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Literature summary extracted from
- A single amino acid substitution can shift the optimum pH of DNase I for enzyme activity: biochemical and molecular analysis of the piscine DNase I family (2004), Biochim. Biophys. Acta, 1672, 174-183.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.21.1 | DNA and amino acid sequence determination and analysis, expression in COS-7 cells | Cyprinus carpio |
| 3.1.21.1 | DNA and amino acid sequence determination and analysis, expression in COS-7 cells | Pagrus major |
| 3.1.21.1 | DNA and amino acid sequence determination and analysis, expression of wild-type and mutant enzymes in COS-7 cells | Anguilla japonica |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.1.21.1 | D44H | shifts the pH optimum from pH 8.0 to lower values of pH 6.5-7.0, 70% reduced activity compared to thw wild-type enzyme | Anguilla japonica |
| 3.1.21.1 | E190S | pH profile remains similar to the wild-type enzyme with an optimum around pH 8.0 | Anguilla japonica |
| 3.1.21.1 | M118R | pH profile remains similar to the wild-type enzyme with an optimum around pH 8.0 | Anguilla japonica |
| 3.1.21.1 | M236Q | pH profile remains similar to the wild-type enzyme with an optimum around pH 8.0 | Anguilla japonica |
| 3.1.21.1 | Q134L | pH profile remains similar to the wild-type enzyme with an optimum around pH 8.0 | Anguilla japonica |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.21.1 | Ca2+ | activates | Cyprinus carpio |  |
| 3.1.21.1 | Ca2+ | activates | Anguilla japonica | |
| 3.1.21.1 | Ca2+ | activates | Pagrus major | |
| 3.1.21.1 | Ca2+ | activates | Oreochromis mossambicus | |
| 3.1.21.1 | Ca2+ | activates | Takifugu rubripes | |
| 3.1.21.1 | Mg2+ | activates | Cyprinus carpio | |
| 3.1.21.1 | Mg2+ | activates | Anguilla japonica | |
| 3.1.21.1 | Mg2+ | activates | Pagrus major | |
| 3.1.21.1 | Mg2+ | activates | Oreochromis mossambicus | |
| 3.1.21.1 | Mg2+ | activates | Takifugu rubripes | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.21.1 | Anguilla japonica | Q5KTT1 | eel | - |
| 3.1.21.1 | Cyprinus carpio | Q8JIP7 | carp | - |
| 3.1.21.1 | Oreochromis mossambicus | O42446 | tilapia | - |
| 3.1.21.1 | Pagrus major | Q5KTT0 | sea bream | - |
| 3.1.21.1 | Takifugu rubripes | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.21.1 | 10000fold from hepatopancreas | Pagrus major |
| 3.1.21.1 | 10000fold from pancreas | Anguilla japonica |
| 3.1.21.1 | 14000fold from hepatopancreas | Oreochromis mossambicus |
| 3.1.21.1 | 17000fold from hepatopancreas | Cyprinus carpio |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 3.1.21.1 | endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products | Asp44 is involved in catalysis, while Met118, Gln134, Glu190, and Met236 are not | Anguilla japonica | |
| 3.1.21.1 | endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products | preference for double-stranded DNA | Cyprinus carpio | |
| 3.1.21.1 | endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products | preference for double-stranded DNA | Pagrus major | |
| 3.1.21.1 | endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products | preference for double-stranded DNA | Oreochromis mossambicus | |
| 3.1.21.1 | endonucleolytic cleavage to 5'-phosphodinucleotide and 5'-phosphooligonucleotide end-products | preference for double-stranded DNA | Takifugu rubripes |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.21.1 | hepatopancreas | - |
Cyprinus carpio | - |
| 3.1.21.1 | hepatopancreas | - |
Pagrus major | - |
| 3.1.21.1 | hepatopancreas | - |
Oreochromis mossambicus | - |
| 3.1.21.1 | pancreas | - |
Anguilla japonica | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.21.1 | additional information | - |
purified enzyme | Cyprinus carpio |
| 3.1.21.1 | additional information | - |
purified enzyme | Pagrus major |
| 3.1.21.1 | additional information | - |
purified enzyme | Oreochromis mossambicus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.21.1 | DNase I | - |
Cyprinus carpio |
| 3.1.21.1 | DNase I | - |
Anguilla japonica |
| 3.1.21.1 | DNase I | - |
Pagrus major |
| 3.1.21.1 | DNase I | - |
Oreochromis mossambicus |
| 3.1.21.1 | DNase I | - |
Takifugu rubripes |
| 3.1.21.1 | More | enzyme belongs to the piscine DNase I family | Cyprinus carpio |
| 3.1.21.1 | More | enzyme belongs to the piscine DNase I family | Anguilla japonica |
| 3.1.21.1 | More | enzyme belongs to the piscine DNase I family | Pagrus major |
| 3.1.21.1 | More | enzyme belongs to the piscine DNase I family | Oreochromis mossambicus |
| 3.1.21.1 | More | enzyme belongs to the piscine DNase I family | Takifugu rubripes |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.21.1 | 37 | - |
assay at | Cyprinus carpio |
| 3.1.21.1 | 37 | - |
assay at | Anguilla japonica |
| 3.1.21.1 | 37 | - |
assay at | Pagrus major |
| 3.1.21.1 | 37 | - |
assay at | Oreochromis mossambicus |
| 3.1.21.1 | 37 | - |
assay at | Takifugu rubripes |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.21.1 | 6 | 8 | Asp44 is responsible for the higher pH optimum compared to vertebrates, residue 44 might have been involved in the evolutionary change of pH optimum for activity from piscine, reptile and amphibia to vertebrates, who possess a His44 residue and a lower pH optimum | Pagrus major |
| 3.1.21.1 | 8 | - |
about, Asp44 is responsible for the higher pH optimum compared to vertebrates, residue 44 might have been involved in the evolutionary change of pH optimum for activity from piscine, reptile and amphibia to vertebrates, who possess a His44 residue and a lower pH optimum | Cyprinus carpio |
| 3.1.21.1 | 8 | - |
about, Asp44 is responsible for the higher pH optimum compared to vertebrates, residue 44 might have been involved in the evolutionary change of pH optimum for activity from piscine, reptile and amphibia to vertebrates, who possess a His44 residue and a lower pH optimum | Anguilla japonica |
| 3.1.21.1 | 8 | - |
about, Asp44 is responsible for the higher pH optimum compared to vertebrates, residue 44 might have been involved in the evolutionary change of pH optimum for activity from piscine, reptile and amphibia to vertebrates, who possess a His44 residue and a lower pH optimum | Oreochromis mossambicus |
| 3.1.21.1 | 8 | - |
about, Asp44 is responsible for the higher pH optimum compared to vertebrates, residue 44 might have been involved in the evolutionary change of pH optimum for activity from piscine, reptile and amphibia to vertebrates, who possess a His44 residue and a lower pH optimum | Takifugu rubripes |
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