BRENDA - Enzyme Database

Isolation and characteristics of carboxypeptidase B from the pyloric ceca of the starfish Asterias amurensis

Kishimura, H.; Hayashi, K.; Comp. Biochem. Physiol. B 133, 183-189 (2002)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.4.17.2
EDTA
1 mM, 6 h, 90% loss of activity
Asterias amurensis
3.4.17.2
Hg2+
1 mM, 97% inhibition
Asterias amurensis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.4.17.2
0.71
-
benzoyl-Gly-Arg
-
Asterias amurensis
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.4.17.2
Co2+
1 mM, activation to 181% of control
Asterias amurensis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.4.17.2
34000
-
x * 34000, SDS-PAGE
Asterias amurensis
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.4.17.2
Asterias amurensis
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
3.4.17.2
-
Asterias amurensis
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.4.17.2
pyloric cecum
-
Asterias amurensis
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.4.17.2
460
-
-
Asterias amurensis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.4.17.2
benzoyl-Gly-Arg + H2O
-
650889
Asterias amurensis
benzoyl-Gly + Arg
-
650889
Asterias amurensis
?
Subunits
EC Number
Subunits
Commentary
Organism
3.4.17.2
?
x * 34000, SDS-PAGE
Asterias amurensis
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.4.17.2
55
-
-
Asterias amurensis
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
3.4.17.2
30
70
30°C: about 40% of maximal activity, 70°C: about 55% of maximal activity
Asterias amurensis
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.4.17.2
30
45
15 min, stable
Asterias amurensis
3.4.17.2
50
-
15 min, about 10% loss of activity
Asterias amurensis
3.4.17.2
60
-
15 min, about 70% loss of activity
Asterias amurensis
3.4.17.2
65
-
15 min, about 10% loss of activity
Asterias amurensis
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.4.17.2
427
-
benzoyl-Gly-Arg
-
Asterias amurensis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.4.17.2
7.5
-
-
Asterias amurensis
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.4.17.2
5.5
9
pH 5.5: about 60% of maximal activity, pH 9.0: about 30% of maximal activity
Asterias amurensis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.4.17.2
EDTA
1 mM, 6 h, 90% loss of activity
Asterias amurensis
3.4.17.2
Hg2+
1 mM, 97% inhibition
Asterias amurensis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.4.17.2
0.71
-
benzoyl-Gly-Arg
-
Asterias amurensis
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.4.17.2
Co2+
1 mM, activation to 181% of control
Asterias amurensis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.4.17.2
34000
-
x * 34000, SDS-PAGE
Asterias amurensis
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.4.17.2
-
Asterias amurensis
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.4.17.2
pyloric cecum
-
Asterias amurensis
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.4.17.2
460
-
-
Asterias amurensis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.4.17.2
benzoyl-Gly-Arg + H2O
-
650889
Asterias amurensis
benzoyl-Gly + Arg
-
650889
Asterias amurensis
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.4.17.2
?
x * 34000, SDS-PAGE
Asterias amurensis
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.4.17.2
55
-
-
Asterias amurensis
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
3.4.17.2
30
70
30°C: about 40% of maximal activity, 70°C: about 55% of maximal activity
Asterias amurensis
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.4.17.2
30
45
15 min, stable
Asterias amurensis
3.4.17.2
50
-
15 min, about 10% loss of activity
Asterias amurensis
3.4.17.2
60
-
15 min, about 70% loss of activity
Asterias amurensis
3.4.17.2
65
-
15 min, about 10% loss of activity
Asterias amurensis
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.4.17.2
427
-
benzoyl-Gly-Arg
-
Asterias amurensis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.4.17.2
7.5
-
-
Asterias amurensis
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.4.17.2
5.5
9
pH 5.5: about 60% of maximal activity, pH 9.0: about 30% of maximal activity
Asterias amurensis