BRENDA - Enzyme Database

Human serum paraoxonase (PON1) isozymes Q and R hydrolyze lactones and cyclic carbonate esters

Billecke, S.; Draganov, D.; Counsell, R.; Stetson, P.; Watson, C.; Hsu, C.; La Du, B.N.; Drug Metab. Dispos. 28, 1335-1342 (2000) View publication on PubMed

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.1.2
dilauroyl phosphatidylcholine
stimulates activity of enzyme variants Q192 and R192
Homo sapiens
3.1.1.25
dilauroyl phosphatidylcholine
stimulates activity of enzyme variants Q192 and R192
Homo sapiens
Engineering
EC Number
Protein Variants
Commentary
Organism
3.1.1.2
C284A
mutant with 44fold reduced paraoxonase activity
Homo sapiens
3.1.1.25
C284A
no lactonase activity with either homogentisic acid lactone or undecanoic-delta-lactone
Homo sapiens
3.1.8.1
C284A
mutant with 20fold reduced paraoxonase activity
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.2
Mg2+
activity is strongly reduced when Ca2+ is removed from the purified enzyme and replaced by Zn2+
Homo sapiens
3.1.1.2
Zn2+
activity is strongly reduced when Ca2+ is removed from the purified enzyme and replaced by Zn2+
Homo sapiens
3.1.1.25
Mg2+
activity is strongly reduced when Ca2+ is removed from the purified enzyme and replaced by Mg2+
Homo sapiens
3.1.1.25
Zn2+
activity is strongly reduced when Ca2+ is removed from the purified enzyme and replaced by Zn2+
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.1.2
0.27
-
phenyl acetate
enzyme variant R192
Homo sapiens
3.1.1.2
0.7
-
phenyl acetate
enzyme variant Q192
Homo sapiens
3.1.1.25
0.013
-
dihydrocoumarin
25°C, enzyme variant R192
Homo sapiens
3.1.1.25
0.013
-
2-coumaranone
25°C, enzyme variant R192
Homo sapiens
3.1.1.25
0.02
-
2-coumaranone
25°C, enzyme variant Q192
Homo sapiens
3.1.1.25
0.022
-
dihydrocoumarin
25°C, enzyme variant Q192
Homo sapiens
3.1.1.25
0.45
-
Homogentisic acid lactone
25°C, enzyme variant R192
Homo sapiens
3.1.1.25
0.52
-
Homogentisic acid lactone
25°C, enzyme variant Q192
Homo sapiens
3.1.1.25
2.8
-
gamma-thiobutyrolactone
25°C, enzyme variant Q192
Homo sapiens
3.1.1.25
3.5
-
gamma-thiobutyrolactone
25°C, enzyme variant R192
Homo sapiens
3.1.1.25
5.3
-
gamma-butyrolactone
25°C, enzyme variant R192
Homo sapiens
3.1.1.25
15
-
gamma-butyrolactone
25°C, enzyme variant Q192
Homo sapiens
3.1.8.1
0.27
-
paraoxon
enzyme variant R192
Homo sapiens
3.1.8.1
0.5
-
paraoxon
enzyme variant Q192
Homo sapiens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.1.2
Ca2+
required for activity
Homo sapiens
3.1.1.25
Ca2+
required for activity
Homo sapiens
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.1.1.2
Homo sapiens
P27169
variant Q192 and R192
-
3.1.1.25
Homo sapiens
P27169
variant Q192 and R192
-
3.1.8.1
Homo sapiens
P27169
variant Q192 and R192
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
3.1.1.2
-
Homo sapiens
3.1.1.25
-
Homo sapiens
3.1.8.1
-
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.2
blood serum
-
Homo sapiens
-
3.1.1.25
blood serum
-
Homo sapiens
-
3.1.8.1
blood serum
-
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.1.1.2
phenyl acetate + H2O
enzyme protein also shows activities of EC 3.1.8.1 and EC 3.1.1.25
650953
Homo sapiens
phenol + acetate
-
-
-
?
3.1.1.25
(1R,5S)-oxabicyclooctenone + H2O
enzyme variant Q192: 0.82% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 0.75% of the activity with phenyl acetate (cf. EC 3.1.1.2)
650953
Homo sapiens
?
-
-
-
?
3.1.1.25
(1S,5R)-oxabicyclooctenone + H2O
enzyme variant Q192: 1.67% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 2.92% of the activity with phenyl acetate (cf. EC 3.1.1.2)
650953
Homo sapiens
?
