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Literature summary extracted from

  • Yamaguchi, F.; Koga, S.; Yoshioka, I.; Takahashi, M.; Sakuraba, H.; Ohshima, T.
    Stable ammonia-specific NAD synthetase from Bacillus stearothermophilus: purification, characterization, gene cloning, and applications (2002), Biosci. Biotechnol. Biochem., 66, 2052-2059.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.1.5 DNA and amino acid sequence determination and analysis, functional overexpression in Escherichia coli Geobacillus stearothermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.1.5 0.91
-
 NH3 pH 8.5, 37°C Geobacillus stearothermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.1.5 Mg2+ required, optimal at 3 mM Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.1.5 25000
-
 2 * 25000, SDS-PAGE Geobacillus stearothermophilus
6.3.1.5 50000
-
 gel filtration Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.1.5 ATP + deamido-NAD+ + NH3 Geobacillus stearothermophilus
-
 AMP + diphosphate + NAD+
-
 ir
6.3.1.5 ATP + deamido-NAD+ + NH3 Geobacillus stearothermophilus H-804
-
 AMP + diphosphate + NAD+
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.5 Geobacillus stearothermophilus E07950 H-804
-
6.3.1.5 Geobacillus stearothermophilus H-804 E07950 H-804
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.1.5 native enzyme to homogeneity Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.1.5 1.59
-
 purified native enzyme Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.5 ATP + deamido-NAD+ + NH3
-
 Geobacillus stearothermophilus AMP + diphosphate + NAD+
-
 ir
6.3.1.5 ATP + deamido-NAD+ + NH3 specific for NH3, no activity with L-glutamine or other L-amino acids, no activity with urea, uric acid, or creatinine Geobacillus stearothermophilus AMP + diphosphate + NAD+
-
 ir
6.3.1.5 ATP + deamido-NAD+ + NH3
-
 Geobacillus stearothermophilus H-804 AMP + diphosphate + NAD+
-
 ir
6.3.1.5 ATP + deamido-NAD+ + NH3 specific for NH3, no activity with L-glutamine or other L-amino acids, no activity with urea, uric acid, or creatinine Geobacillus stearothermophilus H-804 AMP + diphosphate + NAD+
-
 ir

Subunits

EC Number Subunits Comment Organism
6.3.1.5 dimer 2 * 25000, SDS-PAGE Geobacillus stearothermophilus

Synonyms

EC Number Synonyms Comment Organism
6.3.1.5 ammonia-specific NAD synthetase
-
 Geobacillus stearothermophilus
6.3.1.5 NAD synthase
-
 Geobacillus stearothermophilus
6.3.1.5 NAD synthetase
-
 Geobacillus stearothermophilus
6.3.1.5 Nicotinamide adenine dinucleotide synthetase
-
 Geobacillus stearothermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.1.5 60
-
-
 Geobacillus stearothermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.3.1.5 60
-
 stable at Geobacillus stearothermophilus
6.3.1.5 70
-
 40% of the activity at 60°C Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.1.5 9 10
-
 Geobacillus stearothermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.1.5 ATP
-
 Geobacillus stearothermophilus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
6.3.1.5 Geobacillus stearothermophilus isoelectric focusing
-
 5.3