BRENDA - Enzyme Database show

The macrophage-induced gene (mig) of Mycobacterium avium encodes a medium-chain acyl-coenzyme A synthetase

Morsczeck, C.; Berger, S.; Plum, G.; Biochim. Biophys. Acta 1521, 59-65 (2001)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
6.2.1.2
a plasmid with a His-tagged fusion protein is constructed for expression in Escherichia coli
Mycobacterium avium
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
6.2.1.2
2-hydroxydodecanoic acid
-
Mycobacterium avium
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.2.1.2
0.02
-
dodecanoate
-
Mycobacterium avium
6.2.1.2
0.036
-
ATP
-
Mycobacterium avium
6.2.1.2
0.045
-
CoA
-
Mycobacterium avium
6.2.1.2
0.285
-
Octanoate
-
Mycobacterium avium
6.2.1.2
1.775
-
Decanoate
-
Mycobacterium avium
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.2.1.2
additional information
Mycobacterium avium
the enzyme is involved in the metabolism of fatty acid during mycobacterial survival in macrophages
?
-
Mycobacterium avium
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
6.2.1.2
Mycobacterium avium
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
6.2.1.2
-
Mycobacterium avium
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.2.1.2
ATP + 2-aminobenzoate + CoA
1.4% of the activity with dodecanoate
650334
Mycobacterium avium
AMP + diphosphate + 2-aminobenzoyl-CoA
-
650334
Mycobacterium avium
?
6.2.1.2
ATP + arachidonic acid + CoA
5.2% of the activity with dodecanoate
650334
Mycobacterium avium
AMP + diphosphate + arachidonoyl-CoA
-
650334
Mycobacterium avium
?
6.2.1.2
ATP + benzoate + CoA
2.3% of the activity with dodecanoate
650334
Mycobacterium avium
AMP + diphosphate + benzoyl-CoA
-
650334
Mycobacterium avium
?
6.2.1.2
ATP + decanoate + CoA
substrate with the highest activity
650334
Mycobacterium avium
AMP + diphosphate + decanoyl-CoA
-
650334
Mycobacterium avium
?
6.2.1.2
ATP + dodecanoate + CoA
31% of the activity with dodecanoate
650334
Mycobacterium avium
AMP + diphosphate + dodecanoyl-CoA
-
650334
Mycobacterium avium
?
6.2.1.2
ATP + heptanoate + CoA
34% of the activity with dodecanoate
650334
Mycobacterium avium
AMP + diphosphate + heptanoyl-CoA
-
650334
Mycobacterium avium
?
6.2.1.2
ATP + hexanoate + CoA
35% of the activity with dodecanoate
650334
Mycobacterium avium
AMP + diphosphate + hexanoyl-CoA
-
650334
Mycobacterium avium
?
6.2.1.2
ATP + linolenic acid + CoA
8.3% of the activity with dodecanoate
650334
Mycobacterium avium
AMP + diphosphate + linolenoyl-CoA
-
650334
Mycobacterium avium
?
6.2.1.2
ATP + m-hydroxybenzoate + CoA
0.8% of the activity with dodecanoate
650334
Mycobacterium avium
AMP + diphosphate + m-hydroxybenzoyl-CoA
-
650334
Mycobacterium avium
?
6.2.1.2
ATP + octanoate + CoA
31% of the activity with dodecanoate
650334
Mycobacterium avium
AMP + diphosphate + octanoyl-CoA
-
650334
Mycobacterium avium
?
6.2.1.2
ATP + pentanoate + CoA
1.6% of the activity with dodecanoate
650334
Mycobacterium avium
AMP + diphosphate + pentanoyl-CoA
-
650334
Mycobacterium avium
?
6.2.1.2
ATP + tetradecanoate + CoA
3.9% of the activity with dodecanoate
650334
Mycobacterium avium
AMP + diphosphate + tetradecanoyl-CoA
-
650334
Mycobacterium avium
?
6.2.1.2
ATP + tridecanoate + CoA
15% of the activity with dodecanoate
650334
Mycobacterium avium
AMP + diphosphate + tridecanoyl-CoA
-
650334
Mycobacterium avium
?