-
-
-
?
3.1.1.25
(R)-alpha-hydroxy-gamma-butyrolactone + H2O
enzyme variant Q192: 0.88% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 3.95% of the activity with phenyl acetate (cf. EC 3.1.1.2)
650953
Homo sapiens
(R)-2-hydroxybutyric acid
-
-
-
?
3.1.1.25
(R)-dihydro-5-(hydroxymethyl)-2(3H)-furanone + H2O
enzyme variant Q192: 1.23% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 3.29% of the activity with phenyl acetate (cf. EC 3.1.1.2)
650953
Homo sapiens
?
-
-
-
?
3.1.1.25
(S)-alpha-hydroxy-gamma-butyrolactone + H2O
enzyme variant Q192: 8.14% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 19.6% of the activity with phenyl acetate (cf. EC 3.1.1.2)
650953
Homo sapiens
(S)-2-hydroxybutyric acid
-
-
-
?
3.1.1.25
(S)-beta-hydroxy-gamma-butyrolactone + H2O
enzyme variant Q192: 0.6% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 0.76% of the activity with phenyl acetate (cf. EC 3.1.1.2)
650953
Homo sapiens
(S)-3-hydroxybutyric acid
-
-
-
?
3.1.1.25
(S)-dihydro-5-(hydroxymethyl)-2(3H)-furanone + H2O
enzyme variant Q192: 0.78% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 1.12% of the activity with phenyl acetate (cf. EC 3.1.1.2)
650953
Homo sapiens
?
-
-
-
?
3.1.1.25
2-coumaranone + H2O
enzyme variant Q192: 18.3% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 13.5% of the activity with phenyl acetate (cf. EC 3.1.1.2)
650953
Homo sapiens
(2-hydroxyphenyl)acetic acid
-
-
-
?
3.1.1.25
4-(1-propenyloxymethyl)-1,3-dioxolan-2-one + H2O
enzyme variant Q192: 2.23% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 2.52% of the activity with phenyl acetate (cf. EC 3.1.1.2)
650953
Homo sapiens
?
-
-
-
?
3.1.1.25
alpha-angelicalactone + H2O
enzyme variant Q192: 19.9% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 14.8% of the activity with phenyl acetate (cf. EC 3.1.1.2)
650953
Homo sapiens
?
-
-
-
?
3.1.1.25
alpha-bromo-gamma-butyrolactone + H2O
enzyme variant Q192: 47.2% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 40.8% of the activity with phenyl acetate (cf. EC 3.1.1.2)
650953
Homo sapiens
2-bromobutyric acid
-
-
-
?
3.1.1.25
beta-hydroxybutyrolactone + H2O
enzyme variant Q192: 3.83% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 7.53% of the activity with phenyl acetate (cf. EC 3.1.1.2)
650953
Homo sapiens
?
-
-
-
?
3.1.1.25
delta-decanolactone + H2O
enzyme variant Q192: 9.65% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 12.8% of the activity with phenyl acetate (cf. EC 3.1.1.2)
650953
Homo sapiens
5-hydroxydecanoic acid
-
-
-
?
3.1.1.25
delta-valerolactone + H2O
enzyme variant Q192: 75.4% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 71.0% of the activity with phenyl acetate (cf. EC 3.1.1.2)
650953
Homo sapiens
5-hydroxypentanoic acid
-
-
-
?
3.1.1.25
dihydrocoumarin + H2O
enzyme variant Q192: 14.3% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 17% of the activity with phenyl acetate (cf. EC 3.1.1.2)
650953
Homo sapiens
3-(2-hydroxyphenyl)propanoic acid
-
-
-
?
3.1.1.25
epsilon-caprolactone + H2O
enzyme variant Q192: 14.8% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 25.7% of the activity with phenyl acetate (cf. EC 3.1.1.2)
650953
Homo sapiens
5-hydroxyhexanoic acid
-
-
-
?
3.1.1.25
gamma-butyrolactone + H2O
enzyme variant Q192: 2.46% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 9.05% of the activity with phenyl acetate (cf. EC 3.1.1.2)
650953
Homo sapiens
butyric acid
-
-
-
?
3.1.1.25
gamma-decanolactone + H2O
enzyme variant Q192: 12.4% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 19.0% of the activity with phenyl acetate (cf. EC 3.1.1.2)
650953
Homo sapiens
4-hydroxydecanoic acid
-
-
-
?
