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Literature summary extracted from

  • Yoshida, E.; Arakawa, S.; Matsunaga, T.; Toriumi, S.; Tokuyama, S.; Morikawa, K.; Tahara, Y.
    Cloning, sequencing, and expression of the gene from Bacillus circulans that codes for a heparinase that degrades both heparin and heparan sulfate (2002), Biosci. Biotechnol. Biochem., 66, 1873-1879.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.7 expression in Escherichia coli Niallia circulans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.7 0.0059
-
 heparan sulfate pH 7.5, 40ºC Niallia circulans
4.2.2.7 0.0068
-
 heparin pH 7.5, 40ºC Niallia circulans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.7 Ba2+ about 2fold activation at 5mM Niallia circulans
4.2.2.7 Ca2+ about 2fold activation at 5mM Niallia circulans
4.2.2.7 Co2+ inhibition at 5mM Niallia circulans
4.2.2.7 Cu2+ inhibition at 5mM Niallia circulans
4.2.2.7 Mg2+ about 2fold activation at 5mM Niallia circulans
4.2.2.7 Mn2+ inhibition at 5mM Niallia circulans
4.2.2.7 Ni2+ inhibition at 5mM Niallia circulans
4.2.2.7 Zn2+ inhibition at 5mM Niallia circulans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.7 111200
-
 x * 111200, from amino acid sequence Niallia circulans

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.7 Niallia circulans Q8KKH9 hep gene; HpT298 strain
-
4.2.2.7 Niallia circulans HpT298 Q8KKH9 hep gene; HpT298 strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.7 purification of a recombinant enzyme by ammonium sulfate precipitation followed by DEAE-Sephacel and Sephacryl S-200 column chromatography Niallia circulans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.7 heparan sulfate
-
 Niallia circulans heparan sulfate disaccharides with 4-deoxy-alpha-D-gluc-4-enuronosyl groups at their non-reducing ends
-
 ?
4.2.2.7 heparan sulfate
-
 Niallia circulans heparan sulfate disaccharides with 4-deoxy-alpha-D-gluc-4-enuronosyl groups at their non-reducing ends several disaccharides formed by 4-deoxy-alpha-L-threo-hex-4-enepyranosyluronic acid and glucosamine, both free and sulfated ?
4.2.2.7 heparan sulfate
-
 Niallia circulans HpT298 heparan sulfate disaccharides with 4-deoxy-alpha-D-gluc-4-enuronosyl groups at their non-reducing ends
-
 ?
4.2.2.7 heparan sulfate
-
 Niallia circulans HpT298 heparan sulfate disaccharides with 4-deoxy-alpha-D-gluc-4-enuronosyl groups at their non-reducing ends several disaccharides formed by 4-deoxy-alpha-L-threo-hex-4-enepyranosyluronic acid and glucosamine, both free and sulfated ?
4.2.2.7 heparin
-
 Niallia circulans unsaturated heparin disaccharide + ?
-
 ?
4.2.2.7 heparin
-
 Niallia circulans unsaturated heparin disaccharide + ? several disaccharides formed by 4-deoxy-alpha-L-threo-hex-4-enepyranosyluronic acid and glucosamine, both free and sulfated ?
4.2.2.7 heparin
-
 Niallia circulans HpT298 unsaturated heparin disaccharide + ?
-
 ?
4.2.2.7 heparin
-
 Niallia circulans HpT298 unsaturated heparin disaccharide + ? several disaccharides formed by 4-deoxy-alpha-L-threo-hex-4-enepyranosyluronic acid and glucosamine, both free and sulfated ?
4.2.2.7 additional information no catalytic activity on dermatan sulfate and keratan sulfate as substrates Niallia circulans ?
-
 ?
4.2.2.7 additional information no catalytic activity on dermatan sulfate and keratan sulfate as substrates Niallia circulans HpT298 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.7 ? x * 111200, from amino acid sequence Niallia circulans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.7 40
-
 pH 7.5 Niallia circulans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.7 50
-
 60% of the maximal activity retained after 1h Niallia circulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.7 7.5
-
-
 Niallia circulans