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Literature summary extracted from

  • Van den Burg, B.; Eijsink, V.G.H.; Vriend, G.; Veltman, O.R.; Venema, G.
    Rendering one autolysis site in Bacillus subtilis neutral protease resistant to cleavage reveals a new fission (1998), Biotechnol. Appl. Biochem., 27, 125-132.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.28 protease-deficient strain Bacillus subtilis DB117 used as host for plasmids, subcloned in Escherichia coli for site-directed mutagenesis Bacillus subtilis

Protein Variants

EC Number Protein Variants Comment Organism
3.4.24.28 I157D site-directed mutagenesis Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.28 N-[alpha-L-rhamnopyranosyl-oxyhydroxyphosphinyl]-L-leucyl-L-tryptophan
-
 Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.28 0.25
-
 3-(2-furylacryloyl)-L-alanyl-L-phenylalanine amide pH 7.5, 37°C, mutant I157D Bacillus subtilis
3.4.24.28 0.3
-
 3-(2-furylacryloyl)-L-alanyl-L-phenylalanine amide pH 7.5, 37°C, wild-type Bacillus subtilis
3.4.24.28 1.3
-
 3-(2-furylacryloyl)-glycyl-L-leucine amide pH 7.5, 37°C, wild-type Bacillus subtilis
3.4.24.28 1.4
-
 3-(2-furylacryloyl)-glycyl-L-leucine amide pH 7.5, 37°C, mutant I157D Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.28 Bacillus subtilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.28 wild-type and mutant TLPs Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.28 3-(2-furylacryloyl)-glycyl-L-leucine amide + H2O
-
 Bacillus subtilis ?
-
 ?
3.4.24.28 3-(2-furylacryloyl)-L-alanyl-L-phenylalanine amide + H2O
-
 Bacillus subtilis ?
-
 ?
3.4.24.28 beta-casein + H2O
-
 Bacillus subtilis hydrolyzed casein
-
 ?
3.4.24.28 insulin B chain + H2O
-
 Bacillus subtilis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.24.28 thermoysin-like proteinase
-
 Bacillus subtilis
3.4.24.28 TLP-sub
-
 Bacillus subtilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.24.28 58.6
-
 T50 value at pH 7.0 Bacillus subtilis
3.4.24.28 59.5
-
 T50 value at pH 5.3 Bacillus subtilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.24.28 64
-
 3-(2-furylacryloyl)-L-alanyl-L-phenylalanine amide pH 7.5, 37°C, wild-type Bacillus subtilis
3.4.24.28 80
-
 3-(2-furylacryloyl)-glycyl-L-leucine amide pH 7.5, 37°C, mutant I157D Bacillus subtilis
3.4.24.28 99
-
 3-(2-furylacryloyl)-L-alanyl-L-phenylalanine amide pH 7.5, 37°C, mutant I157D Bacillus subtilis
3.4.24.28 256
-
 3-(2-furylacryloyl)-glycyl-L-leucine amide pH 7.5, 37°C, wild-type Bacillus subtilis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.24.28 0.0035
-
 N-[alpha-L-rhamnopyranosyl-oxyhydroxyphosphinyl]-L-leucyl-L-tryptophan pH 7.5, 37°C, wild-type Bacillus subtilis
3.4.24.28 0.0037
-
 N-[alpha-L-rhamnopyranosyl-oxyhydroxyphosphinyl]-L-leucyl-L-tryptophan pH 7.5, 37°C, mutant I157D Bacillus subtilis