3.1.1.25
gamma-thiobutyrolactone + H2O
-
650953
Homo sapiens
?
-
-
-
?
3.1.1.25
gamma-valerolactone + H2O
enzyme variant Q192: 7.28% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 6.97% of the activity with phenyl acetate (cf. EC 3.1.1.2)
650953
Homo sapiens
4-hydroxypentanoic acid
-
-
-
?
3.1.1.25
homogentisic acid lactone + H2O
enzyme variant Q192: 44% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 49.7% of the activity with phenyl acetate (cf. EC 3.1.1.2)
650953
Homo sapiens
homogentisic acid
-
-
-
?
3.1.1.25
lovastatin + H2O
activity with enzyme variant Q192 and R192
650953
Homo sapiens
?
-
-
-
?
3.1.1.25
mevastatin + H2O
activity with enzyme variant Q192 and R192
650953
Homo sapiens
?
-
-
-
?
3.1.1.25
additional information
enzyme protein also shows activities of paraoxonase (EC 3.1.8.1) and arylesterase (EC 3.1.1.2)
650953
Homo sapiens
?
-
-
-
?
3.1.1.25
propylene carbonate + H2O
enzyme variant Q192: 5.02% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 8.8% of the activity with phenyl acetate (cf. EC 3.1.1.2)
650953
Homo sapiens
?
-
-
-
?
3.1.1.25
simvastatin + H2O
activity with enzyme variant Q192 and R192
650953
Homo sapiens
?
-
-
-
?
3.1.1.25
spironolactone + H2O
activity with enzyme variant Q192 and R192
650953
Homo sapiens
?
-
-
-
?
3.1.1.25
undecano-gamma-lactone + H2O
enzyme variant Q192: 11.8% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 12.7% of the activity with phenyl acetate (cf. EC 3.1.1.2)
650953
Homo sapiens
4-hydroxyundecanoic acid
-
-
-
?
3.1.8.1
paraoxon + H2O
enzyme variant Q192: 1.3% of the activity with phenyl acetate (see EC 3.1.1.2), enzyme variant R192: 1% of the activity with phenyl acetate (see EC 3.1.1.2). Enzyme protein also shows activities of EC 3.1.1.25 and EC 3.1.1.2
650953
Homo sapiens
4-nitrophenol + diethyl phosphate
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
3.1.8.1
PON1
-
Homo sapiens
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.1.25
25
-
assay at
Homo sapiens
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.1.2
dilauroyl phosphatidylcholine
stimulates activity of enzyme variants Q192 and R192
Homo sapiens
3.1.1.25
dilauroyl phosphatidylcholine
stimulates activity of enzyme variants Q192 and R192
Homo sapiens
Engineering (protein specific)
EC Number
Protein Variants
Commentary
Organism
3.1.1.2
C284A
mutant with 44fold reduced paraoxonase activity
Homo sapiens
3.1.1.25
C284A
no lactonase activity with either homogentisic acid lactone or undecanoic-delta-lactone
Homo sapiens
3.1.8.1
C284A
mutant with 20fold reduced paraoxonase activity
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.2
Mg2+
activity is strongly reduced when Ca2+ is removed from the purified enzyme and replaced by Zn2+
Homo sapiens
3.1.1.2
Zn2+
activity is strongly reduced when Ca2+ is removed from the purified enzyme and replaced by Zn2+
Homo sapiens
3.1.1.25
Mg2+
activity is strongly reduced when Ca2+ is removed from the purified enzyme and replaced by Mg2+
Homo sapiens
3.1.1.25
Zn2+
activity is strongly reduced when Ca2+ is removed from the purified enzyme and replaced by Zn2+
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.1.2
0.27
-
phenyl acetate
enzyme variant R192
Homo sapiens
3.1.1.2
0.7
-
phenyl acetate
enzyme variant Q192
Homo sapiens
3.1.1.25
0.013
-
dihydrocoumarin
25°C, enzyme variant R192
Homo sapiens
3.1.1.25
0.013
-
2-coumaranone
25°C, enzyme variant R192
Homo sapiens
3.1.1.25
0.02
-
2-coumaranone
25°C, enzyme variant Q192
Homo sapiens
3.1.1.25
