Literature summary extracted from

Rots, M.G.; Pieters, R.; Peters, G.J.; Noordhuis, P.; van Zantwijk, C.H.; Kaspers, G.J.; Hahlen, K.; Creutzig, U.; Veerman, A.J.; Jansen, G.
Role of folylpolyglutamate synthetase and folylpolyglutamate hydrolase in methotrexate accumulation and polyglutamylation in childhood leukemia (1999), Blood, 93, 1677-1683.

Organism

EC Number	Organism	UniProt	Comment	Textmining
6.3.2.17	Homo sapiens	-	human, children	-

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
6.3.2.17	bone marrow	-	Homo sapiens	-
6.3.2.17	peripheral blood	-	Homo sapiens	-

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
6.3.2.17	0.00000001	-	patients with acute myeloid leukemia, AML	Homo sapiens
6.3.2.17	0.000000099	-	patients with T-linage acute lymphoblastic leukemia, T-ALL	Homo sapiens
6.3.2.17	0.000000244	-	patients with c/preB-acute lymphoblastic leukemia, c/preB-ALL	Homo sapiens

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
6.3.2.17	ATP + methotrexate-Glu + L-glutamate	T-ALL and AML patients show inefficient polyglutamylation due to lower enzyme activity	Homo sapiens	ADP + methotrexate-Glu2 + phosphate	-	?

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Release 2023.1 (January 2023)