EC Number | Cloned (Comment) | Organism |
---|---|---|
4.1.2.47 | expressed in Escherichia coli BL21 (DE3) | Manihot esculenta |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.1.2.47 | G113S | enhanced thermal stability compared to wild-type enzyme, mutant enzyme retains slight higher activity than the wild-type enzyme in an acidic environment, so the mutant enzyme maybe more effective for synthesis of (S)-cyanohydrin than the wild-type enzyme | Manihot esculenta |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.2.47 | 1.1 | - |
mandelonitrile | pH 5.4, 25°C, mutant enzyme G113S | Manihot esculenta | |
4.1.2.47 | 1.4 | - |
mandelonitrile | pH 5.4, 25°C, wild-type enzyme | Manihot esculenta |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
4.1.2.47 | 29000 | - |
4 * 29000, SDS-PAGE | Manihot esculenta |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.2.47 | mandelonitrile | Manihot esculenta | - |
HCN + benzaldehyde | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.2.47 | Manihot esculenta | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.2.47 | recombinant enzyme | Manihot esculenta |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.1.2.47 | 35.6 | - |
wild-type enzyme | Manihot esculenta |
4.1.2.47 | 57.2 | - |
mutant enzyme G113S | Manihot esculenta |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.2.47 | mandelonitrile | - |
Manihot esculenta | HCN + benzaldehyde | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.1.2.47 | tetramer | 4 * 29000, SDS-PAGE | Manihot esculenta |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.2.47 | (S)-HNL | - |
Manihot esculenta |
4.1.2.47 | HNL | - |
Manihot esculenta |
4.1.2.47 | HNL1 | - |
Manihot esculenta |
4.1.2.47 | Hydroxynitrile lyase | - |
Manihot esculenta |
4.1.2.47 | MeHNL | - |
Manihot esculenta |
4.1.2.47 | mut-HNL1 | - |
Manihot esculenta |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.2.47 | 25 | - |
- |
Manihot esculenta |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.2.47 | 25 | 70 | - |
Manihot esculenta |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.2.47 | 45 | - |
mutant enzyme and wild-type enzyme G113S are stable for 20 min at pH 5.4 | Manihot esculenta |
4.1.2.47 | 60 | - |
20 min at pH 5.4, mutant enzyme G113S loses about 10% of its initial activity, wild-type enzyme loses about 20% of its initial activity. More than 50% loss of activity of wild-type enzyme after 50 min, 10% loss of activity of mutant enzyme G113 after 50 min, 75% loss of activity of wild-type enzyme after 120 min, 25% loss of activity of mutant enzyme G113 after 120 min | Manihot esculenta |
4.1.2.47 | 65 | - |
20 min at pH 5.4, mutant enzyme G113S loses about 15% of its initial activity, wild-type enzyme loses about 50% of its initial activity | Manihot esculenta |
4.1.2.47 | 70 | - |
20 min at pH 5.4, about 80% loss of activity, mutant enzyme G113S and wild-type enzyme | Manihot esculenta |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.1.2.47 | 5.5 | - |
mutant enzyme G113S | Manihot esculenta |
4.1.2.47 | 5.6 | - |
wild-type enzyme | Manihot esculenta |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
4.1.2.47 | 4.5 | 6.5 | pH 4.5: about 65% of maximal activity of mutant enzyme G113S, about 60% of maximal activity of wild-type enzyme, pH 6.5: about 50% of maximal activity of mutant enzyme G113S, about 55% of maximal activity of wild-type enzyme | Manihot esculenta |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.1.2.47 | additional information | FAD-independent | Manihot esculenta |