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Literature summary extracted from

  • Tanaka, T.; Matsuzawa, H.; Ohta, T.
    Substrate specificity of aqualysin I, a bacterial thermophilic alkaline serine protease from Thermus aquaticus YT-1: comparison with proteinase K, subtilisin BPN' and subtilisin Carlsberg (1998), Biosci. Biotechnol. Biochem., 62, 2161-2165.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.111 0.036
-
 N-succinyl-Phe-Nle-Ala-4-nitroanilide pH 7.4, 40°C Thermus aquaticus
3.4.21.111 0.039
-
 N-succinyl-Phe-Val-Ala-4-nitroanilide pH 7.4, 40°C Thermus aquaticus
3.4.21.111 0.04
-
 N-succinyl-Phe-Ala-Ala-4-nitroanilide pH 7.4, 40°C Thermus aquaticus
3.4.21.111 0.064
-
 N-succinyl-Ile-Val-Ala-4-nitroanilide pH 7.4, 40°C Thermus aquaticus
3.4.21.111 0.087
-
 N-succinyl-Phe-Leu-Ala-4-nitroanilide pH 7.4, 40°C Thermus aquaticus
3.4.21.111 0.41
-
 N-succinyl-Phe-Val-Leu-4-nitroanilide pH 7.4, 40°C Thermus aquaticus
3.4.21.111 0.79
-
 N-succinyl-Ala-Pro-Ala-4-nitroanilide pH 7.4, 40°C Thermus aquaticus
3.4.21.111 0.91
-
 N-succinyl-Ala-Ala-Phe-4-nitroanilide pH 7.4, 40°C Thermus aquaticus
3.4.21.111 1.2
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.4, 40°C Thermus aquaticus
3.4.21.111 1.3
-
 N-succinyl-Ala-Ala-Ala-4-nitroanilide pH 7.4, 40°C Thermus aquaticus
3.4.21.111 1.6
-
 N-succinyl-Gly-Gly-Phe-4-nitroanilide pH 7.4, 40°C Thermus aquaticus
3.4.21.111 1.6
-
 N-succinyl-Ala-Ala-Val-4-nitroanilide pH 7.4, 40°C Thermus aquaticus

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.111 Thermus aquaticus
-
 YT-1
-
3.4.21.111 Thermus aquaticus YT-1
-
 YT-1
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.111 additional information enzyme has 3 subsites S1, S2 and S3 in its substrate binding site. S1 site prefers alanine and phenylalanine, S2 site prefers alanine and norleucine, S3 site prefers phenylalanine and isoleucine Thermus aquaticus ?
-
 ?
3.4.21.111 additional information enzyme has 3 subsites S1, S2 and S3 in its substrate binding site. S1 site prefers alanine and phenylalanine, S2 site prefers alanine and norleucine, S3 site prefers phenylalanine and isoleucine Thermus aquaticus YT-1 ?
-
 ?
3.4.21.111 N-succinyl-Ala-Ala-Ala-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Ala-Ala-Ala + 4-nitroaniline
-
 ?
3.4.21.111 N-succinyl-Ala-Ala-Ala-4-nitroanilide + H2O
-
 Thermus aquaticus YT-1 N-succinyl-Ala-Ala-Ala + 4-nitroaniline
-
 ?
3.4.21.111 N-succinyl-Ala-Ala-Phe-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Ala-Ala-Phe + 4-nitroaniline
-
 ?
3.4.21.111 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
 ?
3.4.21.111 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide + H2O
-
 Thermus aquaticus YT-1 N-succinyl-Ala-Ala-Pro-Phe + 4-nitroaniline
-
 ?
3.4.21.111 N-succinyl-Ala-Ala-Val-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Ala-Ala-Val + 4-nitroaniline
-
 ?
3.4.21.111 N-succinyl-Ala-Pro-Ala-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Ala-Pro-Ala + 4-nitroaniline
-
 ?
