Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Dodt, J.; Reichwein, J.
    Human cathepsin H: deletion of the mini-chain switches substrate specificity from aminopeptidase to endopeptidase (2003), Biol. Chem., 384, 1327-1332.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.4.22.16 additional information mutant lacking the mini-chain, T11_E18del-cathepsin H, minimal aminopeptidase activity against good wild type substrates, endopeptidase activity against Z-L-Phe-L-Arg-NH-4-methylcoumaryl-7-amide, which is not cleaved by wild type Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.16 chicken egg white cystatin
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.16 0.05
-
 H-L-Arg-NH-4-methylcoumaryl-7-amide wild type enzyme, pH 6.8, 30°C Homo sapiens
3.4.22.16 0.092
-
 Z-L-Phe-L-Arg-NH-4-methylcoumaryl-7-amide mutant T11_E18del-cathepsin H, pH 6.8, 30°C Homo sapiens
3.4.22.16 3.6
-
 H-L-Arg-NH-4-methylcoumaryl-7-amide mutant T11_E18del-cathepsin H, pH 6.8, 30°C Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.16 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.16 Arg-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens L-arginine + 7-amino-4-methylcoumarin
-
 ?
3.4.22.16 Z-L-Phe-L-Arg-4-methylcoumaryl-7-amide + H2O no substrate for wild type enzyme, endopeptidase activity of mutant T11_E18del-cathepsin H Homo sapiens ?
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.22.16 0.000000095
-
 chicken egg white cystatin mutant T11_E18del-cathepsin H, pH 6.8, 30°C Homo sapiens
3.4.22.16 0.00000053
-
 chicken egg white cystatin wild type enzyme, pH 6.8, 30°C Homo sapiens