0.022
-
dihydrocoumarin
25°C, enzyme variant Q192
Homo sapiens
3.1.1.25
0.45
-
Homogentisic acid lactone
25°C, enzyme variant R192
Homo sapiens
3.1.1.25
0.52
-
Homogentisic acid lactone
25°C, enzyme variant Q192
Homo sapiens
3.1.1.25
2.8
-
gamma-thiobutyrolactone
25°C, enzyme variant Q192
Homo sapiens
3.1.1.25
3.5
-
gamma-thiobutyrolactone
25°C, enzyme variant R192
Homo sapiens
3.1.1.25
5.3
-
gamma-butyrolactone
25°C, enzyme variant R192
Homo sapiens
3.1.1.25
15
-
gamma-butyrolactone
25°C, enzyme variant Q192
Homo sapiens
3.1.8.1
0.27
-
paraoxon
enzyme variant R192
Homo sapiens
3.1.8.1
0.5
-
paraoxon
enzyme variant Q192
Homo sapiens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.1.2
Ca2+
required for activity
Homo sapiens
3.1.1.25
Ca2+
required for activity
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.1.2
-
Homo sapiens
3.1.1.25
-
Homo sapiens
3.1.8.1
-
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.2
blood serum
-
Homo sapiens
-
3.1.1.25
blood serum
-
Homo sapiens
-
3.1.8.1
blood serum
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.1.1.2
phenyl acetate + H2O
enzyme protein also shows activities of EC 3.1.8.1 and EC 3.1.1.25
650953
Homo sapiens
phenol + acetate
-
-
-
?
3.1.1.25
(1R,5S)-oxabicyclooctenone + H2O
enzyme variant Q192: 0.82% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 0.75% of the activity with phenyl acetate (cf. EC 3.1.1.2)
650953
Homo sapiens
?
-
-
-
?
3.1.1.25
(1S,5R)-oxabicyclooctenone + H2O
enzyme variant Q192: 1.67% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 2.92% of the activity with phenyl acetate (cf. EC 3.1.1.2)
650953
Homo sapiens
?
-
-
-
?
3.1.1.25
(R)-alpha-hydroxy-gamma-butyrolactone + H2O
enzyme variant Q192: 0.88% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 3.95% of the activity with phenyl acetate (cf. EC 3.1.1.2)
650953
Homo sapiens
(R)-2-hydroxybutyric acid
-
-
-
?
3.1.1.25
(R)-dihydro-5-(hydroxymethyl)-2(3H)-furanone + H2O
enzyme variant Q192: 1.23% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 3.29% of the activity with phenyl acetate (cf. EC 3.1.1.2)
650953
Homo sapiens
?
-
-
-
?
3.1.1.25
(S)-alpha-hydroxy-gamma-butyrolactone + H2O
enzyme variant Q192: 8.14% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 19.6% of the activity with phenyl acetate (cf. EC 3.1.1.2)
650953
Homo sapiens
(S)-2-hydroxybutyric acid
-
-
-
?
3.1.1.25
(S)-beta-hydroxy-gamma-butyrolactone + H2O
enzyme variant Q192: 0.6% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 0.76% of the activity with phenyl acetate (cf. EC 3.1.1.2)
650953
Homo sapiens
(S)-3-hydroxybutyric acid
-
-
-
?
3.1.1.25
(S)-dihydro-5-(hydroxymethyl)-2(3H)-furanone + H2O
enzyme variant Q192: 0.78% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 1.12% of the activity with phenyl acetate (cf. EC 3.1.1.2)
650953
Homo sapiens
?
-
-
-
?
3.1.1.25
2-coumaranone + H2O
enzyme variant Q192: 18.3% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 13.5% of the activity with phenyl acetate (cf. EC 3.1.1.2)
650953
Homo sapiens
(2-hydroxyphenyl)acetic acid
-
-
-
?
3.1.1.25
4-(1-propenyloxymethyl)-1,3-dioxolan-2-one + H2O
enzyme variant Q192: 2.23% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 2.52% of the activity with phenyl acetate (cf. EC 3.1.1.2)
650953
Homo sapiens
?
-
-
-
?
3.1.1.25
alpha-angelicalactone + H2O
enzyme variant Q192: 19.9% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 14.8% of the activity with phenyl acetate (cf. EC 3.1.1.2)
650953
Homo sapiens
?
-
-
-
?