3.4.21.111 N-succinyl-Gly-betaAla-Ala-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Gly-betaAla-Ala + 4-nitroaniline
-
 ?
3.4.21.111 N-succinyl-Gly-Gly-Phe-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Gly-Gly-Phe + 4-nitroaniline
-
 ?
3.4.21.111 N-succinyl-Ile-betaAla-Ala-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Ile-betaAla-Ala + 4-nitroaniline
-
 ?
3.4.21.111 N-succinyl-Ile-Val-Ala-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Ile-Val-Ala + 4-nitroaniline
-
 ?
3.4.21.111 N-succinyl-Leu-betaAla-Ala-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Leu-betaAla-Ala + 4-nitroaniline
-
 ?
3.4.21.111 N-succinyl-Phe-Ala-Ala-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Phe-Ala-Ala + 4-nitroaniline
-
 ?
3.4.21.111 N-succinyl-Phe-Ala-Ala-4-nitroanilide + H2O
-
 Thermus aquaticus YT-1 N-succinyl-Phe-Ala-Ala + 4-nitroaniline
-
 ?
3.4.21.111 N-succinyl-Phe-betaAla-Ala-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Phe-betaAla-Ala + 4-nitroaniline
-
 ?
3.4.21.111 N-succinyl-Phe-Leu-Ala-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Phe-Leu-Ala + 4-nitroaniline
-
 ?
3.4.21.111 N-succinyl-Phe-Nle-Ala-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Phe-Nle-Ala + 4-nitroaniline
-
 ?
3.4.21.111 N-succinyl-Phe-Nle-Ala-4-nitroanilide + H2O
-
 Thermus aquaticus YT-1 N-succinyl-Phe-Nle-Ala + 4-nitroaniline
-
 ?
3.4.21.111 N-succinyl-Phe-Val-Ala-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Phe-Val-Ala + 4-nitroaniline
-
 ?
3.4.21.111 N-succinyl-Phe-Val-Leu-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Phe-Val-Leu + 4-nitroaniline
-
 ?
3.4.21.111 N-succinyl-Phe-Val-Phe-4-nitroanilide + H2O
-
 Thermus aquaticus N-succinyl-Phe-Val-Phe + 4-nitroaniline
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.111 0.047
-
 N-succinyl-Ala-Ala-Val-4-nitroanilide pH 7.4, 40°C Thermus aquaticus
3.4.21.111 0.069
-
 N-succinyl-Phe-Val-Leu-4-nitroanilide pH 7.4, 40°C Thermus aquaticus
3.4.21.111 0.1
-
 N-succinyl-Phe-Ala-Ala-4-nitroanilide pH 7.4, 40°C Thermus aquaticus
3.4.21.111 0.15
-
 N-succinyl-Gly-Gly-Phe-4-nitroanilide pH 7.4, 40°C Thermus aquaticus
3.4.21.111 0.48
-
 N-succinyl-Ala-Pro-Ala-4-nitroanilide pH 7.4, 40°C Thermus aquaticus
3.4.21.111 1.4
-
 N-succinyl-Ala-Ala-Ala-4-nitroanilide pH 7.4, 40°C Thermus aquaticus
3.4.21.111 2.5
-
 N-succinyl-Ala-Ala-Phe-4-nitroanilide pH 7.4, 40°C Thermus aquaticus
3.4.21.111 3.3
-
 N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide pH 7.4, 40°C Thermus aquaticus
3.4.21.111 3.6
-
 N-succinyl-Phe-Leu-Ala-4-nitroanilide pH 7.4, 40°C Thermus aquaticus
3.4.21.111 5
-
 N-succinyl-Phe-Val-Phe-4-nitroanilide pH 7.4, 40°C Thermus aquaticus
3.4.21.111 8.8
-
 N-succinyl-Phe-Nle-Ala-4-nitroanilide pH 7.4, 40°C Thermus aquaticus