BRENDA - Enzyme Database

Cloning, heterologous expression and purification of an isocitrate lyase from Streptomyces clavuligerus NRRL 3585

Soh, B.S.; Loke, P.; Sim, T.S.; Biochim. Biophys. Acta 1522, 112-117 (2001)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.1.3.1
expressed in Escherichia coli
Streptomyces clavuligerus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
475000
-
calculated from amino acid sequence
Streptomyces clavuligerus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Streptomyces clavuligerus
-
succinate + glyoxylate
-
Streptomyces clavuligerus
?
4.1.3.1
isocitrate
Streptomyces clavuligerus NRRL 3585
-
succinate + glyoxylate
-
Streptomyces clavuligerus NRRL 3585
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.3.1
Streptomyces clavuligerus
Q8RPZ0
NRRL 3585
-
4.1.3.1
Streptomyces clavuligerus NRRL 3585
Q8RPZ0
NRRL 3585
-
Purification (Commentary)
EC Number
Commentary
Organism
4.1.3.1
90% homogeneity
Streptomyces clavuligerus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.3.1
2.23
-
-
Streptomyces clavuligerus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
650335
Streptomyces clavuligerus
succinate + glyoxylate
-
650335
Streptomyces clavuligerus
?
4.1.3.1
isocitrate
-
650335
Streptomyces clavuligerus NRRL 3585
succinate + glyoxylate
-
650335
Streptomyces clavuligerus NRRL 3585
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.1
expressed in Escherichia coli
Streptomyces clavuligerus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
475000
-
calculated from amino acid sequence
Streptomyces clavuligerus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.1.3.1
isocitrate
Streptomyces clavuligerus
-
succinate + glyoxylate
-
Streptomyces clavuligerus
?
4.1.3.1
isocitrate
Streptomyces clavuligerus NRRL 3585
-
succinate + glyoxylate
-
Streptomyces clavuligerus NRRL 3585
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.1
90% homogeneity
Streptomyces clavuligerus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.3.1
2.23
-
-
Streptomyces clavuligerus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
650335
Streptomyces clavuligerus
succinate + glyoxylate
-
650335
Streptomyces clavuligerus
?
4.1.3.1
isocitrate
-
650335
Streptomyces clavuligerus NRRL 3585
succinate + glyoxylate
-
650335
Streptomyces clavuligerus NRRL 3585
?