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Literature summary extracted from

  • Ryan, M.; Zaikova, T.O.; Keana, J.F.W.; Goldfine, H.; Griffith, O.H.
    Listeria monocytogenes phosphatidylinositol-specific phospholipase C: Activation and allostery (2002), Biophys. Chem., 101-102, 347-358.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.6.1.13 dihexanoyl phosphatidylcholine activates, 4-5fold increase in catalytic efficiency, binds to a lipid-binding subsite, not to the active site, maximal activation at 0.4 mM Listeria monocytogenes

Application

EC Number Application Comment Organism
4.6.1.13 medicine PI-PLC is a virulence factor of the animal and human pathogen causing listeriosis Listeria monocytogenes

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.6.1.13 PI-PLC gene is part of the virulence gene cluster, overexpression in Escherichia coli MM294 Listeria monocytogenes

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.6.1.13 recombinant PI-PLC Listeria monocytogenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.6.1.13 additional information
-
 additional information kinetics, two-site kinetic model Listeria monocytogenes
4.6.1.13 0.061
-
 methyl-fluorescein myo-inositol phosphate pH 7 Listeria monocytogenes

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.6.1.13 Mg2+ MgCl2, activates Listeria monocytogenes
4.6.1.13 additional information salts have an activity-enhancing effect Listeria monocytogenes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.6.1.13 32900
-
-
 Listeria monocytogenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.6.1.13 1-phosphatidyl-1D-myo-inositol Listeria monocytogenes natural aggregate substrate, PI-PLC is a virulence factor of the animal and human pathogen 1D-myo-inositol 1,2-cyclic phosphate + diacylglycerol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.13 Listeria monocytogenes
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.6.1.13 recombinant PI-PLC Listeria monocytogenes

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.6.1.13 30
-
 pH 7.2, phosphatidylinositol as substrate, in absence of MgCl2 Listeria monocytogenes
4.6.1.13 700 1300 pH 7.2, phosphatidylinositol as substrate, in presence of MgCl2 Listeria monocytogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.13 1-phosphatidyl-1D-myo-inositol natural aggregate substrate, PI-PLC is a virulence factor of the animal and human pathogen Listeria monocytogenes 1D-myo-inositol 1,2-cyclic phosphate + diacylglycerol
-
 ?
4.6.1.13 1-phosphatidyl-1D-myo-inositol natural aggregate substrate, two-site enzyme model with interfacial cooperativity between the active site and a lipid-binding subsite, presumably adjacent to the active site Listeria monocytogenes 1D-myo-inositol 1,2-cyclic phosphate + sn-1,2-diacylglycerol
-
 ?
4.6.1.13 methyl-fluorescein myo-inositol phosphate monomeric substrate, only the D-enantiomer is active Listeria monocytogenes D-myo-inositol 1,2-cyclic phosphate + methyl-fluorescein
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.6.1.13 66
-
 methyl-fluorescein myo-inositol phosphate pH 7 Listeria monocytogenes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.6.1.13 7 7.2 assay at Listeria monocytogenes