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Literature summary extracted from

  • Siigur, E.; Samel, M.; Tonismaegi, K.; Subbi, J.; Reintamm, T.; Siigur, J.
    Isolation, properties and N-terminal amino acid sequence of a factor V activator from Vipera lebetina (levantine viper) snake venom (1998), Biochim. Biophys. Acta, 1429, 239-248.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.21.95 diisopropylfluorophosphate
-
 Macrovipera lebetina

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.95 28400
-
 gel filtration, MALDI-TOF mass spectrometry Macrovipera lebetina
3.4.21.95 30000
-
 1 * 30000, SDS-PAGE Macrovipera lebetina

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.95 factor V + H2O Macrovipera lebetina aside from factor V, no other protein substrate yet been identified ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.95 Macrovipera lebetina
-
 Levantine viper
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.21.95 glycoprotein
-
 Macrovipera lebetina

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.95
-
 Macrovipera lebetina

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.95 1.2
-
 benzoylarginine ethyl ester as substrate Macrovipera lebetina
3.4.21.95 4.9
-
 Pro-Phe-Arg-4-methylcoumarin 7-amide as substrate Macrovipera lebetina

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.95 Benzoylarginine ethyl ester + H2O
-
 Macrovipera lebetina ?
-
 ?
3.4.21.95 factor V + H2O
-
 Macrovipera lebetina ?
-
 ?
3.4.21.95 factor V + H2O aside from factor V, no other protein substrate yet been identified Macrovipera lebetina ?
-
 ?
3.4.21.95 additional information hydrolyzes several synthetic arginine ester substrates Macrovipera lebetina ?
-
 ?
3.4.21.95 Pro-Phe-Arg-4-methylcoumarin 7-amide + H2O
-
 Macrovipera lebetina ?
-
 ?
3.4.21.95 tosylarginine methyl ester + H2O
-
 Macrovipera lebetina ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.95 monomer 1 * 30000, SDS-PAGE Macrovipera lebetina

Synonyms

EC Number Synonyms Comment Organism
3.4.21.95 VLFVA
-
 Macrovipera lebetina

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.95 70
-
 thermostable, heating for 20 min does not alter the arginine esterase activity of the enzyme Macrovipera lebetina

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.21.95 Macrovipera lebetina analytical isoelectric focusing studies reveals a protein band near the cathode, pI higer than 9.3
-
 9.3