BRENDA - Enzyme Database

Enzymological properties of the LPP1-encoded lipid phosphatase from Saccharomyces cerevisiae

Furneisen, J.M.; Carman, G.M.; Biochim. Biophys. Acta 1484, 71-82 (2000)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.1.3.4
overexpression in Sf-9 insect cells
Saccharomyces cerevisiae
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
Ca2+
IC50: 15 mM, reaction with phosphatidic acid. IC50: 7.1 mM, reaction with diacylglycerol diphosphate. IC50: 8.2 mM, reaction with lysophosphatidic acid
Saccharomyces cerevisiae
3.1.3.4
Co2+
IC50: 0.029 mM, reaction with phosphatidic acid. IC50: 1.1 mM, reaction with diacylglycerol diphosphate. IC50: 1.2 mM, reaction with lysophosphatidic acid
Saccharomyces cerevisiae
3.1.3.4
diacylglycerol diphosphate
competitive with respect to phosphatidic acid
Saccharomyces cerevisiae
3.1.3.4
HgCl2
-
Saccharomyces cerevisiae
3.1.3.4
Mg2+
inhibition of reaction with diacylglycerol diphosphate. Little effect on reaction with lysophosphatidic acid and phosphatidic acid
Saccharomyces cerevisiae
3.1.3.4
Mn2+
IC50: 0.066 mM, reaction with phosphatidic acid. IC50: 0.01 mM, reaction with diacylglycerol diphosphate. IC50: 0.091 mM, reaction with lysophosphatidic acid
Saccharomyces cerevisiae
3.1.3.4
NaF
5 mM, 21% inhibition of reaction with phosphatidic acid, 30% inhibition of reaction with diacylglycerol diphosphate and 44% inhibition of the reaction with lysophosphatidic acid
Saccharomyces cerevisiae
3.1.3.4
NEM
IC50: 0.23 mM, reaction with phosphatidic acid. IC50: 0.12 mM, reaction with diacylglycerol diphosphate. IC50: 0.17 mM, reaction with lysophosphatidic acid
Saccharomyces cerevisiae
3.1.3.4
p-chloromercuriphenylsulfonic acid
-
Saccharomyces cerevisiae
3.1.3.4
Phenylglyoxal
IC50: 7 mM, reaction with phosphatidic acid. IC50: 3.4 mM, reaction with diacylglycerol diphosphate. IC50: 2.5 mM, reaction with lysophosphatidic acid
Saccharomyces cerevisiae
3.1.3.4
phosphatidic acid
competitive with respect to diacylglycerol diphosphate
Saccharomyces cerevisiae
3.1.3.4
propranolol
IC50: 2.51 mM, reaction with phosphatidic acid. IC50: 1.68 mM, reaction with diacylglycerol diphosphate. IC50: 7.17 mM, reaction with lysophosphatidic acid
Saccharomyces cerevisiae
3.1.3.4
ZnCl2
-
Saccharomyces cerevisiae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.4
additional information
-
additional information
Km-value for phosphatidic acid: 0.05 mol%, Km-value for diacylglycerol diphosphate: 0.07 mol%, KM-value for lysophosphatidate : 0.08 mol%
Saccharomyces cerevisiae
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
membrane
associated with
Saccharomyces cerevisiae
16020
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.4
additional information
Mg2+-independent enzyme
Saccharomyces cerevisiae
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.4
Saccharomyces cerevisiae
-
two genes DPP1 and LPP1 encode Mg2+-independent phosphatidic acid phosphatase activities
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
diacylglycerol diphosphate + H2O
-
650322
Saccharomyces cerevisiae
?
-
650322
Saccharomyces cerevisiae
?
3.1.3.4
lysophosphatidate + H2O
-
650322
Saccharomyces cerevisiae
monoacylglycerol + phosphate
-
650322
Saccharomyces cerevisiae
?
