-nitrophenol + diethyl phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.8.1 PON1
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.25 25
-
 assay at Homo sapiens