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Literature summary extracted from
- Isolation and characteristics of carboxypeptidase B from the pyloric ceca of the starfish Asterias amurensis (2002), Comp. Biochem. Physiol. B, 133, 183-189.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.17.2 | EDTA | 1 mM, 6 h, 90% loss of activity | Asterias amurensis |  |
| 3.4.17.2 | Hg2+ | 1 mM, 97% inhibition | Asterias amurensis | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.17.2 | 0.71 | - |
benzoyl-Gly-Arg | - |
Asterias amurensis | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.17.2 | Co2+ | 1 mM, activation to 181% of control | Asterias amurensis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.17.2 | 34000 | - |
x * 34000, SDS-PAGE | Asterias amurensis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.17.2 | Asterias amurensis | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.17.2 | - |
Asterias amurensis |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.17.2 | pyloric cecum | - |
Asterias amurensis | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.4.17.2 | 460 | - |
- |
Asterias amurensis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.17.2 | benzoyl-Gly-Arg + H2O | - |
Asterias amurensis | benzoyl-Gly + Arg | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.17.2 | ? | x * 34000, SDS-PAGE | Asterias amurensis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.17.2 | 55 | - |
- |
Asterias amurensis |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.17.2 | 30 | 70 | 30°C: about 40% of maximal activity, 70°C: about 55% of maximal activity | Asterias amurensis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.17.2 | 30 | 45 | 15 min, stable | Asterias amurensis |
| 3.4.17.2 | 50 | - |
15 min, about 10% loss of activity | Asterias amurensis |
| 3.4.17.2 | 60 | - |
15 min, about 70% loss of activity | Asterias amurensis |
| 3.4.17.2 | 65 | - |
15 min, about 10% loss of activity | Asterias amurensis |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.17.2 | 427 | - |
benzoyl-Gly-Arg | - |
Asterias amurensis | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.17.2 | 7.5 | - |
- |
Asterias amurensis |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.17.2 | 5.5 | 9 | pH 5.5: about 60% of maximal activity, pH 9.0: about 30% of maximal activity | Asterias amurensis |
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