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Literature summary extracted from

  • Sawada, K.; Suzumatsu, A.; Kobayashi, T.; Ito, S.
    Molecular cloning and sequencing of the gene encoding an exopolygalacturonase of a Bacillus isolate and properties of its recombinant enzyme (2001), Biochim. Biophys. Acta, 1568, 162-170.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.82
-
 Bacillus sp. (in: Bacteria)

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.82 Ca2+ 0.4 mM Ca2+ shifts the optimal temperature from 50°C to 55°C, the activity is 1.2fold higher than the maximal activity without Ca2+ at 50°C Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.82 105000
-
 x * 105000, SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.82 Bacillus sp. (in: Bacteria) Q8RR74
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.82
-
 Bacillus sp. (in: Bacteria)

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.82 culture medium
-
 Bacillus sp. (in: Bacteria)
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.82 33.1
-
-
 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.82 additional information the enzyme is unable to degrade citrus pectins with degrees of esterification of 63% and 93% Bacillus sp. (in: Bacteria) ?
-
 ?
3.2.1.82 oligogalacturonate + H2O
-
 Bacillus sp. (in: Bacteria) digalacturonate
-
 ?
3.2.1.82 polygalacturonate + H2O
-
 Bacillus sp. (in: Bacteria) digalacturonate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.82 ? x * 105000, SDS-PAGE Bacillus sp. (in: Bacteria)

Synonyms

EC Number Synonyms Comment Organism
3.2.1.82 exo-PHase
-
 Bacillus sp. (in: Bacteria)
3.2.1.82 exopolygalacturonase
-
 Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.82 55
-
 Tris-HCl buffer in presence of Ca2+ Bacillus sp. (in: Bacteria)

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.82 30 65 30°C: about 30% of maximal activity, 65°C: about 30% of maximal activity Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.82 50
-
 pH 7.0, 15 min, stable up to Bacillus sp. (in: Bacteria)
3.2.1.82 60
-
 pH 7.0, 15 min, about 5% loss of activity Bacillus sp. (in: Bacteria)
3.2.1.82 65
-
 pH 7.0, 15 min, about 90% loss of activity Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.82 8
-
-
 Bacillus sp. (in: Bacteria)

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.82 7 10 pH 7.0: about 55% of maximal activity, pH 10.0: about 35% of maximal activity Bacillus sp. (in: Bacteria)

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.82 Bacillus sp. (in: Bacteria) isoelectrofocusing, pH-gradient 3.5-9.5
-
 5