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Literature summary extracted from

  • Yonezawa, H.; Kaizuka, H.; Uchikoba, T.; Arima, K.; Kaneda, M.
    Substrate specificity of cucumisin on synthetic peptides (2000), Biosci. Biotechnol. Biochem., 64, 2104-2108.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.25 0.6
-
 Leu-Leu-Pro-Ser-Ala-Leu pH 8.0, 37°C Cucumis melo
3.4.21.25 0.6
-
 Leu-Leu-Pro-Phe-Ala-Leu pH 8.0, 37°C Cucumis melo
3.4.21.25 0.7
-
 Leu-Leu-Pro-Leu-Ala-Leu pH 8.0, 37°C Cucumis melo
3.4.21.25 0.8
-
 Leu-Leu-Pro-Glu-Ser-Leu pH 8.0, 37°C Cucumis melo
3.4.21.25 1.1
-
 Leu-Leu-Pro-Thr-Ala-Leu pH 8.0, 37°C Cucumis melo
3.4.21.25 1.8
-
 Leu-Leu-Pro-Ala-Ala-Leu pH 8.0, 37°C Cucumis melo
3.4.21.25 2.8
-
 Leu-Leu-Pro-Glu-Ala-Leu pH 8.0, 37°C Cucumis melo
3.4.21.25 2.9
-
 Leu-Leu-Pro-His-Ala-Leu pH 8.0, 37°C Cucumis melo
3.4.21.25 3
-
 Leu-Leu-Pro-Glu-Lys-Leu pH 8.0, 37°C Cucumis melo
3.4.21.25 3.9
-
 Leu-Leu-Pro-Asn-Ala-Leu pH 8.0, 37°C Cucumis melo
3.4.21.25 4.1
-
 Leu-Leu-Pro-Met-Ala-Leu pH 8.0, 37°C Cucumis melo
3.4.21.25 5.6
-
 Leu-Leu-Pro-Gln-Ala-Leu pH 8.0, 37°C Cucumis melo