6.2.1.2
additional information
the enzyme is involved in the metabolism of fatty acid during mycobacterial survival in macrophages
650334
Mycobacterium avium
?
-
650334
Mycobacterium avium
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
6.2.1.2
a plasmid with a His-tagged fusion protein is constructed for expression in Escherichia coli
Mycobacterium avium
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
6.2.1.2
2-hydroxydodecanoic acid
-
Mycobacterium avium
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
6.2.1.2
0.02
-
dodecanoate
-
Mycobacterium avium
6.2.1.2
0.036
-
ATP
-
Mycobacterium avium
6.2.1.2
0.045
-
CoA
-
Mycobacterium avium
6.2.1.2
0.285
-
Octanoate
-
Mycobacterium avium
6.2.1.2
1.775
-
Decanoate
-
Mycobacterium avium
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
6.2.1.2
additional information
Mycobacterium avium
the enzyme is involved in the metabolism of fatty acid during mycobacterial survival in macrophages
?
-
Mycobacterium avium
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
6.2.1.2
-
Mycobacterium avium
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
6.2.1.2
ATP + 2-aminobenzoate + CoA
1.4% of the activity with dodecanoate
650334
Mycobacterium avium
AMP + diphosphate + 2-aminobenzoyl-CoA
-
650334
Mycobacterium avium
?
6.2.1.2
ATP + arachidonic acid + CoA
5.2% of the activity with dodecanoate
650334
Mycobacterium avium
AMP + diphosphate + arachidonoyl-CoA
-
650334
Mycobacterium avium
?
6.2.1.2
ATP + benzoate + CoA
2.3% of the activity with dodecanoate
650334
Mycobacterium avium
AMP + diphosphate + benzoyl-CoA
-
650334
Mycobacterium avium
?
6.2.1.2
ATP + decanoate + CoA
substrate with the highest activity
650334
Mycobacterium avium
AMP + diphosphate + decanoyl-CoA
-
650334
Mycobacterium avium
?
6.2.1.2
ATP + dodecanoate + CoA
31% of the activity with dodecanoate
650334
Mycobacterium avium
AMP + diphosphate + dodecanoyl-CoA
-
650334
Mycobacterium avium
?
6.2.1.2
ATP + heptanoate + CoA
34% of the activity with dodecanoate
650334
Mycobacterium avium
AMP + diphosphate + heptanoyl-CoA
-
650334
Mycobacterium avium
?
6.2.1.2
ATP + hexanoate + CoA
35% of the activity with dodecanoate
650334
Mycobacterium avium
AMP + diphosphate + hexanoyl-CoA
-
650334
Mycobacterium avium
?
6.2.1.2
ATP + linolenic acid + CoA
8.3% of the activity with dodecanoate
650334
Mycobacterium avium
AMP + diphosphate + linolenoyl-CoA
-
650334
Mycobacterium avium
?
6.2.1.2
ATP + m-hydroxybenzoate + CoA
0.8% of the activity with dodecanoate
650334
Mycobacterium avium
AMP + diphosphate + m-hydroxybenzoyl-CoA
-
650334
Mycobacterium avium
?
6.2.1.2
ATP + octanoate + CoA
31% of the activity with dodecanoate
650334
Mycobacterium avium
AMP + diphosphate + octanoyl-CoA
-
650334
Mycobacterium avium
?
6.2.1.2
ATP + pentanoate + CoA
1.6% of the activity with dodecanoate
650334
Mycobacterium avium
AMP + diphosphate + pentanoyl-CoA
-
650334
Mycobacterium avium
?
6.2.1.2
ATP + tetradecanoate + CoA
3.9% of the activity with dodecanoate
650334
Mycobacterium avium
AMP + diphosphate + tetradecanoyl-CoA
-
650334
Mycobacterium avium
?
6.2.1.2
ATP + tridecanoate + CoA
15% of the activity with dodecanoate
650334
Mycobacterium avium
AMP + diphosphate + tridecanoyl-CoA
-
650334
Mycobacterium avium
?
6.2.1.2
additional information
the enzyme is involved in the metabolism of fatty acid during mycobacterial survival in macrophages
650334
Mycobacterium avium
?
-
650334
Mycobacterium avium
?