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Literature summary extracted from

  • Kuo, L.Y.; Hwang, G.Y.; Lai, Y.J.; Yang, S.L.; Lin, L.L.
    Overexpression, purification, and characterization of the recombinant leucine aminopeptidase II of Bacillus stearothermophilus (2003), Curr. Microbiol., 47, 40-45.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.22 overexpression in Escherichia coli as His-tagged enzyme Geobacillus stearothermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.22 Ca2+ slight inhibition Geobacillus stearothermophilus
3.4.11.22 Cu2+ complete inhibition at 1 mM, recombinant enzyme Geobacillus stearothermophilus
3.4.11.22 DTT complete inhibition at 10 mM Geobacillus stearothermophilus
3.4.11.22 EDTA complete inhibition at 10 mM, Co2+ reactivates, only slight reactivation by Ca2+, Mn2+ and Mg2+, recombinant enzyme Geobacillus stearothermophilus
3.4.11.22 Hg2+ 99% inhibition at 1 mM, recombinant enzyme Geobacillus stearothermophilus
3.4.11.22 Mn2+ 73% inhibition at 1 mM Geobacillus stearothermophilus
3.4.11.22 additional information not affected by Mg2+ Geobacillus stearothermophilus
3.4.11.22 Ni2+ 89% inhibition at 1 mM Geobacillus stearothermophilus
3.4.11.22 PMSF complete inhibition at 10 mM Geobacillus stearothermophilus
3.4.11.22 Zn2+ 94% inhibition at 1 mM Geobacillus stearothermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.22 Co2+ activates 3.8fold at 1 mM, dependent on, recombinant enzyme Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.22 44500
-
 x * 44500, recombinant enzyme, SDS-PAGE Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.22 Geobacillus stearothermophilus
-
-
-
3.4.11.22 Geobacillus stearothermophilus NIB 8924
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.22 recombinant His-tagged enzyme from Escherichia coli, 9.2fold to homogeneity Geobacillus stearothermophilus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.11.22 Release of an N-terminal amino acid, preferably a neutral or hydrophobic one, from a polypeptide. Aminoacyl-arylamides are poor substrates cobalt-dependent exopeptidase Geobacillus stearothermophilus
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.22 425
-
 purified recombinant enzyme Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.22 L-Arg-4-nitroanilide + H2O
-
 Geobacillus stearothermophilus L-Arg + 4-nitroaniline
-
 ?
3.4.11.22 L-Arg-4-nitroanilide + H2O
-
 Geobacillus stearothermophilus NIB 8924 L-Arg + 4-nitroaniline
-
 ?
3.4.11.22 L-Leu-4-nitroanilide + H2O preferred substrate, recombinant enzyme Geobacillus stearothermophilus L-Leu + 4-nitroaniline
-
 ?
3.4.11.22 L-Leu-4-nitroanilide + H2O preferred substrate, recombinant enzyme Geobacillus stearothermophilus NIB 8924 L-Leu + 4-nitroaniline
-
 ?
3.4.11.22 L-Lys-4-nitroanilide + H2O
-
 Geobacillus stearothermophilus L-Lys + 4-nitroaniline
-
 ?
3.4.11.22 L-Lys-4-nitroanilide + H2O
-
 Geobacillus stearothermophilus NIB 8924 L-Lys + 4-nitroaniline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.22 ? x * 44500, recombinant enzyme, SDS-PAGE Geobacillus stearothermophilus

Synonyms

EC Number Synonyms Comment Organism
3.4.11.22 LAP II
-
 Geobacillus stearothermophilus
3.4.11.22 leucine aminopeptidase II
-
 Geobacillus stearothermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.22 60
-
 recombinant enzyme Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.22 8
-
 recombinant enzyme Geobacillus stearothermophilus