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Literature summary extracted from

  • Babe, L.M.; Yoast, S.; Dreyer, M.; Schmidt, B.F.
    Heterologous expression of human granzyme K in Bacillus subtilis and characterization of its hydrolytic activity in vitro (1998), Biotechnol. Appl. Biochem., 27, 117-124.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.B4 expressed in Bacillus subtilis Homo sapiens

General Stability

EC Number General Stability Organism
3.4.21.B4 enzyme quickly loses catalytic activity when diluted and stored in solution, Tween-20 or bovine serum albumin prevent loss of activity Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.B4 3,4-dichloroisocoumarin complete inactivation at 0.1 mM Homo sapiens
3.4.21.B4 benzamidine 50% inhibition at 27 mM Homo sapiens
3.4.21.B4 leupeptin 50% inhibition at 0.2 mM Homo sapiens
3.4.21.B4 phenylmethylsulfonyl fluoride complete inactivation at 2 mM Homo sapiens
3.4.21.B4 Soybean trypsin inhibitor 50% inhibition at 0.025 mM Homo sapiens
3.4.21.B4 Zn2+ significant reduction of enzyme activity Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.B4 28000
-
 SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.B4 Protein + H2O Homo sapiens
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.B4 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.B4
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.B4 T-lymphocyte cytotoxic Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.B4 Cys-Gly-Tyr-Gly-Pro-Lys-Lys-Lys-Arg-Lys-Val-Gly-Gly + H2O
-
 Homo sapiens ?
-
 ?
3.4.21.B4 additional information for single-residue thioester substrates the enzyme shows a 2fold preference for a Lys versus Arg residue at P1. With oligopeptide substrates the enzyme displays peptidolytic activity C-terminal to both Lys and Arg residues with comparable rates of hydrolysis Homo sapiens ?
-
 ?
3.4.21.B4 Protein + H2O
-
 Homo sapiens ?
-
 ?
3.4.21.B4 Z-Lys-thiobenzyl ester + H2O
-
 Homo sapiens ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.B4 7.5
-
-
 Homo sapiens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.21.B4 Homo sapiens calculated from nucleotide sequence
-
 10.2