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Literature summary extracted from

  • Laurent, F.; Kotoujansky, A.; Bertheau, Y.
    Overproduction in Escherichia coli of the pectin methylesterase A from Erwinia chrysanthemi 3937: one-step purification, biochemical characterization, and production of polyclonal antibodies (2000), Can. J. Microbiol., 46, 474-480.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.11 expressed in Escherichia coli Dickeya chrysanthemi

General Stability

EC Number General Stability Organism
3.1.1.11 significant loss of activity after freezing and thawing Dickeya chrysanthemi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.11 additional information
-
 citrus pectin pH 6 and pH 7.6 Dickeya chrysanthemi

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.11 Dickeya chrysanthemi
-
-
-
3.1.1.11 Dickeya chrysanthemi 3937
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.11 homogeneity, one-step purification Dickeya chrysanthemi

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.11 additional information
-
-
 Dickeya chrysanthemi

Storage Stability

EC Number Storage Stability Organism
3.1.1.11 4°C, purified enzyme, stable for 1 week Dickeya chrysanthemi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.11 citrus pectin + H2O highest activity with pectin with an esterification degree of 50% Dickeya chrysanthemi methanol + citrus pectate
-
 ?
3.1.1.11 citrus pectin + H2O highest activity with pectin with an esterification degree of 50% Dickeya chrysanthemi 3937 methanol + citrus pectate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.11 50
-
-
 Dickeya chrysanthemi

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.11 50
-
 no loss of activity after 20 min Dickeya chrysanthemi
3.1.1.11 55
-
 50% loss of activity after 5 min Dickeya chrysanthemi
3.1.1.11 60
-
 complete loss of activity after 2 min Dickeya chrysanthemi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.11 8 9
-
 Dickeya chrysanthemi

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.11 5 6 about 55% activity at pH 5-6 Dickeya chrysanthemi