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Literature summary extracted from

  • Patel, O.; Castelli, L.; Fernley, R.; Coloe, P.; Macreadie, I.
    Production of a functional dihydrofolate synthase with a cleavable poly-His tag in Saccharomyces cerevisiae (2002), Biotechnol. Lett., 24, 657-662.
    View publication on PubMed

Application

EC Number Application Comment Organism
6.3.2.12 biotechnology production of active dihidrofolate synthase in milligram scale Saccharomyces cerevisiae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.12 expression of a histidine-tagged recombinant enzyme in a Saccharomyces cerevisiae lacking a DHFS functional gene Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.12 Saccharomyces cerevisiae
-
 CUY13 strain
-
6.3.2.12 Saccharomyces cerevisiae CUY13
-
 CUY13 strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.12 purification of a histidine-tagged recombinant enzyme using metal-affinity chromatography Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.12 ATP + 7,8-dihydropteroate + L-Glu
-
 Saccharomyces cerevisiae ADP + phosphate + 7,8-dihydrofolate
-
 ?
6.3.2.12 ATP + 7,8-dihydropteroate + L-Glu
-
 Saccharomyces cerevisiae CUY13 ADP + phosphate + 7,8-dihydrofolate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.2.12 DHFS
-
 Saccharomyces cerevisiae