3.1.1.25
alpha-bromo-gamma-butyrolactone + H2O
enzyme variant Q192: 47.2% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 40.8% of the activity with phenyl acetate (cf. EC 3.1.1.2)
650953
Homo sapiens
2-bromobutyric acid
-
-
-
?
3.1.1.25
beta-hydroxybutyrolactone + H2O
enzyme variant Q192: 3.83% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 7.53% of the activity with phenyl acetate (cf. EC 3.1.1.2)
650953
Homo sapiens
?
-
-
-
?
3.1.1.25
delta-decanolactone + H2O
enzyme variant Q192: 9.65% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 12.8% of the activity with phenyl acetate (cf. EC 3.1.1.2)
650953
Homo sapiens
5-hydroxydecanoic acid
-
-
-
?
3.1.1.25
delta-valerolactone + H2O
enzyme variant Q192: 75.4% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 71.0% of the activity with phenyl acetate (cf. EC 3.1.1.2)
650953
Homo sapiens
5-hydroxypentanoic acid
-
-
-
?
3.1.1.25
dihydrocoumarin + H2O
enzyme variant Q192: 14.3% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 17% of the activity with phenyl acetate (cf. EC 3.1.1.2)
650953
Homo sapiens
3-(2-hydroxyphenyl)propanoic acid
-
-
-
?
3.1.1.25
epsilon-caprolactone + H2O
enzyme variant Q192: 14.8% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 25.7% of the activity with phenyl acetate (cf. EC 3.1.1.2)
650953
Homo sapiens
5-hydroxyhexanoic acid
-
-
-
?
3.1.1.25
gamma-butyrolactone + H2O
enzyme variant Q192: 2.46% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 9.05% of the activity with phenyl acetate (cf. EC 3.1.1.2)
650953
Homo sapiens
butyric acid
-
-
-
?
3.1.1.25
gamma-decanolactone + H2O
enzyme variant Q192: 12.4% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 19.0% of the activity with phenyl acetate (cf. EC 3.1.1.2)
650953
Homo sapiens
4-hydroxydecanoic acid
-
-
-
?
3.1.1.25
gamma-thiobutyrolactone + H2O
-
650953
Homo sapiens
?
-
-
-
?
3.1.1.25
gamma-valerolactone + H2O
enzyme variant Q192: 7.28% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 6.97% of the activity with phenyl acetate (cf. EC 3.1.1.2)
650953
Homo sapiens
4-hydroxypentanoic acid
-
-
-
?
3.1.1.25
homogentisic acid lactone + H2O
enzyme variant Q192: 44% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 49.7% of the activity with phenyl acetate (cf. EC 3.1.1.2)
650953
Homo sapiens
homogentisic acid
-
-
-
?
3.1.1.25
lovastatin + H2O
activity with enzyme variant Q192 and R192
650953
Homo sapiens
?
-
-
-
?
3.1.1.25
mevastatin + H2O
activity with enzyme variant Q192 and R192
650953
Homo sapiens
?
-
-
-
?
3.1.1.25
additional information
enzyme protein also shows activities of paraoxonase (EC 3.1.8.1) and arylesterase (EC 3.1.1.2)
650953
Homo sapiens
?
-
-
-
?
3.1.1.25
propylene carbonate + H2O
enzyme variant Q192: 5.02% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 8.8% of the activity with phenyl acetate (cf. EC 3.1.1.2)
650953
Homo sapiens
?
-
-
-
?
3.1.1.25
simvastatin + H2O
activity with enzyme variant Q192 and R192
650953
Homo sapiens
?
-
-
-
?
3.1.1.25
spironolactone + H2O
activity with enzyme variant Q192 and R192
650953
Homo sapiens
?
-
-
-
?
3.1.1.25
undecano-gamma-lactone + H2O
enzyme variant Q192: 11.8% of the activity with phenyl acetate (cf. EC 3.1.1.2), enzyme variant R192: 12.7% of the activity with phenyl acetate (cf. EC 3.1.1.2)
650953
Homo sapiens
4-hydroxyundecanoic acid
-
-
-
?
3.1.8.1
paraoxon + H2O
enzyme variant Q192: 1.3% of the activity with phenyl acetate (see EC 3.1.1.2), enzyme variant R192: 1% of the activity with phenyl acetate (see EC 3.1.1.2). Enzyme protein also shows activities of EC 3.1.1.25 and EC 3.1.1.2
650953
Homo sapiens
4-nitrophenol + diethyl phosphate
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.1.25
25
-
assay at
Homo sapiens