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Literature summary extracted from

  • Soh, B.S.; Loke, P.; Sim, T.S.
    Cloning, heterologous expression and purification of an isocitrate lyase from Streptomyces clavuligerus NRRL 3585 (2001), Biochim. Biophys. Acta, 1522, 112-117.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.3.1 expressed in Escherichia coli Streptomyces clavuligerus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.1 475000
-
 calculated from amino acid sequence Streptomyces clavuligerus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.3.1 isocitrate Streptomyces clavuligerus
-
 succinate + glyoxylate
-
 ?
4.1.3.1 isocitrate Streptomyces clavuligerus NRRL 3585
-
 succinate + glyoxylate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.1 Streptomyces clavuligerus Q8RPZ0 NRRL 3585
-
4.1.3.1 Streptomyces clavuligerus NRRL 3585 Q8RPZ0 NRRL 3585
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.1 90% homogeneity Streptomyces clavuligerus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.3.1 2.23
-
-
 Streptomyces clavuligerus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.1 isocitrate
-
 Streptomyces clavuligerus succinate + glyoxylate
-
 ?
4.1.3.1 isocitrate
-
 Streptomyces clavuligerus NRRL 3585 succinate + glyoxylate
-
 ?