EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.13.83 | gene cobG heterologously expressed in Escherichia coli | Pseudomonas denitrificans (nom. rej.) |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.83 | Fe2+ | iron-sulfur protein | Pseudomonas denitrificans (nom. rej.) |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.13.83 | 46000 | - |
SDS-PAGE | Pseudomonas denitrificans (nom. rej.) |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.83 | precorrin-3A + NADH + H+ + O2 | Pseudomonas denitrificans (nom. rej.) | biosynthesis of vitamin B12 | precorrin-3B + NAD+ + H2O | aerobic incubation with CobG alone, if CobJ and S-adenosyl-L-methionine are included the product is precorrin-4 | ir | |
1.14.13.83 | precorrin-3A + NADH + H+ + O2 | Pseudomonas denitrificans (nom. rej.) G3575 | biosynthesis of vitamin B12 | precorrin-3B + NAD+ + H2O | aerobic incubation with CobG alone, if CobJ and S-adenosyl-L-methionine are included the product is precorrin-4 | ir |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.83 | Pseudomonas denitrificans (nom. rej.) | - |
- |
- |
1.14.13.83 | Pseudomonas denitrificans (nom. rej.) G3575 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.13.83 | - |
Pseudomonas denitrificans (nom. rej.) |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.83 | precorrin-3A + NADH + H+ + O2 | - |
Pseudomonas denitrificans (nom. rej.) | precorrin-3B + NAD+ + H2O | aerobic incubation with CobG alone, if CobJ and S-adenosyl-L-methionine are included the product is precorrin-4 | ir | |
1.14.13.83 | precorrin-3A + NADH + H+ + O2 | biosynthesis of vitamin B12 | Pseudomonas denitrificans (nom. rej.) | precorrin-3B + NAD+ + H2O | aerobic incubation with CobG alone, if CobJ and S-adenosyl-L-methionine are included the product is precorrin-4 | ir | |
1.14.13.83 | precorrin-3A + NADH + H+ + O2 | - |
Pseudomonas denitrificans (nom. rej.) G3575 | precorrin-3B + NAD+ + H2O | aerobic incubation with CobG alone, if CobJ and S-adenosyl-L-methionine are included the product is precorrin-4 | ir | |
1.14.13.83 | precorrin-3A + NADH + H+ + O2 | biosynthesis of vitamin B12 | Pseudomonas denitrificans (nom. rej.) G3575 | precorrin-3B + NAD+ + H2O | aerobic incubation with CobG alone, if CobJ and S-adenosyl-L-methionine are included the product is precorrin-4 | ir |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.13.83 | CobG | - |
Pseudomonas denitrificans (nom. rej.) |