3.1.3.4
phosphatidate + H2O
-
650322
Saccharomyces cerevisiae
1,2-diacyl-sn-glycerol + phosphate
-
650322
Saccharomyces cerevisiae
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.4
7
-
reaction with diacylglycerol diphosphate or lysophosphatidate
Saccharomyces cerevisiae
3.1.3.4
7.5
-
reaction with phosphatidic acid
Saccharomyces cerevisiae
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.3.4
5
8
pH 5.0: about 40% of maximal activity, pH 8.0: about 40% of maximal activity, reaction with lysophosphatidic acid
Saccharomyces cerevisiae
3.1.3.4
5.5
8
pH 5.0: about 45% of maximal activity, pH 8.0: about 35% of maximal activity, reaction with diacylglycerol diphosphate
Saccharomyces cerevisiae
3.1.3.4
5.5
9
pH 5.0: about 55% of maximal activity, pH 9.0: about 80% of maximal activity, reaction with phosphatidic acid
Saccharomyces cerevisiae
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.1.3.4
additional information
-
additional information
Ki-value for phosphatidic acid competitive with respect to diacylglycerol diphosphate : 0.12 mol%. Ki-value for diacylglycerol diphosphate competitive with respect to phosphatidic acid: 0.12 mol%
Saccharomyces cerevisiae
IC50 Value
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
3.1.3.4
0.091
-
IC50: 0.066 mM, reaction with phosphatidic acid. IC50: 0.01 mM, reaction with diacylglycerol diphosphate. IC50: 0.091 mM, reaction with lysophosphatidic acid
Saccharomyces cerevisiae
Mn2+
3.1.3.4
0.17
-
IC50: 0.23 mM, reaction with phosphatidic acid. IC50: 0.12 mM, reaction with diacylglycerol diphosphate. IC50: 0.17 mM, reaction with lysophosphatidic acid
Saccharomyces cerevisiae
NEM
3.1.3.4
1.2
-
IC50: 0.029 mM, reaction with phosphatidic acid. IC50: 1.1 mM, reaction with diacylglycerol diphosphate. IC50: 1.2 mM, reaction with lysophosphatidic acid
Saccharomyces cerevisiae
Co2+
3.1.3.4
2.5
-
IC50: 7 mM, reaction with phosphatidic acid. IC50: 3.4 mM, reaction with diacylglycerol diphosphate. IC50: 2.5 mM, reaction with lysophosphatidic acid
Saccharomyces cerevisiae
Phenylglyoxal
3.1.3.4
7.17
-
IC50: 2.51 mM, reaction with phosphatidic acid. IC50: 1.68 mM, reaction with diacylglycerol diphosphate. IC50: 7.17 mM, reaction with lysophosphatidic acid
Saccharomyces cerevisiae
propranolol
3.1.3.4
8.2
-
IC50: 15 mM, reaction with phosphatidic acid. IC50: 7.1 mM, reaction with diacylglycerol diphosphate. IC50: 8.2 mM, reaction with lysophosphatidic acid
Saccharomyces cerevisiae
Ca2+
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.3.4
overexpression in Sf-9 insect cells
Saccharomyces cerevisiae
IC50 Value (protein specific)
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
3.1.3.4
0.091
-
IC50: 0.066 mM, reaction with phosphatidic acid. IC50: 0.01 mM, reaction with diacylglycerol diphosphate. IC50: 0.091 mM, reaction with lysophosphatidic acid
Saccharomyces cerevisiae
Mn2+
3.1.3.4
0.17
-
IC50: 0.23 mM, reaction with phosphatidic acid. IC50: 0.12 mM, reaction with diacylglycerol diphosphate. IC50: 0.17 mM, reaction with lysophosphatidic acid
Saccharomyces cerevisiae
NEM
3.1.3.4
1.2
-
IC50: 0.029 mM, reaction with phosphatidic acid. IC50: 1.1 mM, reaction with diacylglycerol diphosphate. IC50: 1.2 mM, reaction with lysophosphatidic acid
Saccharomyces cerevisiae
Co2+
3.1.3.4
2.5
-
IC50: 7 mM, reaction with phosphatidic acid. IC50: 3.4 mM, reaction with diacylglycerol diphosphate. IC50: 2.5 mM, reaction with lysophosphatidic acid
Saccharomyces cerevisiae
Phenylglyoxal
3.1.3.4
7.17
-
IC50: 2.51 mM, reaction with phosphatidic acid. IC50: 1.68 mM, reaction with diacylglycerol diphosphate. IC50: 7.17 mM, reaction with lysophosphatidic acid
Saccharomyces cerevisiae
propranolol
3.1.3.4
8.2
-
IC50: 15 mM, reaction with phosphatidic acid. IC50: 7.1 mM, reaction with diacylglycerol diphosphate. IC50: 8.2 mM, reaction with lysophosphatidic acid
Saccharomyces cerevisiae
Ca2+