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.25 Cucumis melo
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.25 Leu-Leu-Leu-Leu-Pro-Glu-Ala-Leu + H2O
-
 Cucumis melo Leu-Leu-Leu-Leu-Pro-Glu + Ala-Leu
-
 ?
3.4.21.25 Leu-Leu-Leu-Pro-Glu-Ala-Leu + H2O
-
 Cucumis melo Leu-Leu-Leu-Pro-Glu + Ala-Leu
-
 ?
3.4.21.25 Leu-Leu-Pro-Ala-Ala-Leu + H2O
-
 Cucumis melo Leu-Leu-Pro-Ala + Ala-Leu
-
 ?
3.4.21.25 Leu-Leu-Pro-Arg-Ala-Leu + H2O low activity Cucumis melo Leu-Leu-Pro-Arg + Ala-Leu
-
 ?
3.4.21.25 Leu-Leu-Pro-Asn-Ala-Leu + H2O
-
 Cucumis melo Leu-Leu-Pro-Asn + Ala-Leu
-
 ?
3.4.21.25 Leu-Leu-Pro-Asp-Ala-Leu + H2O
-
 Cucumis melo Leu-Leu-Pro-Asp + Ala-Leu
-
 ?
3.4.21.25 Leu-Leu-Pro-Gln-Ala-Leu + H2O
-
 Cucumis melo Leu-Leu-Pro-Gln + Ala-Leu
-
 ?
3.4.21.25 Leu-Leu-Pro-Glu-Ala + H2O low activity Cucumis melo Leu-Leu-Pro-Glu + Ala
-
 ?
3.4.21.25 Leu-Leu-Pro-Glu-Ala-Leu + H2O low activity Cucumis melo Leu-Leu-Pro-Glu + Ala-Leu
-
 ?
3.4.21.25 Leu-Leu-Pro-Glu-Ala-Leu-Leu + H2O
-
 Cucumis melo Leu-Leu-Pro-Glu + Ala-Leu-Leu
-
 ?
3.4.21.25 Leu-Leu-Pro-Glu-Ala-Leu-Leu-Leu + H2O
-
 Cucumis melo Leu-Leu-Pro-Glu + Ala-Leu-Leu-Leu
-
 ?
3.4.21.25 Leu-Leu-Pro-Glu-Glu-Leu + H2O low activity Cucumis melo Leu-Leu-Pro-Glu + Glu-Leu
-
 ?
3.4.21.25 Leu-Leu-Pro-Glu-Gly-Leu + H2O
-
 Cucumis melo Leu-Leu-Pro-Glu + Gly-Leu
-
 ?
3.4.21.25 Leu-Leu-Pro-Glu-Lys-Leu + H2O
-
 Cucumis melo Leu-Leu-Pro-Glu + Lys-Leu
-
 ?
3.4.21.25 Leu-Leu-Pro-Glu-Phe-Leu + H2O low activity Cucumis melo Leu-Leu-Pro-Glu + Phe-Leu
-
 ?
3.4.21.25 Leu-Leu-Pro-Glu-Ser-Leu + H2O
-
 Cucumis melo Leu-Leu-Pro-Glu + Ser-Leu
-
 ?
3.4.21.25 Leu-Leu-Pro-His-Ala-Leu + H2O
-
 Cucumis melo Leu-Leu-Pro-His + Ala-Leu
-
 ?
3.4.21.25 Leu-Leu-Pro-Leu-Ala-Leu + H2O
-
 Cucumis melo Leu-Leu-Pro-Leu + Ala-Leu
-
 ?
3.4.21.25 Leu-Leu-Pro-Lys-Ala-Leu + H2O low activity Cucumis melo Leu-Leu-Pro-Lys + Ala-Leu
-
 ?
3.4.21.25 Leu-Leu-Pro-Met-Ala-Leu + H2O best substrate Cucumis melo Leu-Leu-Pro-Met + Ala-Leu
-
 ?
3.4.21.25 Leu-Leu-Pro-Phe-Ala-Leu + H2O
-
 Cucumis melo Leu-Leu-Pro-Phe + Ala-Leu
-
 ?
3.4.21.25 Leu-Leu-Pro-Ser-Ala-Leu + H2O
-
 Cucumis melo Leu-Leu-Pro-Ser + Ala-Leu
-
 ?
3.4.21.25 Leu-Leu-Pro-Thr-Ala-Leu + H2O
-
 Cucumis melo Leu-Leu-Pro-Thr + Ala-Leu
-
 ?
3.4.21.25 Leu-Leu-Pro-Trp-Ala-Leu + H2O
-
 Cucumis melo Leu-Leu-Pro-Trp + Ala-Leu
-
 ?
3.4.21.25 Leu-Pro-Glu-Ala-Leu + H2O low activity Cucumis melo Leu-Pro-Glu + Ala-Leu
-
 ?
3.4.21.25 additional information substrate specificity at positions P6-P'4, no activity with Leu-Leu-Pro-Glu-Val-Leu, Pro-Glu-Ala-Leu, Leu-Pro-Glu-Ala, Leu-Leu-Pro-Gly-Ala-Leu, Leu-Leu-Pro-Ile-Ala-Leu and Leu-Leu-Pro-Pro-Ala-Leu, poor substrates are Leu-Leu-Pro-Tyr-Ala-Leu, Leu-Leu-Pro-Glu-Leu-Leu and Leu-Leu-Pro-Val-Ala-Leu Cucumis melo ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.25 More enzyme belongs to peptidase family S8 Cucumis melo

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.25 37
-
 assay at Cucumis melo

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.25 3
-
 Leu-Leu-Pro-Ser-Ala-Leu pH 8.0, 37°C Cucumis melo
3.4.21.25 4
-
 Leu-Leu-Pro-Glu-Lys-Leu pH 8.0, 37°C Cucumis melo
3.4.21.25 5
-
 Leu-Leu-Pro-Phe-Ala-Leu pH 8.0, 37°C Cucumis melo
3.4.21.25 5
-
 Leu-Leu-Pro-Glu-Ala-Leu pH 8.0, 37°C Cucumis melo
3.4.21.25 7
-
 Leu-Leu-Pro-Glu-Ser-Leu pH 8.0, 37°C Cucumis melo
3.4.21.25 14
-
 Leu-Leu-Pro-Ala-Ala-Leu pH 8.0, 37°C Cucumis melo
3.4.21.25 20
-
 Leu-Leu-Pro-Leu-Ala-Leu pH 8.0, 37°C Cucumis melo
3.4.21.25 27
-
 Leu-Leu-Pro-Thr-Ala-Leu pH 8.0, 37°C Cucumis melo
3.4.21.25 59
-
 Leu-Leu-Pro-His-Ala-Leu pH 8.0, 37°C Cucumis melo
3.4.21.25 98
-
 Leu-Leu-Pro-Asn-Ala-Leu pH 8.0, 37°C Cucumis melo
3.4.21.25 162
-
 Leu-Leu-Pro-Gln-Ala-Leu pH 8.0, 37°C Cucumis melo
3.4.21.25 177
-
 Leu-Leu-Pro-Met-Ala-Leu pH 8.0, 37°C Cucumis melo

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.25 8
-
 assay at Cucumis melo