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Literature summary extracted from

  • Hatakeyama, K.; Goto, M.; Kobayashi, M.; Terasawa, M.; Yukawa, H.
    Analysis of oxidation sensitivity of maleate cis-trans isomerase from Serratia marcescens (2000), Biosci. Biotechnol. Biochem., 64, 1477-1485.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.2.1.1 expression in Escherichia coli Serratia marcescens

Protein Variants

EC Number Protein Variants Comment Organism
5.2.1.1 M138C mutant enzyme shows 90% of the activity of the wild-type enzyme Serratia marcescens
5.2.1.1 M18C mutant enzyme shows 30% of the activity of the wild-type enzyme Serratia marcescens
5.2.1.1 M201C mutant enzyme shows 90% of the activity of the wild-type enzyme Serratia marcescens
5.2.1.1 M46C mutant enzyme shows 30% of the activity of the wild-type enzyme Serratia marcescens
5.2.1.1 M57C mutant enzyme shows 40% of the activity of the wild-type enzyme Serratia marcescens
5.2.1.1 M85C mutant enzyme shows 90% of the activity of the wild-type enzyme Serratia marcescens

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.2.1.1 H2O2 1 mM, complete inhibition, Met201 is one of the targeted sites for the inactivation by chemical oxidation Serratia marcescens
5.2.1.1 iodoacetamide 1 mM, 72% inhibition Serratia marcescens
5.2.1.1 N-bromosuccinimide 1 mM, complete inhibition Serratia marcescens
5.2.1.1 N-Chlorosuccinimide 1 mM, complete inhibition Serratia marcescens
5.2.1.1 p-toluenesulfonchloramide 1 mM, complete inhibition Serratia marcescens
5.2.1.1 PCMB 0.1 mM, complete inhibition Serratia marcescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.2.1.1 26950
-
 x * 26950, calculation from nucleotide sequence Serratia marcescens
5.2.1.1 28000
-
 x * 28000, SDS-PAGE Serratia marcescens

Organism

EC Number Organism UniProt Comment Textmining
5.2.1.1 Alcaligenes faecalis
-
-
-
5.2.1.1 Geobacillus stearothermophilus
-
-
-
5.2.1.1 Serratia marcescens
-
 IFO3736
-
5.2.1.1 Serratia marcescens IFO3736
-
 IFO3736
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.2.1.1
-
 Serratia marcescens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.2.1.1 20
-
-
 Serratia marcescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.2.1.1 Maleate
-
 Alcaligenes faecalis Fumarate
-
 ?
5.2.1.1 Maleate
-
 Geobacillus stearothermophilus Fumarate
-
 ?
5.2.1.1 Maleate
-
 Serratia marcescens Fumarate
-
 ?
5.2.1.1 Maleate
-
 Serratia marcescens IFO3736 Fumarate
-
 ?

Subunits

EC Number Subunits Comment Organism
5.2.1.1 ? x * 28000, SDS-PAGE Serratia marcescens
5.2.1.1 ? x * 26950, calculation from nucleotide sequence Serratia marcescens

Synonyms

EC Number Synonyms Comment Organism
5.2.1.1 MaiA
-
 Serratia marcescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.2.1.1 45
-
-
 Alcaligenes faecalis
5.2.1.1 45
-
-
 Serratia marcescens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
5.2.1.1 30 70 increase of activity from 30°C to 70°C Geobacillus stearothermophilus
5.2.1.1 30 60 30°C: about 60% of maximal activity, 60°C: about 40% of maximal activity Alcaligenes faecalis
5.2.1.1 30 60 30°C: about 70% of maximal activity, 60°C: about 70% of maximal activity Serratia marcescens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.2.1.1 40
-
 30 min, stable Alcaligenes faecalis
5.2.1.1 45
-
 30 min, about 10% loss of activity Alcaligenes faecalis
5.2.1.1 50
-
 30 min, about 45% loss of activity Alcaligenes faecalis
5.2.1.1 53
-
 30 min, complete inactivation Alcaligenes faecalis
5.2.1.1 57
-
 30 min, stable below Geobacillus stearothermophilus
5.2.1.1 60
-
 30 min, about 25% loss of activity Geobacillus stearothermophilus
5.2.1.1 60
-
 30 min, retains almost 60% of the initial activity Serratia marcescens