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.3.4
Ca2+
IC50: 15 mM, reaction with phosphatidic acid. IC50: 7.1 mM, reaction with diacylglycerol diphosphate. IC50: 8.2 mM, reaction with lysophosphatidic acid
Saccharomyces cerevisiae
3.1.3.4
Co2+
IC50: 0.029 mM, reaction with phosphatidic acid. IC50: 1.1 mM, reaction with diacylglycerol diphosphate. IC50: 1.2 mM, reaction with lysophosphatidic acid
Saccharomyces cerevisiae
3.1.3.4
diacylglycerol diphosphate
competitive with respect to phosphatidic acid
Saccharomyces cerevisiae
3.1.3.4
HgCl2
-
Saccharomyces cerevisiae
3.1.3.4
Mg2+
inhibition of reaction with diacylglycerol diphosphate. Little effect on reaction with lysophosphatidic acid and phosphatidic acid
Saccharomyces cerevisiae
3.1.3.4
Mn2+
IC50: 0.066 mM, reaction with phosphatidic acid. IC50: 0.01 mM, reaction with diacylglycerol diphosphate. IC50: 0.091 mM, reaction with lysophosphatidic acid
Saccharomyces cerevisiae
3.1.3.4
NaF
5 mM, 21% inhibition of reaction with phosphatidic acid, 30% inhibition of reaction with diacylglycerol diphosphate and 44% inhibition of the reaction with lysophosphatidic acid
Saccharomyces cerevisiae
3.1.3.4
NEM
IC50: 0.23 mM, reaction with phosphatidic acid. IC50: 0.12 mM, reaction with diacylglycerol diphosphate. IC50: 0.17 mM, reaction with lysophosphatidic acid
Saccharomyces cerevisiae
3.1.3.4
p-chloromercuriphenylsulfonic acid
-
Saccharomyces cerevisiae
3.1.3.4
Phenylglyoxal
IC50: 7 mM, reaction with phosphatidic acid. IC50: 3.4 mM, reaction with diacylglycerol diphosphate. IC50: 2.5 mM, reaction with lysophosphatidic acid
Saccharomyces cerevisiae
3.1.3.4
phosphatidic acid
competitive with respect to diacylglycerol diphosphate
Saccharomyces cerevisiae
3.1.3.4
propranolol
IC50: 2.51 mM, reaction with phosphatidic acid. IC50: 1.68 mM, reaction with diacylglycerol diphosphate. IC50: 7.17 mM, reaction with lysophosphatidic acid
Saccharomyces cerevisiae
3.1.3.4
ZnCl2
-
Saccharomyces cerevisiae
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.1.3.4
additional information
-
additional information
Ki-value for phosphatidic acid competitive with respect to diacylglycerol diphosphate : 0.12 mol%. Ki-value for diacylglycerol diphosphate competitive with respect to phosphatidic acid: 0.12 mol%
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.4
additional information
-
additional information
Km-value for phosphatidic acid: 0.05 mol%, Km-value for diacylglycerol diphosphate: 0.07 mol%, KM-value for lysophosphatidate : 0.08 mol%
Saccharomyces cerevisiae
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
membrane
associated with
Saccharomyces cerevisiae
16020
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.4
additional information
Mg2+-independent enzyme
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
diacylglycerol diphosphate + H2O
-
650322
Saccharomyces cerevisiae
?
-
650322
Saccharomyces cerevisiae
?
3.1.3.4
lysophosphatidate + H2O
-
650322
Saccharomyces cerevisiae
monoacylglycerol + phosphate
-
650322
Saccharomyces cerevisiae
?
3.1.3.4
phosphatidate + H2O
-
650322
Saccharomyces cerevisiae
1,2-diacyl-sn-glycerol + phosphate
-
650322
Saccharomyces cerevisiae
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.4
7
-
reaction with diacylglycerol diphosphate or lysophosphatidate
Saccharomyces cerevisiae
3.1.3.4
7.5
-
reaction with phosphatidic acid
Saccharomyces cerevisiae
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.3.4
5
8
pH 5.0: about 40% of maximal activity, pH 8.0: about 40% of maximal activity, reaction with lysophosphatidic acid
Saccharomyces cerevisiae
3.1.3.4
5.5
8
pH 5.0: about 45% of maximal activity, pH 8.0: about 35% of maximal activity, reaction with diacylglycerol diphosphate
Saccharomyces cerevisiae
3.1.3.4
5.5
9
pH 5.0: about 55% of maximal activity, pH 9.0: about 80% of maximal activity, reaction with phosphatidic acid
Saccharomyces cerevisiae